Differentiation of Candida dubliniensis from Candida albicans with the use of killer toxins

Scheid, Liliane Alves; Mario, Débora Alves Nunes; Heins‐Vaccari, Elisabeth Maria; Santúrio, Jânio Morais; Alves, Sydney Hartz

doi:10.1590/s0036-46652010000300009

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…It has been possible to differentiate between species of pathogenic yeasts using the killer system (Coutinho and Paula 1998;Scheid et al 2010;Boekhout and Scorzetti 1997), and we suggest that it could be done for Candida species as well if a more taxonomically diverse panel of killer strains is employed. This would represent a rapid and low cost diagnostic tool.…”

Section: Discussionmentioning

confidence: 99%

Identification and susceptibility of clinical isolates of Candida spp. to killer toxins

et al. 2018

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Although invasive infections and mortality caused by Candida species are increasing among compromised patients, resistance to common antifungal agents is also an increasing problem. We analyzed 60 yeasts isolated from patients with invasive candidiasis using a PCR/RFLP strategy based on the internal transcribed spacer (ITS2) region to identify different Candida pathogenic species. PCR analysis was performed from genomic DNA with a primer pair of the ITS2-5.8S rDNA region. PCR-positive samples were characterized by RFLP. Restriction resulted in 23 isolates identified as C. albicans using AlwI, 24 isolates as C. parapsilosis using RsaI, and 13 as C. tropicalis using XmaI. Then, a group of all isolates were evaluated for their susceptibility to a panel of previously described killer yeasts, resulting in 75% being susceptible to at least one killer yeast while the remaining were not inhibited by any strain. C. albicans was the most susceptible group while C. tropicalis had the fewest inhibitions. No species-specific pattern of inhibition was obtained with this panel of killer yeasts. Metschnikowia pulcherrima, Pichia kluyveri and Wickerhamomyces anomalus were the strains that inhibited the most isolates of Candida spp. Keywords

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Section: Discussionmentioning

confidence: 99%

Identification and susceptibility of clinical isolates of Candida spp. to killer toxins

et al. 2018

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Current awareness on yeast

2010

Yeast

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Yeast Yeast 20I0; 27: I07I-I079 Published online in Wiley Online Library (wileyonlinelibrary.com). DOI: I0.I002/yea.I724 Current awareness on yeast(5 weeks journals -Search completed at 13th. Oct. 2010) In order to keep subscribers up-to-date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly-published material on yeasts. Each bibliography is divided into I0 sections. I Reviews; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics & Genomics; 8 Physiology; 9 Medical Mycology; I0 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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Biology of Killer Yeast and Technological Implications

Bajaj

Singh

2017

Yeast Diversity in Human Welfare

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Differentiation of Candida dubliniensis from Candida albicans with the use of killer toxins

Cited by 4 publications

References 13 publications

Identification and susceptibility of clinical isolates of Candida spp. to killer toxins

Identification and susceptibility of clinical isolates of Candida spp. to killer toxins

Current awareness on yeast

Biology of Killer Yeast and Technological Implications

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