Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Tiba, Monique Ribeiro; Yano, Tomomasa; Leite, Domingos da Silva

doi:10.1590/s0036-46652008000500001

Cited by 70 publications

(62 citation statements)

References 26 publications

(46 reference statements)

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“…The aerobactin prevalence in E. coli strains is also highly variable in the literature, from 26% to 85% (5,11,13,15,28,39,40). In this study, the prevalence of aerobactin was up to 28% but not significantly different between the PN and Cys isolates, coinciding with some reports (11,15) but not with others (5,13).…”

Section: Discussionsupporting

confidence: 74%

The Role of Host Factors and Bacterial Virulence Genes in the Development of Pyelonephritis Caused by Escherichia coli in Renal Transplant Recipients

Siliano

Rocha

Medina-Pestana

et al. 2010

Clinical Journal of the American Society of Nephrology

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Background and objectives: The aim of this study was to determine the role of host factors and bacterial virulence genes in the development of pyelonephritis caused by Escherichia coli in renal transplant (Tx) recipients.Design, setting, participants, & measurements: A total of 328 E. coli isolates from cases of cystitis (Cys; n ‫؍‬ 239) or pyelonephritis (PN; n ‫؍‬ 89), with 169 from renal Tx recipients, were subjected to molecular analyses to identify P-fimbria subunits (PapC, PapG II, and PapGIII), G-and M-fimbriae, and aerobactin. The presence of antibiotic resistance was also determined. Parameters such as gender, age, immunosuppression regimens, causes of ESRD, kidney donor, intraoperative anastomosis, use of double J stent, trimethoprim/sulfamethoxazole (TMP/SMZ) prophylaxis, and time after Tx were evaluated.Results: A multivariate analysis showed a significant association between PN and renal Tx. In renal Tx recipients, the risk of occurrence of PN was significantly higher among males and for those no longer receiving TMP/SMZ prophylaxis. E. coli strains isolated from PN presented a lower prevalence of papGIII and lower rates of resistance to pipemidic acid. Although papGII was more prevalent in PN than in Cys, it was not independently associated with PN.Conclusions: These findings suggested that renal Tx increases the risk for PN, and the male sex represented a host factor independently associated with risk, whereas the prophylaxis with TMP/SMZ was protective. The lack of papGIII and low resistance to first-generation quinolones were bacterial-independent risk factors for PN in Tx.

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Section: Discussionsupporting

confidence: 74%

The Role of Host Factors and Bacterial Virulence Genes in the Development of Pyelonephritis Caused by Escherichia coli in Renal Transplant Recipients

Siliano

Rocha

Medina-Pestana

et al. 2010

Clinical Journal of the American Society of Nephrology

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“…Three cases of complicated infections such as pyelonephritis, prostatitis, and sepsis were associated with PAI ICFT073. In other studies, these virulence genes and markers were also identified among patients with complicated UTIs and isolates from women with pyelonephritis [21,22], which could explain why this marker is found more frequently in extremely virulent strains.…”

Section: Discussionmentioning

confidence: 81%

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

Firoozeh

Soleimani-Moorchekhorti

Zibaei

2017

J Infect Dev Ctries

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Introduction: Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs) .The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters. Methodology: A descriptive cross-sectional study was designed and from December 2014 to April 2015, 78 non-duplicate E. coli were collected from hospitalized patients with UTIs, including patients with and without indwelling urinary catheters. Two multiplex polymerase chain reaction (PCR) assays were performed to evaluate the presence of the eight most studied PAIs (I 536 were not detected in the UPEC strains. Conclusions: The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. PAI ICFT073, which was found in association with pyelonephritis, prostatitis, and sepsis, could be considered as a target for medical interventions.

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“…Tiba et al conducted a study on the genetics of virulence genes of pathogenic E. coli from patients with cystitis. The frequency of virulence genes fimH, papC, sfa, and afa was 97.5%, 32.7%, 27.8%, and 6.2%, respectively [34]. In another study by Usein et al, the prevalence rates of fimH, sfa/foc, papC, and afa in Romanian adults with UTI was 86%, 23%, 36%, and 14%, respectively [35].…”

Section: Discussionmentioning

confidence: 96%

Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia

Yandag

Gunregjav

Altantsetseg

et al. 2017

J Infect Dev Ctries

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Introduction: The severity of urinary tract infection (UTI) produced by uropathogenic Escherichia coli (UPEC) is due to the expression of a wide spectrum of virulence genes. E. coli strains were divided into four phylogenetic groups (A, B1, B2 and D) based on their virulence genes. The present study aimed to assess the relationship between virulence genes, phylogenetic groups, and antibiotic resistance of UPEC. Methodology: A total of 148 E. coli were tested for antimicrobial resistance against 10 drugs using the disk diffusion method. The isolates were screened by polymerase chain reaction (PCR) for detection of virulence genes and categorized into the four major phylogenetic groups. Results: Phylogenetic group B2 was predominant (33.8%), followed by D (28.4%), A (19.6), and B1 (18.2%). A higher prevalence of fimH (89.9%), fyuA (70.3%), traT (66.2%), iutA (62.2%), kpsMTII (58.8%), and aer (56.1%) genes were found in UPEC, indicating a putative role of adhesins, iron acquisition systems, and protectins that are main cause of UTIs. The most common antibiotic resistance was to cephalotin (85.1%), ampicillin (78.4%) and the least to nitrofurantoin (5.4%) and imipenem (2%). In total, 93.9% of isolates were multidrug resistant (MDR). Conclusions: This study showed that group B2 and D were the predominant phylogenetic groups and virulence-associated genes were mostly distributed in these groups. The virulence genes encoding components of adhesins, iron acquisition systems, and protectins were highly prevalent among antibiotic-resistant UPEC. Although the majority of strains are MDR, nitrofurantoin is the drug of choice for treatment of UTI patients in Ulaanbaatar.

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Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Cited by 70 publications

References 26 publications

The Role of Host Factors and Bacterial Virulence Genes in the Development of Pyelonephritis Caused by Escherichia coli in Renal Transplant Recipients

The Role of Host Factors and Bacterial Virulence Genes in the Development of Pyelonephritis Caused by Escherichia coli in Renal Transplant Recipients

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia

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