In vitro and in situ activation of the complement system by the fungus Lacazia loboi

Vilani-Moreno, Fátima Regina; Mozer, Érika; Sene, Ana Márcia Guedes de; Ferasçoli, Margarete de Oliveira; Pereira, Tânia Cristina; Miras, Márcia Garcia; Souza, Gláucia Heloísa de Paula; Belone, Andréa de Faria Fernandes

doi:10.1590/s0036-46652007000200006

Cited by 10 publications

(9 citation statements)

References 14 publications

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“…The cellular immune response using skin tests with fungal and bacterial antigens, suggest that patients with disease have partial deficiency of the cell immune response. The quantification of cytokines in the culture supernatant of mononuclear cells suggests the predominance of a Th2 profile corroborating the results obtained for tissue lesions and serum in which in situ deposits of immunoglobulins and complement and elevated serum levels of immunoglobulins were detected.…”

Section: Discussionsupporting

confidence: 86%

Nutritional status and immune response in murine experimental Jorge Lobo's disease

Barbosa

Diório

Pedrini

et al. 2015

Mycoses

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There are no studies investigating the role of nutritional status and immunity associated with Jorge Lobo's disease. The objective of this study was to evaluate the effects of protein-calorie malnutrition on the immune response of BALB/c mice inoculated with Lacazia loboi. In this study,the animals were divided into four groups: G1: inoculated with restricted diet, G2: not inoculated with restricted diet, G3: inoculated with regular diet, G4: not inoculated with regular diet. The animals of groups G1 and G2 were submitted to malnutrition for 20 days and once installed the animals were inoculated intradermally into the footpad. After 4 months, they were euthanised for the isolation of peritoneal lavage cells and removal of the footpad. The production of IL-2, IL-4, IL-10, IL-12, IFN-γ, TNF-α, H2 O2 and nitric oxide (NO) was evaluated in the peritoneal lavage cells. The footpad was evaluated regarding the size of macroscopic lesions, number of fungi and viability index. The results showed that the infection did not exert great influence on the body weight of the mice and previous malnutrition was an unfavourable factor for viability index, number of fungi, macroscopic lesion size in the footpad and production of H2 O2 , NO, IL-12, IL-10 and IFN-γ, suggesting that malnutrition significantly altered fungal activity and peritoneal cells. The results suggest considerable interaction between nutrition and immunity in Jorge Lobo's disease.

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Section: Discussionsupporting

confidence: 86%

Nutritional status and immune response in murine experimental Jorge Lobo's disease

Barbosa

Diório

Pedrini

et al. 2015

Mycoses

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“…28 , 35 Immunoglobulin and complemented characterization of the lesions are in agreement with the findings of a T helper 2 profile. 36 …”

Section: Introductionmentioning

confidence: 99%

“… 43 – 45 Another possibility is the participation of the complement system, as deposits of the C3c component on the fungal cell wall in histological sections obtained from patients with the mycosis could be demonstrated. 36 …”

Section: Introductionmentioning

confidence: 99%

Lobomycosis: epidemiology, clinical presentation, and management options

Francesconi¹,

Klein²,

Santos³

et al. 2014

TCRM

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Lobomycosis is a subcutaneous mycosis of chronic evolution caused by the Lacazia loboi fungus. Its distribution is almost exclusive in the Americas, and it has a particularly high prevalence in the Amazon basin. Cases of lobomycosis have been reported only in dolphins and humans. Its prevalence is higher among men who are active in the forest, such as rubber tappers, bushmen, miners, and Indian men. It is recognized that the traumatic implantation of the fungus on the skin is the route by which humans acquire this infection. The lesions affect mainly exposed areas such as the auricles and upper and lower limbs and are typically presented as keloid-like lesions. Currently, surgical removal is the therapeutic procedure of choice in initial cases. Despite the existing data and studies to date, the active immune mechanisms in this infection and its involvement in the control or development of lacaziosis have not been fully clarified. In recent years, little progress has been made in the appraisal of the epidemiologic aspects of the disease. So far, we have neither a population-based study nor any evaluation directed to the forest workers.

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“…645 These lesions evolve with keloidal scarring, seen as brightly eosinophilic collagen bundles. 601,647,[649][650][651][652][653] The fungi are PAS and methenamine silver positive and mucicarmine negative. 643 The fungi are identified in tissue as thick-walled, rounded or lemon-shaped, yeast-like cells, occurring either singly or in chains of budding cells (2-10 cells) (Figs.…”

Section: Lobomycosismentioning

confidence: 99%