Virulence factors of uropathogenic Escherichia coli from a University Hospital in Ribeirão Preto, São Paulo, Brazil

Santo, Edilene; Macedo, Cristiana Santos de; Marin, José Moacir

doi:10.1590/s0036-46652006000400002

Cited by 42 publications

(48 citation statements)

References 20 publications

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“…This frequency of pap in a cystitis strain agrees with literature data 26 . JOHNSON et al, 2005 11 , reported that cystitis isolates differed from pyelonephritis and prostatitis isolates by their lower prevalence of papA/C/EF/G, papG allele II and the papGII/III alleles combination.…”

supporting

confidence: 92%

“…Adhesins can also contribute to virulence, promoting colonization, invasion and replication within uroepithelial cells 22,23 . Besides bacterial adherence, several virulence factors may contribute to the pathogenicity of UPEC, facilitating the ability to adhere specifically to uroepithelial cells and the expression of other bacterial products to host tissues such as toxins, iron acquisition systems and host defense avoidance mechanisms 26 .…”

Section: Introductionmentioning

confidence: 99%

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Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Tiba

Yano

Leite

2008

Rev. Inst. Med. trop. S. Paulo

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Abbreviations: aerobactin (iucD), afimbrial adhesin (afaB/C), cytolethal distending toxin (cdtB), cytotoxic necrotizing factor type 1 (cnf-1), G adhesin classes of P fimbriae (papG alleles), group II capsule (kpsMTII), α-hemolysin (hly), minor structural subunits of P fimbriae (papE/F), outer membrane protein of P fimbrae (papC), polymerase chain reaction (PCR), S fimbriae (sfaC/D), type 1 fimbriae (fimH), urinary tract infection (UTI), uropathogenic specific protein (usp), uropathogenic Escherichia coli (UPEC), virulence factors (VFs SUMMARYAdhesins (P-fimbriae, S-fimbriae, type 1 fimbriae and afimbrial adhesin), toxins (α-hemolysin and cytotoxic necrotizing factor type 1), iron acquisition systems (aerobactin) and host defense avoidance mechanisms (capsule or lipopolysaccharide) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. In this work, 162 Uropathogenic Escherichia coli (UPEC) strains from patients with cystitis were genotypically characterized by polymerase chain reaction (PCR) assay. We developed three multiplex PCR assays for virulence-related genes papC, papE /F, papG alleles, fimH, sfa/foc, afaE, hly, usp, cdtB, iucD, and kpsMTII, all

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supporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Tiba

Yano

Leite

2008

Rev. Inst. Med. trop. S. Paulo

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“…Of the genes examined, four cell adhesion genes, papEF, fimH, focG and sfaS, one cell protection capsule gene, KpsMTKI, and both toxin alpha-hemolysin hlyA and cnfl genes were significantly more prevalent in strains from outpatients than from inpatients. These results are in agreement with those of Santo et al (32) who reported that different adhesion-related genes as well as the hemolysin gene related to tissue damage were twice as frequent in outpatients as inpatients. Our findings provide evidence that strains isolated from outpatients are generally more "armed" especially regarding adhesion, cell protection, and toxin genes.…”

Section: Discussionsupporting

confidence: 93%

Virulence Traits in Escherichia Coli Strains Isolated from Outpatients with Urinary Tract Infections

Longhi

Cossu

Iebba

et al. 2008

Int J Immunopathol Pharmacol

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This study aims to characterize phenotypic and genotypic virulence traits in Escherichia coli strains, isolated from outpatients with urinary tract infections, comparing with those obtained from inpatients. Information on the pathogenic behavior of the uropathogenic strains was obtained by monitoring different biological properties, such as autoagglutination, hemagglutination, adhesiveness to and invasion of human bladder (HT1376) cells, biofilm formation, phylogenetic grouping, and virulencerelated genes. The results show similar behavior in the two groups concerning autoagglutination, hemagglutination, and biofilm formation. None of the strains examined was invasive. However, in strains from outpatients there was an increased adhesion to HT1376 cells compared with clinical strains, a significant higher presence of genes codifying for adhesins and cell protection factors, and a lower proportion of strains belonging to Bl group. These findings add further information on the pathogenic traits of community E. coli, since strains isolated from the outpatients' group were differently "armed" in comparison with those of clinical cases, and more suitable to infect healthy individuals.Urinary tract infections (UTIs) are among the most common extraintestinal diseases in the developed world (I). Escherichia coli is by far the most common microrganism involved and isolated from about 50% of all nosocomial UTIs and 90% of outpatients (2). Urinary and commensal E. coli differ with respect to phylogenetic background and virulence (3). Phylogenetic analyses have shown that virulent E. coli clones, belonging to B2 and D groups (4-6), are preponderant in UTIs and possess specialized virulence factors conferring pathogenetic potential (3-4, 7). Molecular studies have contributed to the discovery ofthe determinants ofuropathogenic virulence that act in additive or synergic fashion, enhancing the cumulative impact of pathogenicity in UTIs (8-12). In fact, most uropathogenic E. coli strains exhibit definite virulence attributes, including adhesins, iron uptake systems, and synthesis of cytotoxins, that are lacking in commensal E. coli (II,(13)(14)(15).Adhesion of E. coli cells to uroepithelium, which may be associated with fimbrial structures, is a major feature of uropathogenic strains, since it protects the bacteria from urinary lavage, increasing their ability to colonize (16)(17). Following the selective adhesion to the mucosal uroepithelium or medical devices, E. coli cells grow as a biofilm and persist for a long time in the genitourinary tract, thus interfering with the chemioantibiotic therapy for bacterial eradication (18). Moreover, hemagglutination and aggregation,

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“…1 Extra intestinal Pathogenic E. coli (ExPEC) cause extra intestinal infections including urinary tract infections (UTIs), diverse intra-abdominal infections, pneumonia, surgical-site infections, meningitis, osteomyelitis, soft-tissue infections and bacteremia. 2,3 Uropathogenic E. coli (UPEC) strains are responsible for about 90% of all community-acquired UTIs and up to 50% of all nosocomial UTI. 4 The development of UTIs depends on anatomical factors, the integrity of host defense mechanisms, and the virulence of the infecting organisms.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antimicrobial susceptibility patterns of uropathogenic E. coli among people in Zakho, Iraq

2016

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Virulence factors of uropathogenic Escherichia coli from a University Hospital in Ribeirão Preto, São Paulo, Brazil

Cited by 42 publications

References 20 publications

Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Virulence Traits in Escherichia Coli Strains Isolated from Outpatients with Urinary Tract Infections

Prevalence and antimicrobial susceptibility patterns of uropathogenic E. coli among people in Zakho, Iraq

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