Anti-Taenia solium metacestode IgG antibodies in serum samples from inhabitants of a central-western region of Brazil

Oliveira, Heliana Batista de; Rodrigues, Raphael Silva; Barcelos, Ivanildes Solange da Costa; Silva, Luciana P.; Costa-Cruz, Júlia Maria

doi:10.1590/s0036-46652006000100011

Cited by 9 publications

(10 citation statements)

References 16 publications

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“…(Taeniidae family) and to the presence of common antigenic components between these species (Montenegro et al 1994). Cross-reactivity in S. stercoralis infections can be related to the infection of these individuals with both species (T. solium and S. stercoralis), as the city of Uberlândia and the "Triângulo Mineiro" region is considered endemic in terms of human cysticercosis, according to Silveira-Lacerda et al (2002) and Oliveira et al (2006), and hyperendemic for S. stercoralis according to Machado and Costa-Cruz (1998).…”

Section: Discussionmentioning

confidence: 99%

Application of Taenia saginata metacestodes as an alternative antigen for the serological diagnosis of human neurocysticercosis

et al. 2007

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Serological tests are an important tool for the diagnosis of neurocysticercosis (NCC), the disease caused by Taenia solium metacestodes. The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in the immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), and Western blotting (WB) tests compared with the metacestodes antigen of T. solium in serum samples. The samples were obtained from 130 individuals: 20 from patients with definitive NCC, Group 1; 18 from individuals infected by Taenia sp., Group 2; 40 from individuals infected by various parasites, Group 3; and 40 from healthy individuals, Group 4. The sensitivity of IFAT, ELISA, and WB using antigen obtained from T. solium applied to the patients of Group 1 yielded results of 85, 95, and 95%, respectively, for the three tests. When the tests were conducted using T. saginata metacestodes, results were 75, 80, and 85%, respectively. The specificity of IFAT, ELISA, and WB using antigen obtained from T. solium yielded results of 94.9, 88.8, and 93.9%. When the tests were conducted using T. saginata metacestodes, results were 95.9, 88.8, and 93.6%, respectively. No statistical differences for sensitivity or specificity among the antigens were found. In conclusion, the results indicated that T. saginata metacestodes can be used as an alternative antigen for NCC diagnosis.

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Section: Discussionmentioning

confidence: 99%

Application of Taenia saginata metacestodes as an alternative antigen for the serological diagnosis of human neurocysticercosis

et al. 2007

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“…Although our study was conducted with well‐documented samples from NC patients or from patients with heterologous infections or apparently healthy individuals, previous studies using whole metacestode extracts have demonstrated that either ELISA or immunoblot are useful tools for epidemiological screening of NC (Silveira‐Lacerda et al. 2002; Oliveira et al. 2006; Prestes‐Carneiro et al.…”

Section: Discussionmentioning

confidence: 99%

Assessment of antigenic fractions of varying hydrophobicity from Taenia solium metacestodes for the diagnosis of human neurocysticercosis

Machado

Santiago

Mineo

et al. 2007

Tropical Med Int Health

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Summaryobjective To evaluate the detergent and aqueous phases of crude saline extract (crude) of Taenia solium metacestodes extracted with Triton X-114, for the detection of immunoglobulin G (IgG) antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot for the laboratory diagnosis of human neurocysticercosis.method Serum samples were collected from 138 individuals and tested; 40 were diagnosed with neurocysticercosis (NC; group 1), 58 presented other parasitic diseases (group 2) and 40 were apparently healthy (group 3).results The detergent phase showed only one dominant component (70-50 kDa), while the aqueous phase showed four (116, 110, 97 and 77 kDa) by silver staining. ELISA sensitivity and specificity were 92.5% and 84.5% for crude phase, 92.5% and 93.3% for detergent phase and 72.5% and 72.6% for aqueous phase.conclusions The immunoblot of the antigens confirmed the results obtained by ELISA. Detergent phase purified from T. solium metacestodes was an important source of specific peptides and was efficient in detecting T. solium metacestodes antibodies in serum samples. This phase can be used for detecting IgG antibodies to T. solium metacestodes in seroepidemiological investigations and in the diagnostic screening of NC patients.

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“…was thus demonstrated (12,15). These antigens are responsible for the cross-reactivity between both parasites, even though previous studies using whole-metacestode extracts have demonstrated that both the ELISA and immunoblotting may be considered useful tools for epidemiological screening of NC (30,34,38). Therefore, as cross-reactivity between T. solium and E. granulosus occurs in screening tests such as the ELISA, additional assays are required to validate the results, preferentially by using alternative assay designs with purified and more specific antigens.…”

Section: Discussionmentioning

confidence: 86%

“…Considering that there is minimal phylogenetic proximity between Taenia sp. and Strongyloides sp., these results may be related to the presence of infections with both species (T. solium and S. stercoralis) in these individuals, since human cysticercosis is considered endemic (30,38) and S. stercoralis hyperendemic (26) in the city of Uberlândia within the Triângulo Mineiro region.…”

Section: Discussionmentioning

confidence: 97%

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

Oliveira

Machado

Mineo

et al. 2010

Clin Vaccine Immunol

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Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of T. saginata metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented Taenia sp. and other parasites, and 40 were apparently healthy individuals. The jacalin-and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64-to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this component suitable for use as a specific antigen for diagnosis of NC. To our knowledge, this is the first report showing the relevance of using the unbound ConA fraction of T. saginata metacestodes to diagnose NC. In conclusion, the results obtained herein clearly demonstrate that antigenic fractions without affinity to ConA, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC when tested by immunoblot assay.Taenia solium is a parasite whose larvae (metacestodes) may be located in the central nervous system of humans, causing neurocysticercosis (NC). Clinical manifestations are nonspecific and vary in severity, ranging from headaches, dizziness, and occasional seizures to a very severe neurological condition with intracranial hypertension or dementia (39,40,42).The diagnosis of NC is given by the combined analysis of clinical data and neuroimaging (computerized tomography and magnetic resonance imaging) as well as immunological and epidemiological data (10). From the neuroimaging findings of NC, only the presence of cystic lesions demonstrating the scolex should be considered pathognomonic (4). In many countries where T. solium is endemic, neuroimaging methods could be inaccessible and/or too expensive for the population at risk, often rural. Under these conditions, serology may provide a viable tool for diagnosis of the infection (6,16,18,35).As NC immunodiagnosis continues to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for the preparation of T. solium homologous antigen (33), alternative antigens, including heterologous antigens from Taenia saginata, have also been used with satisfactory results to diagnose human NC (29,31,32). T. saginata cysticerci in the cysti...

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Anti-Taenia solium metacestode IgG antibodies in serum samples from inhabitants of a central-western region of Brazil

Cited by 9 publications

References 16 publications

Application of Taenia saginata metacestodes as an alternative antigen for the serological diagnosis of human neurocysticercosis

Application of Taenia saginata metacestodes as an alternative antigen for the serological diagnosis of human neurocysticercosis

Assessment of antigenic fractions of varying hydrophobicity from Taenia solium metacestodes for the diagnosis of human neurocysticercosis

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

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