Standardization and uses of IgG antibodies avidity assays in the laboratorial diagnosis of animal toxoplasmosis

Abstract

SUMMARY OF THESISMEIRELES, Luciana Regina -Padronização e aplicações da avidez de anticorpos IgG no diagnóstico laboratorial da toxoplasmose animal.

Occurrence of Toxoplasmosis in Baquba City, Diyala, Iraq

“…The results of the current study revealed that the highest figure in positive reactions were in age group (16)(17)(18)(19)(20) 55\205; 26.8% , followed by (31)(32)(33)(34)(35) , 42\ 205; 20.5%, and the lowest figures was in age groups (56-70) 2\205; 0.97%. This can attributed to the numbers of peoples examined.…”

“…Five hundred 500 serum samples were collected, represented 285 adult women suffered from hormonal and pregnancy disturbances; 03 young women, 49 young men, and 203 mature men healthy or suffered from general disease conditions, or accidental cases. The sera were submitted for LAT and ELISA tests (Table 1) LAT test in which, Antigen coated polystyrene latex particles were used, suitable for screening large numbers of samples [18][19][20]*. In ELISA, soluble antigen is coated to micro titer plates and serum is added to form an antigen-antibody complex (if specific antibodies are present) a secondary enzyme -linked antibody specific to the host species is added to detect antigen-antibody complex** [21].…”

“…Results revealed that the highest figure in positive reactions were in age group (16)(17)(18)(19)(20), 55\205; 26.8% , followed by (31-35) 42\ 205; 20.5%, and the lowest numbers were in age groups (56-70) 2\205; 0.97% (Table -5-).…”

“…and only 10 men showed positive reaction to IgM in ELISA. There were significant differences between age groups as (18)(19)(20)(21)(22)(23)(24)(25) and (26)(27)(28)(29)(30)(31)(32) year the highest percentage and (50)(51)(52)(53)(54)(55)(56)(57) was the lowest. However, [53] showed that male more susceptible than female to many seropositive parasites Al-Ghezy [54] referred that the highest infection rate has scored in age group (36-40) years 30.5%.…”

“…In the examining of 400 serum samples of aborted women by using ELISA test, 92 samples 23% and the highest infection rate has scored in age group (36-40) years 30.5%. In a study of prevalence of toxoplasmosis in pregnant women in Al-Muthana Province -Iraq, [55] found that there was significant effect of age on proportion rate which increase directly with age, highest infection rates were in (35)(36)(37)(38)(39) age group, while lowest at (15)(16)(17)(18)(19) age group. The increase in the level of toxoplasmosis infection with increasing age was also confirmed by other studies.…”