A simple and cheaper in house varicella zoster virus antibody indirect ELISA

Ono, Érika; Lafer, Manuel Mindlin; Weckx, Lily Yin; Granato, Celso Francisco Hernandes; Moraes-Pinto, Maria Isabel de

doi:10.1590/s0036-46652004000300008

Cited by 13 publications

(8 citation statements)

References 17 publications

(16 reference statements)

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“…18 Varicella IgG antibodies were measured by indirect ELISA, as previously described. 19 Seropositivity was considered if individuals had varicella antibodies equal to or greater than 0.1 IU/mL. 20…”

Section: Detection Of Serum Antibodies and Specific Antigensmentioning

confidence: 99%

Adherence and immune response to revaccination following hematopoietic stem cell transplantation at a pediatric onco‐hematology reference center

Gouveia-Alves

Gouveia

Ginani

et al. 2018

Transplant Infectious Dis

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Section: Detection Of Serum Antibodies and Specific Antigensmentioning

confidence: 99%

Adherence and immune response to revaccination following hematopoietic stem cell transplantation at a pediatric onco‐hematology reference center

Gouveia-Alves

Gouveia

Ginani

et al. 2018

Transplant Infectious Dis

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“…It is therefore unlikely that the antibody-antigen binding would be affected by a potential unfolding. 8M urea has also been used successfully to disrupt antigen-antibody binding without causing irreversible changes to the antigen in other avidity assays (Hedman and Seppala 1988;Blackburn, Besselaar et al 1991;Gray 1995;Ono, Lafer et al 2004;Smolander, Koskinen et al 2010). Furthermore, we have recently done a Biacore study were we measured the antigen-antibody binding capacity of purified IgG from anti-Yo positive patients to purified recombinant CDR2 (manuscript in preparation).…”

Section: Resultsmentioning

confidence: 99%

Antibody to CCDC104 is associated with a paraneoplastic antibody to CDR2 (anti-Yo)

Totland

Bredholt

Haugen

et al. 2009

Cancer Immunol Immunother

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Patients with cancer may develop paraneoplastic neurological syndromes (PNS) in which onconeural antibodies are important diagnostic findings. As the functional role of onconeural antibodies is largely unknown, insight gained by identifying associated antibodies may help to clarify the pathogenesis of the PNS. In this study, we identified patients with Yo antibodies who also had antibodies to an uncharacterized protein called coiled-coil domain-containing protein 104 (CCDC104). We found a significant association between CCDC104 and Yo antibodies (4 of 38, 10.5%), but not other onconeural antibodies (0 of 158) (P = 0.007, Fisher's exact test). The prevalence of CCDC104 antibodies was approximately similar in patients with cancer (8 of 756, 1.1%) and in healthy blood donors (2 of 300, 0.7%). CCDC104 antibodies were not associated with PNS, as this was found in only two of the ten CCDC104-positive patients. The CCDC104 protein, whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm. The association between Yo and CCDC104 antibodies may indicate functional similarities.

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“…For simple in-house preparations, the indirect ELISA is a good choice and also provides high sensitivity and flexibility [48,49]. The limitations with this process include possibility of high background signal due to the binding of all proteins to the wells of ELISA plates and non-specific binding of the secondary antibody [50].…”

Section: Discussionmentioning

confidence: 99%

Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63

El-Duah

Meyer

Sylverken

et al. 2019

Viruses

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Known human coronaviruses are believed to have originated in animals and made use of intermediate hosts for transmission to humans. The intermediate hosts of most of the human coronaviruses are known, but not for HCoV-NL63. This study aims to assess the possible role of some major domestic livestock species as intermediate hosts of HCoV-NL63. We developed a testing algorithm for high throughput screening of livestock sera with ELISA and confirmation with recombinant immunofluorescence assay testing for antibodies against HCoV-NL63 in livestock. Optimization of the ELISA showed a capability of the assay to significantly distinguish HCoV-NL63 from HCoV-229E (U = 27.50, p < 0.001) and HCoV-OC43 (U = 55.50, p < 0.001) in coronavirus-characterized sera. Evaluation of the assay with collected human samples showed no significant difference in mean optical density values of immunofluorescence-classified HCoV-NL63-positive and HCoV-NL63-negative samples (F (1, 215) = 0.437, p = 0.509). All the top 5% (n = 8) most reactive human samples tested by ELISA were HCoV-NL63 positive by immunofluorescence testing. In comparison, only a proportion (84%, n = 42) of the top 25% were positive by immunofluorescence testing, indicating an increased probability of the highly ELISA reactive samples testing positive by the immunofluorescence assay. None of the top 5% most ELISA reactive livestock samples were positive for HCoV-NL63-related viruses by immunofluorescence confirmation. Ghanaian domestic livestock are not likely intermediate hosts of HCoV-NL63-related coronaviruses.

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A simple and cheaper in house varicella zoster virus antibody indirect ELISA

Cited by 13 publications

References 17 publications

Adherence and immune response to revaccination following hematopoietic stem cell transplantation at a pediatric onco‐hematology reference center

Adherence and immune response to revaccination following hematopoietic stem cell transplantation at a pediatric onco‐hematology reference center

Antibody to CCDC104 is associated with a paraneoplastic antibody to CDR2 (anti-Yo)

Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63

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