Human papillomavirus genotypes in women with cervical cytological abnormalities from an area with high incidence of cervical cancer

Deluca, Gerardo Daniel; Lucero, Raúl Horacio; Civetta, M.T Martin De; Vicente, Lilian; Gorodner, Ofelia L.Z. de; Schelover, Eduardo; Alonso, Justo

doi:10.1590/s0036-46652004000100002

Cited by 13 publications

(13 citation statements)

References 20 publications

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“…Similarly, HR-HPV detection (65%) was within the range previously reported (30-73%) for the same population using the same detection method 13,29 .…”

Section: Discussionsupporting

confidence: 86%

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Circulation of human papillomavirus (HPV) genotypes in women from Córdoba, Argentina, with squamous intraepithelial lesions

Venezuela

Kiguen

Frutos

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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SUMMARYHuman papillomavirus (HPV) can induce a wide spectrum of squamous intraepithelial lesions (SIL) of varying severity. The aim of the present study was to establish the frequency of HPV infection and identify the genotypes circulating in women from Córdoba, Argentina, in relation to age and cytology. A total of 186 women, between 18 and 65 years old, with antecedents of SIL, underwent a pelvic examination and had cervical cells collected for cytology and HPV DNA detection. Ninety-six samples (51.6%) were positive for HPV detection, and sixty-three (65.6%) of them showed the presence of at least one HR-HPV. Low-and high-grade SIL showed significant association in patients younger than 35 years of age. We found 18 different genotypes, with a greater presence of HR-HPV. Genotypes 16 and 6 were the most frequent. Seven (7.3%) multiple infections, 85.7% of which had at least one HR-HPV, were detected. The detection of a large number of different HPV genotypes is a warning sign. It is thus necessary to strengthen the monitoring of the circulation of high-risk genotypes, currently less prevalent in intraepithelial lesions, as a control measure for the possible impact of the implementation of vaccines against genotypes 16 and 18.

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“…Similarly, HR-HPV detection (65%) was within the range previously reported (30-73%) for the same population using the same detection method 13,29 .…”

Section: Discussionsupporting

confidence: 86%

“…Some prevalence studies have indicated that the DNA of HPV 52 is present in approximately 2% of all cases of cervical squamous intraepithelial lesions 28,29 and that the presence of HPV 58 for these lesions ranges from 6% to 20%, which is the range within which these genotypes were detected in our work 13,24,25,26 .…”

Section: Discussionsupporting

confidence: 58%

Circulation of human papillomavirus (HPV) genotypes in women from Córdoba, Argentina, with squamous intraepithelial lesions

Venezuela

Kiguen

Frutos

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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“…Following infection by HPV, oncoproteins from the virus integrate with the tumor suppressor proteins p53 and pRb, which in turn alter the function of oncoproteins, thereby resulting in uncontrolled transcription activity, abnormal DNA replication and cell division, hence leading to tumor formation. 38 Our results showed that the women with HPV infection had an elevated frequency of micronuclei in relation to the control group. In our study, HPV status was compared with a variety of risk factors for cervical cancer and the results were concordant with the literature.…”

Section: Discussionmentioning

confidence: 50%

Prevalence of micronuclei in exfoliated uterine cervical cells from patients with risk factors for cervical cancer

et al. 2008

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CONTEXT AND OBJECTIVE: Pap smears are the most common and inexpensive screening method for cervical cancer. We analyzed micronucleus prevalence in exfoliated cervical mucosa cells, to investigate associations between increased numbers of micronuclei and risk factors for cervical cancer. DESIGN AND SETTING: Analytical cross-sectional study, at Instituto de Pesquisa em Oncologia (IPON). METHODS: Exfoliated cervical cells were obtained from 101 patients between September 2004 and November 2005. Patients' ages, habits (passive or active smoking, alcoholism and numbers of sexual partners), age at first sexual intercourse, contraceptive methods used, histories of sexually transmitted diseases, use of hormone replacement therapy, numbers of pregnancies and abortions, inflammatory cytology and cervical intraepithelial neoplasia (CIN) were obtained. Cells were collected using Ayre spatulas, transferred to vials containing 0.9% saline solution for micronucleus tests and analyzed at 1000x magnification. The number of micronuclei in 1,000 epithelial cells per patient sample was counted. RESULTS: Comparisons between groups with active (7.9 ± 7.8) and passive (7.2 ± 10.6) smoking versus no smoking (3.7 ± 5.1); with/without alcoholism (7.8 ± 1.4 and 6.9 ± 10.1); with/without inflammatory cytology (10.7 ± 10.5 and 1.3 ± 1.7); and with CIN I, II and III and no CIN (respectively 4.3 ± 4.3, 10.6 ± 5.3, 22.7 ± 11.9 and 1.3 ± 1.4) found elevated micronucleus prevalence (P < 0.05). CONCLUSIONS: We concluded that the prevalence of micronuclei in exfoliated uterine cervical cells was greater in patients with one or more risk factors for uterine cervical cancer than in patients without risk factors.

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“…Corroborando com esses achados, Câmara et al 20 , em Brasília, e De Luca et al 21 25 encontraram HPV de tipo 16 em 60,4% das mulheres com câncer cervical, em hospital de referência, em Belém/PA, utilizando técnica de PCR em material colhido por biópsia dirigida por colposcopia. Convém observar que esses autores estudaram a frequência de HPV 16 em grupos de mulheres com lesões mais graves, enquanto o trabalho ora apresentado considera todas as anormalidades citopatológicas, na sua análise, das quais somente 12 se apresentavam com HSIL ou lesão maior.…”

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PaPilomavírus Humano (HPv) em mulHeres submetidas a rastreamento Para CânCer de CérviCe uterina, belém – Pará – brasil

Noronha¹

2011

J Bras Doenças Sex Transm

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Introduction: human papillomavirus (HPV) is the agent of cervical uterine cancer. Objective: determine the prevalence of genital infection due to HPV and their correlation with results in the oncotic cytology. Methods: in a cross-sectional study, 1021 women, age 30 to 45 years were enrolled and submitted to cervical cancer screening. All patients answered a standard protocol. Samples of the uterine cervix were sent to citopathological analysis and to identification of HPV. Results: prevalence of HPV was 12.4%; in women with normal cervical cytology the prevalence of HPV was 8,7% compared to 43,4% in women with altered cervical cytology (28.9% among women with ASCUS/AGUS; 60.0% among women with LSIL; 90.0% among women with HSIL and 100.0% both in women with invasive carcinoma and in situ adenocarcinoma). Chance of detection of HSIL was 94 times higher in women who had HPV in the uterine cervix. A separate analysis including only the 116 HPV infected women revealed that HPV oncogenic types corresponded to 79.3% of the cases (71.8% in women with negative cervical cytology and 91.1% in women with altered cervical cytology). The frequency of oncogenic HPV types in 116 women infected with HPV was 79.3% [71.8% in women with negative citology and 91.1% in women with abnormalities in the citology exam). The odds to detect abnormalities in the citopatology exam was four times greater in the presence of HPV of high risk. HPV 16 was the most frequent type observed (24.4% of women with abnormal cytology and in 7,0% of those with negative cytology). Multiinfection was detected in 5.7% of women with abnormal cytology and in 1.1% with negative cytology. Conclusion: in this study, HPV infection and abnormal cytological findings in the uterine cervix were frequently observed and there was an association between them.

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Human papillomavirus genotypes in women with cervical cytological abnormalities from an area with high incidence of cervical cancer

Cited by 13 publications

References 20 publications

Circulation of human papillomavirus (HPV) genotypes in women from Córdoba, Argentina, with squamous intraepithelial lesions

Circulation of human papillomavirus (HPV) genotypes in women from Córdoba, Argentina, with squamous intraepithelial lesions

Prevalence of micronuclei in exfoliated uterine cervical cells from patients with risk factors for cervical cancer

PaPilomavírus Humano (HPv) em mulHeres submetidas a rastreamento Para CânCer de CérviCe uterina, belém – Pará – brasil

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