Intranasal sensitization with Blomia Tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum ige and peripheral blood eosinophil counts

Takeda, Fumiko; Arakawa, Takeshi; Toma, Hiromu; Ishii, Akira; Sato, Yoshiya

doi:10.1590/s0036-46652004000100001

Cited by 8 publications

(6 citation statements)

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“…This finding corroborated the work of Takeda and collaborators [15], who found an increase of total IgE in Bt E- and cholera toxin- sensitized mice. Dust mite proteins, such as Blo t 11, a paramyosin from B. tropicalis that is homologue to a helminth molecule, is responsible for the cross-reactivity found between helminths and dust-mite species [33], and may be leading to the non-specific IgE stimulation in Bt E-sensitized mice found in this and in the above mentioned work.…”

Section: Discussionsupporting

confidence: 92%

“…This is accordance to Takeda and collaborators' observation that the intranasal instillation alone of Bt E elicited an IgE antibody response only when the antigen was continuously administered for a period of over 24 weeks [15]. Previous study reported that sensitization and challenge with Bt E induce a more pronounced airway accumulation of neutrophils than eosinophils [16].…”

Section: Discussionsupporting

confidence: 89%

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Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

et al. 2010

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BackgroundThe dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Although a mouse model for B. tropicalis extract (BtE)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal, the molecular composition of the allergen and the experimental protocol.ObjectivesThis work had two objectives. The first was to study the anti-B. tropicalis allergic responses in different mouse strains using a short-term model of respiratory allergy to BtE. This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. The second objective was to investigate whether the best responder mouse strain could be used in an experimental model of allergy employing relatively low BtE doses.MethodsGroups of mice of four different syngeneic strains were sensitized subcutaneously with 100 μg of BtE on days 0 and 7 and challenged four times intranasally, at days 8, 10, 12, and 14, with 10 μg of BtE. A/J mice, that were the best responders to BtE sensitization, were used to compare the B. tropicalis-specific asthma experimental model with the conventional experimental model of ovalbumin (OVA)-specific asthma. A/J mice were also sensitized with a lower dose of BtE.ResultsMice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. Finally, immunization with a relatively low BtE dose (10 μg per subcutaneous injection per mouse) was able to sensitize A/J mice, which were the best responders to high-dose BtE immunization, for the development of allergy-associated immune and lung inflammatory responses.ConclusionsThe described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

et al. 2010

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“…Aside from adsorption of total IgE, adsorptions of specific IgE, such as Dermatophagoides pteronyssinus (IgE D1 ) and Blomia tropicalis (IgE D201 ), were also taken into account. The allergic sensitization to the mites B. tropicalis and D. pteronyssinus is related to allergic respiratory diseases development in many countries, mostly tropical ones (12). The effects of operating variables (superficial velocity [ν], number of perfusions [np], ratio of plasma volume [pv] to bed volume [bv][pv/bv], and temperature [T]) on the capacity and selectivity of the system were investigated.…”

mentioning

confidence: 99%

In Vitro Evaluation of Biospecific and Pseudobiospecific Ligands Aimed at Extracorporeal Treatment for Immunoglobulin E Removal

Duarte¹,

Zollner

Bueno³

2006

Artificial Organs

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This work investigated the potential use of an alternative adsorbent to anti-immunoglobulin E (IgE)-agarose for IgE selective adsorption therapy. A screening of several commercially available adsorbents (Concanavalin A, Lens culinaris[Lc], d-tryptophan, poly-l-lysine, and aminohexyl immobilized on agarose) was done through batch system assays, considering some criteria, such as adsorption capacity, selectivity, and biocompatibility. In the Lc-agarose adsorbent, total IgE, and specific IgE--for the airborne allergens Dermatophagoides pteronyssinus and Blomia tropicalis--were significantly better removed (63, 58, and 59%, respectively) than immunoglobulin G (19%), immunoglobulin A (33%), immunoglobulin M (9%), and albumin (18%). This adsorbent was packed into a column and the effect of superficial velocity, ratio of plasma volume to bed volume, number of perfusions, and temperature on IgE adsorption were evaluated. In vitro simulation of therapeutic adsorption (single perfusion) indicated that about 50% of total IgE could be eliminated.

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“…delivery will also cause an immune reaction at the mucosal surface. This route consists of applying a small volume of treatment solution (~10 µl) into a mouse's nasal passage (Takeda et al 2004). Based on conclusions from Constant and colleagues, administering the antigen via the i.n.…”

mentioning

confidence: 99%

“…as a priming dose, and subsequent sensitization treatments administered LT-B and PE via food pellet. By using a strong adjuvant to trigger the initial immune response, later doses with a weaker adjuvant may induce isotype switching to IgE(Takeda et al 2004). The ability to shift the isotype antibody response could affect portions of the population which currently have an IgG immune response to various allergens or genotypes indicative of atopy…”

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confidence: 99%

Allergy models and related assays to test the allergic qualities of Escherichia coli heat labile toxin subunit B

Rahn

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Chapter 1: Literature Review I. Allergic Responses II. Animal Allergy Models III. Cholera Toxin and Heat Labile Toxin Chapter 2: Cholera Toxin or Escherichia coli Heat Labile Toxin Subunit B and Mouse Peanut Allergy Model I. Abstract II. Introduction III. Materials and Methods IV. Results V. Discussion Acknowledgements iii 1 1 5 16 32 32 33 35 45 55 92 iii Abstract Transgenic maize containing Escherichia coli heat labile toxin subunit B (LT-B) hasproven to be a strong mucosal immunogen in animal models and stimulates an IgG1 and IgA antibody response when administered orally. There is concern the T-helper cell 2 response stimulated by LT-B could sensitize the immune system to produce an allergic response. This concern is further compounded by the approximate 80% homology which exists between E. coli heat labile toxin and cholera toxin (CT), a known allergy inducer. Thus, the research was based on ascertaining what possible allergic stimulation, if any, LT-B could have on animal models.The first experiment was to reproduce an established allergy model as a positive control, using peanuts as the allergen and CT as the adjuvant. The animals were administered 10 µg CT and 5 mg of peanut extract via intragastric gavage per dose and received 4 sensitizing doses. A number of assays were also established to measure various aspects of an allergic response. Mice treated with CT and peanut extract showed higher concentrations of total and peanut specific IgE, peanut and CT specific IgG1, and higher visual scores of allergy symptoms. A second experiment was designed in which animals were fed food pellets containing LT-B (100 µg or 20 µg) with peanuts. One control group was fed CT and peanuts. Neither LT-B fed animal group produced an allergic response to peanut; however, the animals fed CT and peanuts also did not produce an allergic response.A third experiment was designed to optimize the parameters of the CT and peanut extract positive control, with CT and peanuts both being gavaged or fed at various concentrations (CT = 20, 10, or 0 µg; peanut = 20, 10, 5, or 0 mg). The route of delivery was the most important factor. Mice treated with CT and peanut both by intragastric gavage showed

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Intranasal sensitization with Blomia Tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum ige and peripheral blood eosinophil counts

Cited by 8 publications

References 18 publications

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

In Vitro Evaluation of Biospecific and Pseudobiospecific Ligands Aimed at Extracorporeal Treatment for Immunoglobulin E Removal

Allergy models and related assays to test the allergic qualities of Escherichia coli heat labile toxin subunit B

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