2003
DOI: 10.1590/s0036-46652003000700001
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Abstract: The recent history of Brazilian Parasitology and that of the Chagas' Disease Meeting are deeply interwoven. Actually, the "meeting" was one of the efforts to revive basic research in Parasitology, which was near total collapse in the early 1970s in Brazil. The "meeting", the visible arm of the Programa Integrado de Doenças Endêmicas do CNPq (PIDE), has done its job. In this presentation we intend to show the "meeting" links with the development of Parasitology and the success of this joint scientific venture q… Show more

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Cited by 10 publications
(11 citation statements)
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“…Details will be described elsewhere. 2 Parasites-T. cruzi epimastigotes of the Tulahuen strain were grown in liver infusion tryptose medium containing 10% fetal calf serum, at 28°C with continuous agitation (21). T. cruzi tissue culture forms of Berenice, Tulahuen, Y, and 22 strains were maintained in monolayers of L-6, HeLa, or NIH-3T3 cells grown in Dulbecco's minimal essential medium supplemented with 100 units/ml penicillin, 100 g/ml streptomycin, and 5% (v/v) fetal calf serum at 37°C in 5% CO 2 .…”
Section: Methodsmentioning
confidence: 99%
“…Details will be described elsewhere. 2 Parasites-T. cruzi epimastigotes of the Tulahuen strain were grown in liver infusion tryptose medium containing 10% fetal calf serum, at 28°C with continuous agitation (21). T. cruzi tissue culture forms of Berenice, Tulahuen, Y, and 22 strains were maintained in monolayers of L-6, HeLa, or NIH-3T3 cells grown in Dulbecco's minimal essential medium supplemented with 100 units/ml penicillin, 100 g/ml streptomycin, and 5% (v/v) fetal calf serum at 37°C in 5% CO 2 .…”
Section: Methodsmentioning
confidence: 99%
“…The protozoans were grown in liver-infusion tryptone broth-LIT 17) supplemented with 10% fetal bovine serum (Gibco Invitrogen Corporation, New York, U.S.A.).…”
Section: Plant Materialsmentioning
confidence: 99%
“…42 For the experiments, parasites obtained from 72-hour cultures were inoculated into fresh LIT medium and then added to 96-well plates at a concentration of 5 × 10 7 cells per well. To obtain cell-derived trypomastigote forms, Vero cell cultures were infected with trypomastigote forms at a 10:1 parasite:host cell ratio.…”
Section: Antitrypanosomal Bioassaymentioning
confidence: 99%