Diagnosis of pulmonary pneumocystosis by microscopy on wet mount preparations

Bava, Amadeo Javier; Cattáneo, Sylvia; Bellegarde, E

doi:10.1590/s0036-46652002000500009

Cited by 10 publications

(12 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A study of 389 children at risk for or infected with HIV-1 conducted from February 1990 to June 1997 found that severe bacterial infection and PcP were the most common AIDS-defining conditions and death-related diseases among AIDS patients (Fallo et al , 2002). Another study demonstrated a high rate of PcP (35%) among AIDS patients in intensive care units (Bava et al , 2002). Although the comparison of the pre and post-HAART era in Argentine AIDS patients revealed an increased PcP rates from 5.9% to 9.4%, TB was the main cause of hospital admission for HIV patients in both study periods (Pérez et al , 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pneumocystis jiroveciipneumonia in developing countries

et al. 2011

View full text Add to dashboard Cite

Pneumocystis pneumonia (PcP) is a serious fungal infection among immunocompromised patients. In developed countries, the epidemiology and clinical spectrum of PcP have been clearly defined and well documented. However, in most developing countries, relatively little is known about the prevalence of pneumocystosis. Several articles covering African, Asian and American countries were reviewed in the present study. PcP was identified as a frequent opportunistic infection in AIDS patients from different geographic regions. A trend to an increasing rate of PcP was apparent in developing countries from 2002 to 2010.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Also, investigations have been conducted in Argentina and Peru (Bava et al , 2002; Eza et al , 2006). …”

Section: Introductionmentioning

confidence: 99%

Pneumocystis jiroveciipneumonia in developing countries

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The sample was centrifuged (1 500 rpm/15´), and fresh microscopy, smears stained with Kinyoun [4] , Giemsa (Figure 1) and a rapid modification of the Grocott technique [5] were performed. Also, in our center the number of pulmonary cryptococcosis diagnosed in AIDS patients is significantly lower than expected as compared with the cases of meningoencephalitis which is produced by the infection of Cryptococcus neoformans (C. neoformans) through inhalation of the propagules [6] . Part of the respiratory secretions was processed in the microbacteriology, mycology and bacteriology services of the Muñiz Hospital and the result was negative.…”

Section: Case Reportmentioning

confidence: 67%

“…Part of the respiratory secretions was processed in the microbacteriology, mycology and bacteriology services of the Muñiz Hospital and the result was negative. The wet smear revealed numerous leukocytes, filariform and rhabditiform larvae of S. stercoralis and foamy exudates [6] . A quick modification of the Grocott technique to the smears showed P. jiroveci cysts, while the Kinyoun stain was negative for acid resistant bacteria and protozoa.…”

Section: Case Reportmentioning

confidence: 99%

A case report of pulmonary coinfection of Strongyloides stercoralis and Pneumocystis jiroveci

Bava¹,

Romero²,

Prieto³

et al. 2011

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

A case of pulmonary coinfection by Strongyloides stercoralis and Pneumocystis jiroveci has been detected in an AIDS patient treated in the Respiratory Intensive Care Unit of the Muñiz Hospital. At diagnosis, the patient presented cough with mucopurulent expectoration, dyspnea, fever, bilateral pulmonary infiltrates on the chest X-ray, negative bacilloscopy for acid fast bacteria and a CD4(+) T lymphocytes count of 52 cells/µL. The microbiological diagnosis was achieved by microscopic observation of the respiratory secretions obtained by bronchoalveolar lavage, while the wet mount examination revealed rhabditiform and filariform larvae of the nematode and foamy exudates, pathognomonic of the pulmonary pneumocystosis. It was the unique case of this association among about 3 000 samples performed in our laboratory in the last 10 years and diagnosed by microscopy. Other complementary stains (a rapid modification of Grocott, Kinyoun and Giemsa) were applied to the smears after the diagnosis of mycotic and parasitary infections achieved by fresh microscopy. Both physicians and microbiologists should take into account the possible coexistence of respiratory pathogens in immunocompromised patients, such as those with AIDS.

show abstract

“…[14] However, The reported incidence of PcP is between 32% and 45% in the current literature. [8151617]…”

Section: Discussionmentioning

confidence: 99%

Profile of pneumocystis infection in a tertiary care institute in North India

Kaur

Dewan

2016

Indian J Sex Transm Dis

View full text Add to dashboard Cite

Context:Pneumocystis jirovecii pneumonia (PcP) is still remains a common opportunistic disease in human immunodeficiency virus (HIV) infected individuals. Study on PcP in developing countries are scarce.Aims:To study the occurrence of P. jirovecii infection in clinically suspected individuals in a tertiary care institute.Settings and Design:Retrospective study conducted in a tertiary care hospital.Materials and Methods:Two years data regarding respiratory sample analysis, HIV status, and cluster of differentiation 4 (CD4) cell count of clinically suspected pneumocystis infection patients with known/unknown HIV status were analyzed.Results:Data of 45 eligible patients were analyzed. The majority of the patients were male (between 21 and 50 years of age). Total 26 (57.7%) patients were HIV reactive, of which 14 had CD4 count of <200 cells/mm3. 20 patients (9 HIV reactive and 11 unknown HIV status) were confirmed with pneumocystosis by direct fluorescent antibody (DFA) staining. Four of 14 HIV reactive individuals who had CD4 count of <200 cells/mm3 and 5 of 12 HIV reactive individuals who had CD4 count of >200 cells/mm3 were positive for pneumocystosis.Conclusions:Pneumocystis pneumonia is still prevalent in North India and is mainly affecting patients in economically productive and sexually active age group. To diagnose pneumocystosis, DFA is an easily available method in resource-limited settings. Appreciating the actual HIV or immunodeficiency status and the CD4 profile of an individual with symptoms of pneumocystis infection will help the clinicians in early diagnosis and initiation appropriate therapy in individuals living with the disease.

show abstract

Diagnosis of pulmonary pneumocystosis by microscopy on wet mount preparations

Cited by 10 publications

References 2 publications

Pneumocystis jiroveciipneumonia in developing countries

Pneumocystis jiroveciipneumonia in developing countries

A case report of pulmonary coinfection of Strongyloides stercoralis and Pneumocystis jiroveci

Profile of pneumocystis infection in a tertiary care institute in North India

Contact Info

Product

Resources

About