Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil

Ávila-Campos, Mário Júlio; Velásquez-Meléndez, Gustavo

doi:10.1590/s0036-46652002000100001

Cited by 40 publications

(36 citation statements)

References 14 publications

(18 reference statements)

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“…Ltd., SP, Brazil), erythromycin and tetracycline (Extratus Farm., Pr, Brazil), metronidazole (Alquimia Farm., SP, Brazil), and penicillin V (Wyeth-Whitehall Ltd., SP, Brazil). The metals were as follows: lead chloride (9PbCl 2 ) and cadmium sulfate (3CdSO 4 …”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

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Prevotella intermedia and Porphyromonas gingivalis isolated from osseointegrated dental implants: colonization and antimicrobial susceptibility

Pfau¹,

Ávila-Campos²

2005

Braz. J. Microbiol.

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The colonization and antimicrobial susceptibility of P. intermedia and P. gingivalis isolated from peri-implant and gingival sulcus samples were determined. Samples were collected from 30 patients submitted to implant in three different times: at the moment of the surgery, 20 and 60 days after the implant installation. Organisms were identified by using a biochemical tests or API 32-A kit and by PCR. The antimicrobial susceptibility was determined by using an agar dilution method. Nineteen P. intermedia (4 from peri-implant sites and 15 from gingival sulcus), and only seven P. gingivalis from gingival sulcus were isolated. Organisms were detected by PCR from seven peri-implant and 32 gingival samples. Bacteria were susceptible to the used antibiotics except to azithromycin with 65% of resistance for P. intermedia strains. Both tested species were susceptible to cadmium, nickel and palladium, and showed different resistance rates to titanium, aluminum and mercuric chloride. Most of P. intermedia strains were resistant to lead, silver, copper, titanium, zinc, aluminum and mercuric chloride. Bacteria colonized implants after 60 days of surgery and PCR may be used as a tool for bacterial detection in implantology.

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Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

“…A polymerase chain reaction (PCR) has been used for the direct detection of periodontal pathogens from subgingival clinical samples (4). However, detection of periodontal organisms in Brazilian dentistry is still not a routine.…”

Section: Introductionmentioning

confidence: 99%

Prevotella intermedia and Porphyromonas gingivalis isolated from osseointegrated dental implants: colonization and antimicrobial susceptibility

Pfau¹,

Ávila-Campos²

2005

Braz. J. Microbiol.

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“…Moreover, it has been demonstrated a strong correlation between the presence of putative periodontal organisms and the destruction of periodontal tissues. Organisms such as Porphyromonas gingivalis, Prevotella intermedia, Actinobacillus actinomycetemcomitans, Treponema denticola, Fusobacterium nucleatum, Dialister pneumosintes, Tannerella forsythensis, Treponema denticola, Eikenella corrodens and Campylobacter rectus have been related to several forms of periodontal disease in humans and animals (2,3,5,29).…”

Section: Introductionmentioning

confidence: 99%

“…Molecular methods are currently available for detecting periodontopathogens, since techniques of bacterial culture are used to identify putative anaerobic pathogens from destructive periodontal disease, but it can be somewhat cumbersome and expensive (5). Polymerase chain reaction (PCR) has been used in the direct identification of periodontal pathogens from subgingival specimens due to ability to accurately detect microbial species in mixed populations (3,4).…”

Section: Introductionmentioning

confidence: 99%

Detection of putative periodontal pathogens in subgingival specimens of dogs

Nishiyama¹,

Senhorinho²,

Gioso³

et al. 2007

Braz. J. Microbiol.

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In this study, the presence of putative periodontal organisms, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Fusobacterium nucleatum, Dialister pneumosintes, Actinobacillus actinomycetemcomitans, Campylobacter rectus, Eikenella corrodens and Treponema denticola were examined from subgingival samples of 40 dogs of different breeds with (25) and without (15) periodontitis, by using the PCR method. The PCR products of each species showed specific amplicons. Of the 25 dogs with periodontitis, P. gingivalis was detected in 16 (64%) samples, C. rectus in 9 (36%), A. actinomycetemcomitans in 6 (24%), P. intermedia in 5 (20%), T. forsythensis in 5 (20%), F. nucleatum in 4 (16%) and E. corrodens in 3 (12%). T. denticola and D. pneumosintes were not detected in clinical samples from dogs with periodontitis. Moreover, P. gingivalis was detected only in one (6.66%) crossbred dog without periodontitis. Our results show that these microorganisms are present in periodontal microbiota of dogs with periodontitits, and it is important to evaluate the role of these putative periodontal microorganisms play in the periodontitis in household pets particularly, dogs in ecologic and therapeutic terms, since these animals might acquire these periodontopahogens from their respective owners.

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“…These characteristics have a relationship with both the prevalence of periodontal diseases and the pathogens related to them (9)(10)(11)(12)(13)(14)(15). Furthermore, studies about the transmission of periodontopathogens are valuable to elucidate its significance as a risk factor for the establishment of periodontal diseases and to search available ways for their prevention (16).…”

Section: Introductionmentioning

confidence: 99%

Transmission of Aggregatibacter actinomycetemcomitans between Brazilian women with severe chronic periodontitis and their children

et al. 2007

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This study evaluated the transmission of Aggregatibacter actinomycetemcomitans (Aa) in women with severe chronic periodontitis and their children. Thirty women (mean age = 36.1±6.0 years) who were mothers of at least one child aged 7 to 16 years were enrolled. In order to investigate mother-child transmission of Aa, the children were also evaluated when their mothers were colonized by the bacterium. Subgingival plaque samples of each woman were collected from 3 sites (mean probing depth of 7.3±1.2 mm and mean clinical attachment level of 7.9±1.5 mm) and pooled in reduced transport fluid (RTF). These samples were processed, inoculated onto TSBVagar selective medium and incubated at 37°C in microaerophilic atmosphere for 5 days. Aa was identified on the basis of colony morphology, Gram staining, catalase and oxidase reactions. Aa was found in 8 out of 30 women. Therefore, 8 children from these women (mean age= 12 ± 3.7 years) were evaluated, but Aa was found only in 2 of them. Aa strains of the two mother-child pairs were evaluated by arbitrarily-primed polymerase chain reaction (AP-PCR), although it was not found similarity between the amplitypes of each pair. No Aa transmission was found between Brazilian women with severe chronic periodontitis and their children.

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Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in São Paulo, SP, Brazil

Cited by 40 publications

References 14 publications

Prevotella intermedia and Porphyromonas gingivalis isolated from osseointegrated dental implants: colonization and antimicrobial susceptibility

Prevotella intermedia and Porphyromonas gingivalis isolated from osseointegrated dental implants: colonization and antimicrobial susceptibility

Detection of putative periodontal pathogens in subgingival specimens of dogs

Transmission of Aggregatibacter actinomycetemcomitans between Brazilian women with severe chronic periodontitis and their children

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