Validation of molecular approaches for the diagnosis of American cutaneous leishmaniasis in Pernambuco State

Rodrigues, Eduardo Henrique Gomes

doi:10.1590/s0036-46652000000600008

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Martins et al (2010) also demonstrated the efficiency of PCR carried out using peripheral venous blood samples for detection of L. braziliensis DNA in humans with no cutaneous lesions. According to Rodrigues (2000), this method displays significantly higher sensitivity and specificity than direct testing techniques, isolation from culture, and IFA. These attributes favor the identification of subclinical cases with low parasitism rates, enabling better monitoring of treatment and more accurate assessment of disease prevalence in endemic areas (Rodrigues, 2000).…”

Section: Discussionmentioning

confidence: 99%

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Leishmaniasis is a disease caused by protozoa of the genus Leishmania. Two distinct forms are recognized: visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). In the Americas, the causative agent of VL is L. infantum chagasi, whereas L. braziliensis is principally responsible for CL. Domestic dogs constitute the main source of VL in urban environments, and have also been implicated in CL epidemiology. We carried out molecular and serological surveys to detect Leishmania infection in dogs from the municipality of Ituberá in Bahia, Brazil. Furthermore, we identified risk factors associated with illness in dogs from this locality. Blood samples were collected from 399 dogs and tested using an indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) to detect Leishmania spp antibodies and L. infantum chagasi and L. braziliensis DNA, respectively. Dogs were clinically evaluated and tissue samples from those exhibiting skin lesions were examined for parasites. In addition, the dog owners completed an epidemiological questionnaire to identify factors associated with infection. Skin lesions consistent with CL were found on 37 (9.3%) of the evaluated animals, but parasitological examination was negative for all samples. The IFA returned positive results for 60 (15%) dogs. PCR identified DNA from L. braziliensis in 86 (21.6%) animals, where as all samples proved negative for L. infantum chagasi. The 134 dogs (33.6%) testing positive using IFA and/ or PCR were considered infected, and of these, only 13 demonstrated skin lesions. Animals from rural areas were 3.39-times more likely to be infected compared to those in urban environments.

show abstract

Section: Discussionmentioning

confidence: 99%

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

et al. 2016

View full text Add to dashboard Cite

show abstract

American Tegumentary Leishmaniasis and Flow Cytometry: A Review

BC¹,

APO²

2016

J Med Microb Diagn

View full text Add to dashboard Cite

American Tegumentary Leishmaniasis (ATL) is a parasitic disease caused by protozoans of the genus Leishmania and transmitted by the bite of sandflies. It is considered neglected due to its high incidence and morbidity, mainly in developing countries. The treatment for this disease besides expensive, is toxic and has limitations such as resistance, both from the parasite and the host; and invasiveness, making it not quite acceptable for some patients. There is still no available vaccine for the disease, what makes the correct diagnosis the better alternative to guide the treatment and therefore manage it. The diagnosis of suspected cases has to gather epidemiological, clinical and laboratorial data, since the disease can be misdiagnosed with other dermatological conditions. Immunological methods are commonly used for detecting and monitoring diseases, and the main techniques used in the routine for ATL are the Montenegro Skin Test, Enzyme-linked Immunosorbent Assay, Immunofluorescence Assay and Western Blot. Although these approaches have good sensitivity levels, they lack on specificity and have some limitations like crossed-reactions with other diseases caused by trypanosomatid parasites. One different serological approach, which is becoming an alternative method for the diagnosis of this disease, is Flow Cytometry; it has shown balanced levels of specificity and sensitivity and also a better accuracy when compared to other methods used in routine. Therefore, this article shows the recent advances on the diagnosis of ATL by flow cytometry and by using the findings on the literature, aims to guide researchers on what should be the focus to have a better diagnostic method for this disease.

show abstract

Validation of molecular approaches for the diagnosis of American cutaneous leishmaniasis in Pernambuco State

Abstract: Gomes-Validação de abordagens moleculares para o diagnóstico da leishmaniose tegumentar americana em Pernambuco. Recife, 2000.

Cited by 2 publications

References 0 publications

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

American Tegumentary Leishmaniasis and Flow Cytometry: A Review

Contact Info

Product

Resources

About