Gamma 60Co DL50/30 of Biomphalaria glabrata (Say, 1818)

Abstract

The variation of resistance to (60)Co gamma-rays of Biomphalaria glabrata was studied. A population of 480 mollusks was observed during 30 days - distributed in 8 groups of snails isolated and 8 groups of snails in colonies - after exposure (30 snails per group per dose) to increasing doses of gamma radiation. Doses of 10, 20, 40, 60, 80, 160, 320 and 640 Gy from a Gamma-cell (60)Co irradiator, were applied to the test groups and two groups control (non-irradiated) of snails - isolated and colony - were kept apart. After have been exposed, the snails were drew back to the aquaria where they were maintained before. The survival was estimated on a daily score of the alive animals in each group-dose, starting after the irradiation exposure day. As a result, the survival self-fertilization forms (DL(50/30) = 218.2 Gy) was found greater than in cross-fecundation forms. These data point to a low radio-resistance on the cross-fertilization forms - the sexual reproductive form - which is most found in nature. The lower radio-resistance of the cross-fertilization forms suggests the presence of some sex-linked hormonal factor related to this phenomenon.

Oxidative stress in mollusks Biomphalaria glabrata exposed to gamma radiation

“…The mollusks were irradiated, based on the work carried out by Carvalho and colleagues [14], at doses of 10, 15, 20 and 25 Gy in the 60 Co Gammacell® source (dose rate of 3.532 kGy/h) from the Department of Nuclear Energy of the Universidade Federal de Pernambuco. The control group was kept under the same conditions as the others, besides not being exposed to ionizing radiation.…”