Detection and identification of dengue-2 virus from Santa Cruz-Bolivia by a single tube RT-PCR method

Peredo, Carlos Herrejón; Garrón, Tania; Pelegrino, José Luis; Harris, Eva; Gianella, Alberto

doi:10.1590/s0036-46651999000300015

Cited by 3 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Teams of scientists from CDC and the Instituto de Medicina Tropical "Pedro Kouri," Havana, Cuba, collaborated with Nicaraguan investigators, leading to the discovery that the etiological agent was in fact Leptospira (3). In August 1997, Bolivian scientists at the Centro Nacional de Enfermedades Tropicales used this RT-PCR assay to type dengue virus in Bolivia for the first time and to identify dengue-2 as the serotype responsible for the 1997 epidemic in Santa Cruz, corroborating the results reported by CDC (27). Thus, in-country access to simplified PCRbased techniques is useful for immediate public health purposes as well as for long-term epidemiological studies.…”

Section: Fig 4 (A) Detection Of Dengue Virus In Pools Of Mosquitoessupporting

confidence: 56%

“…The utility of this single-step assay has been demonstrated in laboratories in developing countries, including Nicaragua (Fig. 3), Bolivia (27), Ecuador (21), and Guatemala (31). Prior to the introduction of the simplified dengue virus RT-PCR assay, the National Virology Laboratory at the Nicaraguan Ministry of Health had been unable to type circulating dengue viruses due to the lack of the cell culture facilities or mosquito colonies necessary for classical viral isolation procedures.…”

Section: Discussionmentioning

confidence: 99%

“…Figure 3B shows that dengue-3 was circulating in two different regions of the country (lanes 3 and 5, Bluefields; lane 12, Chontales). This assay has been used for routine epidemiological surveillance in Nicaragua since 1995 and has been implemented by collaborators at the Centro Nacional de Enfermedades Tropicales in Santa Cruz, Bolivia (27). The RT-PCR assay with rTth has also been successfully used to analyze RNA extracted from clinical specimens (data not shown).…”

Section: Single-tube Rt-pcr Assaymentioning

confidence: 99%

See 2 more Smart Citations

Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR

et al. 1998

Self Cite

View full text Add to dashboard Cite

In recent years, dengue viruses (serotypes 1 to 4) have spread throughout tropical regions worldwide. In many places, multiple dengue virus serotypes are circulating concurrently, which may increase the risk for the more severe form of the disease, dengue hemorrhagic fever. For the control and prevention of dengue fever, it is important to rapidly detect and type the virus in clinical samples and mosquitoes. Assays based on reverse transcriptase (RT) PCR (RT-PCR) amplification of dengue viral RNA can offer a rapid, sensitive, and specific approach to the typing of dengue viruses. We have reduced a two-step nested RT-PCR protocol to a single-tube reaction with sensitivity equivalent to that of the two-step protocol (1 to 50 PFU) in order to maximize simplicity and minimize the risk of sample cross-contamination. This assay was also optimized for use with a thermostable RT-polymerase. We designed a plasmid-based internal control that produces a uniquely sized product and can be used to control for both reverse transcription or amplification steps without the risk of generating false-positive results. This single-tube RT-PCR procedure was used to type dengue viruses during the 1995 and 1997-1998 outbreaks in Nicaragua. In addition, an extraction procedure that permits the sensitive detection of viral RNA in pools of up to 50 mosquitoes without PCR inhibition or RNA degradation was developed. This assay should serve as a practical tool for use in countries where dengue fever is endemic, in conjunction with classical methods for surveillance and epidemiology of dengue viruses.

show abstract

Section: Fig 4 (A) Detection Of Dengue Virus In Pools Of Mosquitoessupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Single-tube Rt-pcr Assaymentioning

confidence: 99%

See 1 more Smart Citation

Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR

et al. 1998

Self Cite

View full text Add to dashboard Cite

show abstract

“…In Bolivia, dengue fever was mentioned for the first time in 1931 [ 3 ], but well-documented cases during the 1990s appeared with the isolation of DEN-1 and DEN-2 viruses [ 4 , 5 , 6 ], followed by DEN-3 at the beginning of the 2000s [ 3 ]. Supported by the Rockefeller Foundation, the A. aegypti eradication was completed in 1948, but the mosquito reappeared during the 1980s [ 7 ] and quickly spread to all tropical parts of the country.…”

Section: Introductionmentioning

confidence: 99%

Evolution of Dengue Disease and Entomological Monitoring in Santa Cruz, Bolivia 2002 – 2008

et al. 2015

View full text Add to dashboard Cite

BackgroundIn the context of a rapid increase of dengue cases in the Americas, a monitoring system based on systematic serological control (IgM) of patients consulting for suspected dengue was developed in Bolivia at the end of the 1990s. In the most affected city of Santa Cruz, this system was complemented by an entomological surveillance program based on periodical search for immature stages of Aedes aegypti in dwelling water-holding containers. Here, we analyze these data and describe dengue patterns over 6 years (2002–2008), highlighting the spatial distribution of patients and vectors.Methodology /Principal FindingsData mining concerned six annual epidemic cycles (2002–2008), with continuous serological and clinical results and entomological data from 16 surveys, examined at the scales of 36 urban areas and four concentric areas covering the entire city. Annual incidence varied from 0.28‰ to 0.95‰; overall incidence was higher in women and adults, and dengue dynamics followed successive periods of high (January–June) and low (July–December) transmission. Lower numbers of cases from the city center to the periphery were observed, poorly related to the more homogeneous and permanent distribution of A. aegypti. "Plant pots" were a major vector source in the city center, and "Tires" and "Odds and ends" beyond the second ring of the city.Conclusions/SignificanceOver the years, the increasing trend of dengue cases has been highlighted as well as its widespread distribution over the entire city, but an underestimation of the number of cases is strongly suspected. Contrary to popular belief, the city center appears more affected than the periphery, and dengue is not particularly related to waste. Interestingly, the clinical diagnosis of dengue by physicians improved over the years, whatever the gender, age and residential area of suspected cases.

show abstract

Electrospun manganese (III) oxide nanofiber based electrochemical DNA-nanobiosensor for zeptomolar detection of dengue consensus primer

Tripathy

Vanjari

Singh

et al. 2017

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Detection and identification of dengue-2 virus from Santa Cruz-Bolivia by a single tube RT-PCR method

Cited by 3 publications

References 2 publications

Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR

Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR

Evolution of Dengue Disease and Entomological Monitoring in Santa Cruz, Bolivia 2002 – 2008

Electrospun manganese (III) oxide nanofiber based electrochemical DNA-nanobiosensor for zeptomolar detection of dengue consensus primer

Contact Info

Product

Resources

About