Simplified Diagnosis of Malaria Infection: GFM/PCR/Elisa a Simplified Nucleic Acid Amplification Technique by PCR/Elisa

Machado, Ricardo Luiz Dantas; Garret, Denise Oliveira; Adagu, I. S.; Warhurst, David C.; Póvoa, Marinete Marins

doi:10.1590/s0036-46651998000500014

Cited by 6 publications

(1 citation statement)

References 5 publications

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“…Em geral, possuem a mesma ou menor eficiência que a gota espessa (Murray, 2003;Belizario et al, 2005 (Machado et al, 1998, Zaqueri et al, 2002, Kanunfre, 2003. A sua utilização tem sido indicada, em áreas endêmicas, para a detecção de assintomáticos e triagem de doadores de sangue (Speers et al, 2003, Kitchen e Chiodini, 2006.…”

Section: Diagnóstico De Maláriaunclassified

Padronização de teste multiparamétrico para a pesquisa de anticorpos IgG anti-T.cruzi, anti-T.pallidum, anti-P. vivax e anti-P falciparum, empregando a técnica de Dot-ELISA

Coelho¹

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À Irene Soares, Elaine Lemos e Eufrozina Umesawa por cederem os antígenos utilizados neste trabalho. À Monica Ericó Kudó, pela amizade de longa data, por estar por perto nas horas difíceis e nas horas boas, pela solidariedade, pelo apoio dado por ela na época difícil de adaptação em São Paulo e pelos tantos "galhos quebrados". À Amanda Farage Frade, que junto com a Mônica, me apoiou e me acolheu em São Paulo. Sua amizade vai estar sempre comigo. À Dra. Sandra do Lago Moraes, pelo carinho, sabedoria e amizade, por ser uma pessoa especial e um exemplo a seguir... À Dra. Guita R. Elefant, pelos conselhos, pela amizade, pela boa vontade no nosso laboratório, companhia e apoio. À Márcia Otani e Joyce Cotomacio do Laboratório de Controle de Qualidade do Hemocentro de São Paulo, pela atenção, ajuda e dedicação demonstradas por elas e por me receberem sempre tão bem. À Dra. Silvia di Santi e funcionários da SUCEN que ajudaram na coleta do material dos pacientes com malária. Ao meu orientador Dr. Antônio Walter Ferreira pela oportunidade de realizar este trabalho. Às amigas do laboratório,

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Section: Diagnóstico De Maláriaunclassified

Padronização de teste multiparamétrico para a pesquisa de anticorpos IgG anti-T.cruzi, anti-T.pallidum, anti-P. vivax e anti-P falciparum, empregando a técnica de Dot-ELISA

Coelho¹

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Identification of Staphylococcus aureus enterotoxins A and B genes by PCR-ELISA

Gilligan

Shipley

Stiles

et al. 2000

Molecular and Cellular Probes

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Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment

Machado

Póvoa

2000

Transactions of the Royal Society of Tropical Medicine and Hygi

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The present study evaluated the glass fibre membrane (GFM)-polymerase chain reaction (PCR)-enzyme-linked immunosorbent assay (ELISA) technique for genotyping the Plasmodium vivax variants, to verify the distribution of P. vivax variants (VK210, VK247 and P. vivax-like) in parts of Brazil and their correlation with levels of parasitaemia, previous malaria experience and clearance of parasitaemia linked to different treatment schedules. The samples were taken from individuals living in Macapá, Porto Velho and Belém, all of which are endemic areas of vivax malaria in the Amazon region of Brazil. Blood samples were collected on GFMs. The gene that codes for the circumsporozoite proteins of P. vivax variants was amplified by PCR and the amplified fragments were hybridized to variant-specific, digoxigenin-labelled oligonucleotide probes by ELISA. The GFM-PCR-ELISA technique was shown to be accurate for epidemiological surveys of the vivax complex. All variants were detected in all 3 areas, but only P. vivax VK210 was found as a single agent of infection, while the other 2 occurred as mixed infections. The P. vivax-like variant was found to be associated with low parasitaemia and VK210 with the highest parasitaemia levels; none of the P. vivax variants was linked with a previous malaria experience. In all cases parasitaemia clearance was identical regarding the type of treatment and consequently it is not possible to confirm the previously reported correlation between P. vivax genotype and response to chloroquine.

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Simplified Diagnosis of Malaria Infection: GFM/PCR/Elisa a Simplified Nucleic Acid Amplification Technique by PCR/Elisa

Cited by 6 publications

References 5 publications

Padronização de teste multiparamétrico para a pesquisa de anticorpos IgG anti-T.cruzi, anti-T.pallidum, anti-P. vivax e anti-P falciparum, empregando a técnica de Dot-ELISA

Padronização de teste multiparamétrico para a pesquisa de anticorpos IgG anti-T.cruzi, anti-T.pallidum, anti-P. vivax e anti-P falciparum, empregando a técnica de Dot-ELISA

Identification of Staphylococcus aureus enterotoxins A and B genes by PCR-ELISA

Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment

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