Histopathology and immunocytochemical study of type 3 and type 4 complement receptors in the liver and spleen of dogs naturally and experimentally infected with Leishmania (Leishmania) chagasi

Tafuri, Wagner Luiz; Barbosa, Alfredo José Afonso; Michalick, Marilene Suzan Marques; Genaro, Odair; França-Silva, João Carlos; Mayrink, Wilson; Nascimento, Evaldo

doi:10.1590/s0036-46651996000200001

Cited by 52 publications

(49 citation statements)

References 23 publications

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“…11,12,28 Our finding of small liver granulomas, an indication of an active cell mediated immune response, in the intradermally infected dogs is consistent with what was observed in resistant dogs naturally infected in endemic areas. [29][30][31] However, the previous work by Killick-Kendrick and others 10 showed development of severe disease (timing or nature not specified) in 48% of dogs infected intradermally with approximately 10 5 L. infantum promastigotes isolated from the mid guts of infected sand flies. These dogs were followed for a prolonged period (5 years), whereas the dogs in our study were followed only for 1 year.…”

Section: Discussionmentioning

confidence: 98%

Clinical, Parasitologic, and Immunologic Evolution in Dogs Experimentally Infected with Sand Fly-Derived Leishmania chagasi Promastigotes

Travi¹,

Osorio²,

Saldarriaga³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. Experimental infection of dogs with Leishmania infantum has yielded heterogeneous clinical, parasitologic, and immunologic results. We studied dogs infected with 10 5 or 10 4 sand fly-derived promastigotes delivered by the intradermal (ID) or intravenous (IV) routes. Total mortality over 1 year post-infection reached 23.8%. The mortality and proportion of sustained polysymptomatic dogs was highest in the IV-10 5 group. The early appearance of polysymptoms was associated with an increased risk of progression to death. Dissemination of the parasite to lymph nodes was faster, and the subsequent infectivity to sand flies higher, in the IV compared with ID-infected dogs. Parasite-specific IgG1 or IgG2 production was similar among the groups, but higher interferon-γ (IFN-γ) and interleukin-10 (IL-10) expression was associated with polysymptomatic dogs. On the basis of the data obtained from this study, a sample size analysis using different endpoints for future vaccine trials is described.

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Section: Discussionmentioning

confidence: 98%

Clinical, Parasitologic, and Immunologic Evolution in Dogs Experimentally Infected with Sand Fly-Derived Leishmania chagasi Promastigotes

Travi¹,

Osorio²,

Saldarriaga³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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“…Numerous investigations have been directed towards determining the function of MP using alveolar and peritoneal MPs of human, mouse, rat and guinea-pig, and their MPs cell lines. Recently, some workers have been used cells from larger animals such as dogs and cats for further investigation because it is often difficult to obtain fresh cells regularly and in sufficient quantity from humans and smaller animals such as the mouse, rat and guinea-pig [10,18].…”

mentioning

confidence: 99%

Scanning Electron Microscopic and Cytochemical Analyses of Alveolar and Peritoneal Macrophages of Mice, Dogs and Cats.

Okamoto

Tanigawa

Tanaka

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. Alveolar and peritoneal macrophages (MPs) of mouse, dog and cat were compared in relation to their scanning electron microscopic features and the lysosomal activities of nonspecific esterase, acid phosphatase, and β-glucuronidase. The long spindle shape of peritoneal MPs differed from the spherical form of alveolar MPs in all species. There was no difference in the morphological findings among the three animals. Murine alveolar and peritoneal MPs were strongly positive for all three enzymes. Canine and feline alveolar and peritoneal MPs were strongly positive for acid phosphatase and β-glucuronidase, but weakly positive for nonspecific esterase. These results strongly suggest that acid phosphatase and β-glucuronidase can be used as markers of the MPs in healthy dogs and cats. -KEY WORDS: canine, cytochemistry, macrophage.J. Vet. Med. Sci. 60(10): 1145-1148, 1998 no abnormal findings in mice. Alveolar and peritoneal MPs were prepared using the methods of McCarron et al. [12] and Tanigawa et al. [19], respectively. In brief, alveolar fluids were obtained by repeated pulmonary lavage with cold phosphate-buffered saline (PBS, pH 7.2, 50-100 ml/ kg). Peritoneal fluids were obtained by washing the peritoneal cavity with cold PBS (50-100 ml/kg) at 3 days after intraperitoneal injection of 1.5% thioglycolate solution (50 mg/kg) in saline. Both fluids obtained were centrifuged, and sediments were washed. Their cellular concentration was countered with a hemocytometer. Light-Giemsa staining showed more than 90% of peritoneal exudate cells to be MPs. The cells were incubated with Dulbecco's modified Eagle's medium (Gibco, Grand Island, NY, U.S.A.), supplemented with 7.5% fetal calf serum (Microbiological Associates, Walkersville, MD, U.S.A.) in tissue culture dishes containing glass cover slips in an atmosphere of 10% CO 2 at 37°C for 2 hr. Scanning electron microscopy: Prepared cells were fixed with 3% glutaraldehyde in a 0.1 M phosphate (pH 7.3) solution for 2 hr and postfixed with 1% osmium tetroxide in the same buffer for 1 hr. After conductive-staining with 2% tannic acid and 1% osmium tetroxide, the cells were dehydrated in a graded series of ethanol baths, then immersed in t-butyl alcohol, and freeze-dried using the tbutyl alcohol freezing drying method [7]. An ion sputter coater was used to coat the dried samples with platinum, and the cells were observed under a field emission scanning electron microscope (HFS-2ST, Hitachi, Tokyo, Japan).Lysosomal enzymes: Prepared cells were fixed with cold 4% paraformaldehyde (pH 7.4) for 5 min and maintained in Holt's gum sucrose. The cells were then stained by the α-naphtyl acetate method [9] for the detection of nonspecific esterase, by the naphthol AS-BI phosphate method [2] for detection of acid phosphatase, and by the naphthol AS-BI β -glucuronidase method [6] for detection of β-glucuronidase. Examination under light microscopy showed

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“…Todavia, a hipertrofia e hiperplasia da polpa branca ocorreram em todos os grupos estudados, principalmente nos animais sintomáticos e assintomáticos. Essas alterações encontram-se descritas na literatura sobre animais naturalmente e experimentalmente infectados (6) . Os fragmentos de baço analisados apresentaram espessamento e inflamação da cápsula, com graus variados de intensidade.…”

Section: Resultsunclassified

“…A Leishmaniose Visceral Canina (LVC) caracteriza-se por apresentar um largo espectro de lesões que varia desde a infecção inaparente até uma forma clinica severa, que normalmente leva à morte do animal. As principais lesões da leishmaniose visceral ocorrem em órgãos ricos em células do sistema monocítico mononuclear como baço, fígado, linfonodos, medula óssea, rins, pulmões, intestinos e pele (5)(6)(7)(8) . O baço pode apresentar tamanho normal, discretamente aumentado ou hipertrofiado (9,10) .…”

Section: Introductionunclassified

ALTERAÇÕES DA MATRIZ EXTRACELULAR ESPLÊNICA EM CÃES NATURALMENTE INFECTADOS COM Leishmania (Leishmania) infantum chagasi

Martins

Coelho

Santos³

et al. 2015

Ciênc. anim. bras.

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Resumo:Objetivou-se estudar as alterações da matriz extracelular no baço de cães naturalmente infectados com Leishmania (Leishmania) infantum chagasi correlacionando-as com aspectos clínicos, histopatológicos e parasitológicos. Foram utilizados 18 cães, divididos em três grupos: seis animais não infectados (grupo controle) e doze animais infectados. Todos sem raça e idade definidas, provenientes da região do Município de São Luis-MA. Cortes parafinados do baço foram corados pela Hematoxilina e Eosina (H&E); Prata amoniacal de Gomori, para marcação das fibras reticulares e pela técnica Imuno-histoquímica da estreptoavidina-peroxidase para detecção de formas amastigotas de Leishmania. As análises morfométricas foram feitas utilizando-se o programa KS300 e o sistema de análise de imagens Kontron Elektronic/Carl Zeiss, Germany. Os resultados mostram que há um aumento significativo da deposição de fibras colágenas no baço quando comparadas aos animais controles, revelando diferenças significativas entre os animais sintomáticos e assintomáticos. Encontraram-se correlações positivas entre a presença do parasitismo tecidual e a deposição de colágeno. Os animais sintomáticos apresentaram uma maior deposição de colágeno no baço, que pode estar associada ao maior parasitismo tecidual encontrado. Os resultados demonstram que na leishmaniose visceral canina há uma fibrogênese intensa no baço, sendo esta associada ao parasitismo tecidual e a processos degenerativos decorrentes da doença.Palavras-chave: baço; Leishmania; matrix extracelular. Abstract:The aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with

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Histopathology and immunocytochemical study of type 3 and type 4 complement receptors in the liver and spleen of dogs naturally and experimentally infected with Leishmania (Leishmania) chagasi

Cited by 52 publications

References 23 publications

Clinical, Parasitologic, and Immunologic Evolution in Dogs Experimentally Infected with Sand Fly-Derived Leishmania chagasi Promastigotes

Clinical, Parasitologic, and Immunologic Evolution in Dogs Experimentally Infected with Sand Fly-Derived Leishmania chagasi Promastigotes

Scanning Electron Microscopic and Cytochemical Analyses of Alveolar and Peritoneal Macrophages of Mice, Dogs and Cats.

ALTERAÇÕES DA MATRIZ EXTRACELULAR ESPLÊNICA EM CÃES NATURALMENTE INFECTADOS COM Leishmania (Leishmania) infantum chagasi

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