Entamoeba histolytica: fecal antigen capture immunoassay for the diagnosis of enteric amebiasis by a monoclonal antibody

Urdaneta, Haideé; Rangel, Antonio; Martins, Marta; Muñoz, José F.; M, Manuel Hernández

doi:10.1590/s0036-46651996000100008

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…However, it reaches about 60% sensitivity and can give false-positives due to misidentification of non-pathogenic Entamoeba species [15][16][17][18]. The examination of fecal samples by optic microscope is not able to identify or differentiate E. histolytica from E. dispar unless erythrophagocytosis (the presence of ingested RBCs in trophozoites) is seen during microscopic examination [11,19].…”

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Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica

Santos¹,

Peralta²,

Macedo³

et al. 2007

Braz J Infect Dis

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Amebiasis is an infection caused by Entamoeba histolytica. However, differentiation between E. histolytica and Entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. The purpose of the present study was to evaluate a Multiplex -PCR for detection and differentiation of E. histolytica from E. dispar from fresh stool samples in comparison with the coproantigen commercial ELISA. Microscopic examination of stools using the Coprotest method, detection of stool antigen by enzyme-linked immunosorbent assay kit and a home made Multiplex-PCR, were used for the diagnosis of amoebiasis infection. Analysis of the 127 stools samples by microscopy examination demonstrated that only 27 (21%) samples were positive for E. histolytica/E. dispar complex. Among these stool samples, 11 were positive by Multiplex-PCR, with nine presenting the diagnostic fragment characteristic of E. dispar (96 bp) and two presenting diagnostic fragment of E. histolytica (132 bp). Among negative samples detected by microscopic examination, three positive samples for E. dispar and one positive for E. histolytica by Multiplex-PCR was observed. This denotes a low sensibility of microscopic examination when a single stool sample is analyzed. Assay for detection of E. histolytica antigen was concordant with multiplex-PCR in relation to E. histolytica. Statistical analysis comparing the sensibility tests was not done because of the low number of E. histolytica cases. The results demonstrate the importance of the specific techniques use for the differentiation between E. histolytica and E. dispar.

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confidence: 99%

Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica

Santos¹,

Peralta²,

Macedo³

et al. 2007

Braz J Infect Dis

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“…Singh et al (42), reported that the diagnosis of amoebiasis using coproantigen ELISA kits had an 89% sensitivity and 100% specificity. Urdaneta et al (43), showed that coproantigen ELISA is a more reliable method than the microscopic examination with 98.3% sensitivity and 97.6% specificity. The positive and negative predictive values were 96.2% and 97.6%, respectively, to detect E. histolytica in stool samples.…”

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confidence: 99%

Detection of Entamoeba histolytica Coproantigen Among Children with Dysentery in Ahvaz, Southwest Iran

Shamsizadeh

Nikfar

Rahdar

2020

Zahedan J Res Med Sci

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: Entamoeba histolytica is one of the important parasitic diseases in many parts of the world, especially the tropical and subtropical regions. The parasite is transmitted through contaminated water and vegetables. The exact diagnosis of infection with the parasite is crucial in many medical laboratories since there are many false positive and negative results in their reports. Therefore, the current study aimed at evaluating and comparing microscopic and coproantigen ELISA (the enzyme-linked immunosorbent assay) results to reach an appropriate test for the correct diagnosis of amoebiasis in children. One hundred stool samples were collected from children under 15 years old with dysenteric diarrhea from April to September 2018. Microscopic tests and coproantigen ELISA were performed on all the samples. The results showed that 5% of the samples had E. histolytica/E. dispar cysts. The findings of ELISA to detect coproantigen did not show any specific E. histolytica antigen in the samples. Hence, all the patients received chemotherapy for shigellosis. E. histolytica infection is not the main causative agent for dysenteric diarrhea in children in the studied area, and laboratory experts should be trained to prevent false-positive reports.

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“…Algunas pruebas serológicas han sido útiles en el diagnóstico indirecto de Ias formas extraintestinales de amebiasis, en particular dei absceso hepático (Madison, 1991). Sin embargo, para Ias formas intestinales estos exámenes no han representado una solución eficiente ai problema de su diagnóstico (Ravdini, 1995). Sistemas para Ia deteccíón de antígenos en heces han sido recientemente desarrollados (Haque et ai., 1993; Abd-Alla et ai.,1993; González-Ruíz et ai., 1994; Haque et ai., 1995; Urdaneta et ai., 1996; Jackson & Ravdin, 1996).…”

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Demostracion, Mediante Enzymeba, Del Sobrediagnóstico De Amebiasis Intestinal Asociado Al Examen Microscópico De Heces. Reporte De Un Estúdio en Cienfuegos, Cuba

Fonte¹,

Fernandez²,

Sánchez³

et al. 2007

Rev Patol Trop

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El sobrediagnóstico microscópico de amebiasis intestinal ha sido ampliamente reportado, pêro poças veces objetivãmente demostrado. En el presente trabajo, empleando Enzymeba, un procedimiento diagnóstico de amebiasis intestinal desarrollado en el Instituto de Medicina Tropical "Pedro Kourí", Ciudad de La Habana, abordamos este problema. Durante seis meses se colectaron cn los servidos de parasitologfa de Ia casi totalidad de los policlínicos (13 de 14), y de los dos mayorcs hospitales (2 de 3) de Ia província de Cienftiegos, todas Ias muestras de heces en Ias que el personal técnico correspondiente había informado Ia detección por observación microscópica de uno o más estádios de Entamoeba histolylica. En solo 61 (14.4%) de 424 muestras colectadas Enzymeba confirmo el hallazgo microscópico prévio. No encontramos diferencia estadísticatnente significativa (p>0.05) entre Ia eficiência dei diagnóstico microscópico en los hospitales y en los policlínicos de aquella província.PALABRAS CLAVES: Amebiasis. Sobrediagnóstico microscópico. Enzymeba. INTRODUCCIÓNEl examen microscópico de heces continua siendo el procedimiento de laboratório más ampliamente utilizado para el diagnóstico de amebiasis

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Entamoeba histolytica: fecal antigen capture immunoassay for the diagnosis of enteric amebiasis by a monoclonal antibody

Cited by 3 publications

References 25 publications

Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica

Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica

Detection of Entamoeba histolytica Coproantigen Among Children with Dysentery in Ahvaz, Southwest Iran

Demostracion, Mediante Enzymeba, Del Sobrediagnóstico De Amebiasis Intestinal Asociado Al Examen Microscópico De Heces. Reporte De Un Estúdio en Cienfuegos, Cuba

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