Species and serovars of enteropathogenic agents associated with acute diarrheal disease in Rosario, Argentina

Notario, Rodolfo; Borda, Noemí; Gambandé, Telma; Sutich, Emma

doi:10.1590/s0036-46651996000100002

Cited by 12 publications

(12 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The main E. coli pathotype found was Enteropathogenic Escherichia coli (EPEC, 04.2%). The picture is similar to a study in Argentina, where EPEC was isolated from nine cases (04.5%) of a total of 200 diarrhea patients [57]. EPEC is a well known enteropathogen.…”

Section: Discussionsupporting

confidence: 85%

Pathogenic Microorganisms Associated With Childhood Diarrhea in Low-and-Middle Income Countries: Case Study of Yaoundé – Cameroon

Yongsi

2008

IJERPH

View full text Add to dashboard Cite

Notwithstanding significant advancement in the understanding of pathogenesis and management, diarrheal illnesses remain one of the principal causes of global childhood mortality and morbidity. Infections account for most illnesses, with pathogens employing ingenious mechanisms to establish disease. In 2002, an interdisciplinary program “Populations et al. Espaces à Risques SANitaires” (PERSAN) was set up under the patronage of the Development Research Institute (IRD). Focused on health in Cameroon’s urban environment, the program mainly sought to identify diarrhea risk factors in Yaoundé. So for, a cross-sectional epidemiological study in children aged 6–59 months was carried out using a standardized protocol. The survey was initiated in 2002 and conducted during April to June in the year 2005. 3,034 stool samples were collected from children in twenty neighbourhoods in Yaoundé and examined at the Epidemiology and Public Health Laboratory of the Cameroon Pasteur Institute. About 60% of the patients were aged less than two years and 52% were male. Among the 437 patients with the diarrheal disease, 260 were found to be of infectious etiology, i.e. micro organism was detected in 59.5 % of the cases. Out of which, 10 (03.8%), 96 (36.9%), and 154 (59.2%) were respectively caused by pathogenic viruses, pathogenic bacteria and pathogenic parasites. Higher prevalence was found in overcrowded and under supply spontaneous settlement (78.4%) than in less crowded and formal residential settlement (21.5%). Etiologic data on diarrheal diseases and their spatial distribution are important tools for public health management and control strategic planning.

show abstract

Section: Discussionsupporting

confidence: 85%

Pathogenic Microorganisms Associated With Childhood Diarrhea in Low-and-Middle Income Countries: Case Study of Yaoundé – Cameroon

Yongsi

2008

IJERPH

View full text Add to dashboard Cite

show abstract

“…Salmonella was also commonly found in our patients, but no typhoidal specimens were isolated. In our sample, E. coli was the least frequent causative agent of acute diarrhea; however, it is more important to the epidemiology of diarrhea in poorer areas [10,11] [12], while it is much more common in other countries [13][14][15].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological and microbiological aspects of acute bacterial diarrhea in children from Salvador, Bahia, Brazil

Diniz-Santos¹,

Santana²,

Barretto³

et al. 2005

Braz J Infect Dis

View full text Add to dashboard Cite

In the few cases of acute childhood diarrhea that require antimicrobial therapy, the correct choice of the drug depends on detailed previous knowledge of local strains. In order to establish such parameters in our city, we reviewed the results of all 260 positive stool cultures of children between 0 and 15 years of age during two years at a pediatric tertiary care facility in Salvador, Brazil. Bacterial strains had been presumptively identified by culturing in selective media and by biochemical testing, and their antimicrobial susceptibility patterns were automatically detected by the MicroScan Walkaway System. Data about patients' sex and age, monthly distribution of the cases, pathogens isolated and their antimicrobial resistance patterns were recorded. Males corresponded to 55.4% of our sample, and most of our patients (42.7%) were between one and four years of age. Shigella was the commonest pathogen, being found in 141 (54.3%) cultures, while Salmonella was found in 100 (38.4%) cultures and Enteropathogenic E. coli in 19 (7.3%). Salmonella was the main causal agent of diarrhea in children younger than five years old, whereas Shigella was the most frequent pathogen isolated from the stools of children between five and 15 years old. The peaks of incidence correspond to the periods of school vacations. Shigella specimens presented a very high resistance rate to trimethoprim-sulfamethoxazole (90.1%) and to ampicillin (22.0%), while Salmonella presented very low resistance rates to all drugs tested. These data are useful for practitioners and they reinforce the need for continuous microbiological surveillance.

show abstract

“…Although the majority of Campylobacter infections are caused by C. jejuni, other Campylobacter species, e.g., C. coli, C. lari, and C. upsaliensis, have been associated with either sporadic (10,28,37,39,52,55,65) or outbreak (8,26,40,57) cases of gastroenteritis in humans. C. jejuni and C. coli are often isolated from the same hosts (48).…”

mentioning

confidence: 99%

Extended Multilocus Sequence Typing System for Campylobacter coli , C. lari , C. upsaliensis , and C. helveticus

On²,

et al. 2005

View full text Add to dashboard Cite

A multilocus sequence typing (MLST) system has been reported previously for Campylobacter jejuni to both differentiate strains and identify clonal lineages. However, sequence variation at the MLST loci prevents its use for closely related Campylobacter species. We describe herein an expanded MLST method to include three clinically relevant Campylobacter species, C. coli, C. lari, and C. upsaliensis, and a fourth Campylobacter species, C. helveticus. The C. coli and C. helveticus methods use the same seven C. jejuni loci (aspA, atpA, glnA, gltA, glyA, pgm, and tkt); however, adk and pgi were substituted for aspA and gltA in C. lari and for gltA and pgm in C. upsaliensis. Multiple C. coli (n ‫؍‬ 57), C. lari (n ‫؍‬ 20), C. upsaliensis (n ‫؍‬ 78), and C. helveticus (n ‫؍‬ 9) isolates, representing both clinical and environmental sources, were typed. All four species were genetically diverse: the majority (>80%) of the isolates had unique sequence types (STs). Using this method, mixed C. lari, C. upsaliensis, and C. helveticus isolates were identified; upon separation, each isolate was shown to contain two strains of the same species with distinct STs. Additionally, the expanded MLST method was able to detect potential lateral transfer events between C. jejuni and either C. coli or C. lari and between C. upsaliensis and C. helveticus. Thus, the expanded MLST method will prove useful in differentiating strains of five Campylobacter species, identifying mixed Campylobacter cultures, and detecting genetic exchange within the genus.

show abstract

Species and serovars of enteropathogenic agents associated with acute diarrheal disease in Rosario, Argentina

Cited by 12 publications

References 6 publications

Pathogenic Microorganisms Associated With Childhood Diarrhea in Low-and-Middle Income Countries: Case Study of Yaoundé – Cameroon

Pathogenic Microorganisms Associated With Childhood Diarrhea in Low-and-Middle Income Countries: Case Study of Yaoundé – Cameroon

Epidemiological and microbiological aspects of acute bacterial diarrhea in children from Salvador, Bahia, Brazil

Extended Multilocus Sequence Typing System for Campylobacter coli , C. lari , C. upsaliensis , and C. helveticus

Contact Info

Product

Resources

About