Parasitological diagnosis of schistosomiasis mansoni: concomitant utilization of Kato-Katz method and hatching test

Lemos, Clarice P. Abrantes; Lima, Danielle Malta; Silva, Luiz Caetano da; Chieffi, Pedro Paulo

doi:10.1590/s0036-46651995000500015

Cited by 5 publications

(1 citation statement)

References 3 publications

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“…Para Lemos e cols, o emprego de técnicas simultâneas leva à obtenção de melhores esultados 21 . Mendes e cols afirmam que, como rotina em laboratório, utiliza-se mais de um método coproscópico para a detecção de formas parasitárias de protozoários e helmintos, principalmente em amostras com baixa carga parasitária.…”

Section: Resultados Discussãounclassified

Prevalência da infecção pelo Schistosoma mansoni em dois municípios do Estado de Alagoas

Palmeira

Carvalho

Rodrigues

et al. 2010

Rev. Soc. Bras. Med. Trop.

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RESUMO ABSTRACTIntroduction: Preliminary studies indicate a probable schistosomiasis expansion in Alagoas. This work analyzes the occurrence of Schistosoma mansoni in students from two municipalities, located in the Mundaú and Paraíba rivers basins. Methods: The study group consisted of 690 students in the urban area, aged between seven and 15 years. A standardized socio-economic questionnaire was filled out and parasitological tests were used to diagnosis schistosomiasis (Lutz and Kato methods). Positive cases were treated and students and their families were lectured about health education. Results: The Schistosoma mansoni prevalence was 24.9%. The association between schistosomiasis and other parasitic diseases was significant in relation to trichuriasis (p <0.05). Only 50% of infected children lived in brick homes with sanitation and public water supply. Among them, 48.1% used to go usually to the water collections and 55.8% of them knew rivers snails. The concordance analysis between the Lutz and Kato methods showed disagreement in 54.2%, with greater sensitivity for the Lutz technique (76.4%). Conclusions: The indices obtained characterize the municipalities as hyperendemic areas, because prevalence rates over 5% are considered high according to the World Health Organization. The high prevalence observed here is also two times higher than that reported by the Brazilian Schistosomiasis Program Control.

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Section: Resultados Discussãounclassified

Prevalência da infecção pelo Schistosoma mansoni em dois municípios do Estado de Alagoas

Palmeira

Carvalho

Rodrigues

et al. 2010

Rev. Soc. Bras. Med. Trop.

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Seeding experiments demonstrate poor performance of the hatching test for detecting small numbers of Schistosoma mansoni eggs in feces

Borges

Souza

Romanzini

et al. 2013

Parasitology International

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An IL-13 Promoter Polymorphism Associated with Liver Fibrosis in Patients with Schistosoma japonicum

et al. 2015

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The aim of this study was to determine whether two polymorphisms in the gene encoding IL13 previously associated with Schistosoma hematobium (S. hematobium) and S. mansoni infection are associated with S. japonicum infection. Single nucleotide polymorphisms (SNPs) rs1800925 (IL13/-1112C>T) and rs20541 (IL13R130Q) were genotyped in 947 unrelated individuals (307 chronically infected, 339 late-stage with liver fibrosis, 301 uninfected controls) from a schistosomiasis-endemic area of Hubei province in China. Regression models were used to evaluate allelic and haplotypic associations with chronic and late-stage schistosomiasis adjusted for non-genetic covariates. Expression of IL-13 was measured in S. japonicun-infected liver fibrosis tissue and normal liver tissue from uninfected controls by immunohistochemistry (IHC). The role of rs1800925 in IL-13 transcription was further determined by Luciferase report assay using the recombinant PGL4.17-rs180092 plasmid. We found SNP rs1800925T was associated with late-stage schistosomiasis caused by S. japonicum but not chronic schistosomiasis (OR = 1.39, 95%CI = 1.02–1.91, p = 0.03) and uninfected controls (OR = 1.49, 95%CI = 1.03–2.13, p = 0.03). Moreover, the haplotype rs1800925T-rs20541C increased the risk of disease progression to late-stage schistosomiasis (OR = 1.46, p = 0.035), whereas haplotype rs1800925C-rs20541A showed a protective role against development of late-stage schistosomiasis (F = 0.188, OR = 0.61, p = 0.002). Furthermore, S. japonicum-induced fibrotic liver tissue had higher IL13 expression than normal liver tissue. Plasmid PGL4.17-rs1800925T showed a stronger relative luciferase activity than Plasmid PGL4.17-rs1800925C in 293FT, QSG-7701 and HL-7702 cell lines. In conclusion, the functional IL13 polymorphism, rs1800925T, previously associated with risk of schistosomiasis, also contributes to risk of late-stage schistosomiasis caused by S. japonicum.

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Parasitological diagnosis of schistosomiasis mansoni: concomitant utilization of Kato-Katz method and hatching test

Cited by 5 publications

References 3 publications

Prevalência da infecção pelo Schistosoma mansoni em dois municípios do Estado de Alagoas

Prevalência da infecção pelo Schistosoma mansoni em dois municípios do Estado de Alagoas

Seeding experiments demonstrate poor performance of the hatching test for detecting small numbers of Schistosoma mansoni eggs in feces

An IL-13 Promoter Polymorphism Associated with Liver Fibrosis in Patients with Schistosoma japonicum

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