Gelatin particle indirect agglutination test for serodiagnosis of schistosomiasis: comparative study with enzyme-linked immunosorbent assay

Kobayashi, Jun; Carrilho, Flair José; Shimabukuro, Tetsu; Silva, Luiz Caetano da; Soares, Elza Cotrim; Nishimura, Nancy F.; Chieffi, Pedro Paulo; Sato, Yoshiya

doi:10.1590/s0036-46651994000500001

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Particles of gelatin were artificial spherical material made from gelatin and Arabic gum. It was used as an antigen carrier in this study [28,66]. Gelatin particles were washed three times by cold PBS (PH 6.4) and centrifuged (1200 RBM/10 min) 4-5 times.…”

Section: Gpiatmentioning

confidence: 99%

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Strongyloides stercoralis in Patients on Corticosteroids Therapy Using Enzyme-Linked Immunosorbent Assay and Gelatin Particles Indirect Agglutination Tests: A Diagnostic Approach

et al. 2019

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Introduction Patients on corticosteroids therapy (POCT) are more likely to flare up concealed Strongyloides infection and develop Strongyloides hyperinfection syndrome and/or dissemination. Such critical complications can lead to high mortality rates. Rapid detection methods are, therefore, necessary to detect Strongyloides infection in POCT with the advantage of being applicable in a developing country. Methods Two hundred POCT have been enrolled in this study to determine the rate of infection with Strongyloides. Three different groups of POCT (Strongyloides infected, non-infected, infected with other parasites) were used to evaluate the antibodies detection capability of two serological techniques (enzyme-linked immunosorbent assay (ELISA) and gelatin particles indirect agglutination (GPIAT) against the results of the gold standard agar plate culture (APC). Results With APC, the infection rate of Strongyloides stercoralis in POCT was 9.5% (19/200). POCT with Strongyloides infection displayed related risk factors (job, rural settlements, and soil contact) for infection combined with the subtropical nature of Ismailia Governorate. With regard to serology, ELISA detection results were poor compared to APC with sensitivity and specificity of 42.1% and 82.6%, respectively, and positive and negative predictive values of 72% and 30%. GPIAT appeared to be closely related to APC with sensitivity and specificity of 89.4% and 81.8%, respectively, and positive and negative predictive values of 80.9% and 96.7%. Statistical moderate correlation was detected between GPIAT and ELISA. ConclusionThe GPIAT technique is more convenient, easier, cheaper and faster to rule out the infection of Strongyloides in POCT. It might be the test of choice for routine immunodiagnosis of human strongyloidiasis.

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Section: Gpiatmentioning

confidence: 99%

“…The gelatin particle indirect agglutination test (GPIAT) is another interesting serum-based immunological assay that Sato et al first tested on Strongyloides [55]. GPIAT was reported to be highly sensitive compared to ELISA for the diagnosis of Strongyloides [22,39,53,60] and other parasites [28,72].…”

Section: Introductionmentioning

confidence: 99%

Strongyloides stercoralis in Patients on Corticosteroids Therapy Using Enzyme-Linked Immunosorbent Assay and Gelatin Particles Indirect Agglutination Tests: A Diagnostic Approach

et al. 2019

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Diagnostic tests for Schistosomiasis for low prevalence settings: a systematic review and Meta-Analysis

Vaillant

Philippy

Barré

et al. 2021

Preprint

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Background Tests for diagnosing schistosomiasis in areas where prevalence is low due to control programme of the disease should be suffiently sensitive to detect the residual disease. If they had sufficient diagnostic accuracy they could replace conventional microscopy as they provide a quicker answer and are easier to use. Objectives To compare sensitivity and specificity of new tests, especially rapid diagnostic tests (RDTs), with regard to a certain reference test. Search methods We searched the electronic databases Pubmed, EMBASE, the Cochrane Library and LILACS up to February 2021. Furthermore we searched results from the previous meta-analyses. Selection criteria We included studies that used microscopy as the reference standard: for S. haematobium, microscopy of urine prepared by filtration, centrifugation, or sedimentation methods; and for S. mansoni, microscopy of stool by Kato-Katz thick smear. Data collection and analysis Two review authors independently extracted data, assessed quality of the data using QUADAS-2, and performed meta-analysis where appropriate. Grading of evidence was done with the GRADE methodology by using GradePro. Using the variability of test thresholds, we used a bayesian bivariate random-effects summary receiver operating characteristic model for all eligible tests. We investigated heterogeneity, and carried out sensitivity analyses where possible. Results for sensitivity and specificity are presented as percentages with 95% confidence intervals (CI). Main results The review gathered 203 articles stating a diagnostic test for the diagnosis of S. haematobium and S. mansoni out of which 114 entered the analyses. Microscopy of Urine filtration or Kato-Katz smears were used as the reference standard. Compared with Kato-Katz smears, AWE-SEA ELISA (Se=94%; Sp=64%) is comparable to CCA1 (Se=87%; Sp=60%). IgG ELISA (Se=93%; Sp=68%) has also a very good ability to detect true positive as well as CAA cassette (Se=73%; Sp=68%). For S. haematobium, proteinuria (Se=59%; Sp=83%) and haematuria (Se=74%; Sp=87%) reagent strips showed reasonably high specificities with a considerably better sensitivity for the haematuria test. Authors' conclusions There are interesting promising new diagnostic tests that were tested in field studies. However prevalences of the locations where these studies took place are variable and there are no specific study with a high number of patients in areas with low level of schistosomiasis infection.

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Correction to: Strongyloides stercoralis in Patients on Corticosteroids Therapy Using Enzyme‑Linked Immunosorbent Assay and Gelatin Particles Indirect Agglutination Tests: A Diagnostic Approach

et al. 2019

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Gelatin particle indirect agglutination test for serodiagnosis of schistosomiasis: comparative study with enzyme-linked immunosorbent assay

Cited by 3 publications

References 18 publications

Strongyloides stercoralis in Patients on Corticosteroids Therapy Using Enzyme-Linked Immunosorbent Assay and Gelatin Particles Indirect Agglutination Tests: A Diagnostic Approach

Strongyloides stercoralis in Patients on Corticosteroids Therapy Using Enzyme-Linked Immunosorbent Assay and Gelatin Particles Indirect Agglutination Tests: A Diagnostic Approach

Diagnostic tests for Schistosomiasis for low prevalence settings: a systematic review and Meta-Analysis

Correction to: Strongyloides stercoralis in Patients on Corticosteroids Therapy Using Enzyme‑Linked Immunosorbent Assay and Gelatin Particles Indirect Agglutination Tests: A Diagnostic Approach

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