Measles serodiagnosis: standardization and evaluation of a dot-ELISA

Lima, L R A Vaz de; Hoshino‐Shimizu, Sumie; Souza, Vanda Akico Ueda Fick de; Pannuti, Cláudio Sérgio; Andrade, Heitor Franco de; Sumita, Laura Masami; Ferreira, Antônio Walter

doi:10.1590/s0036-46651994000200008

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…HSV, 27 measles virus, 28 EBV 29 and HCV. 30 IgM missing in a certain percentage of cases during acute infections has been reported for parvovirus B 19, 31 HAV, 32 EBV, 29 Poliomyelitis virus, 33 measles virus, 34 Japanese encephalitis virus, 35 tick-borne encephalitis virus, 36 rubella virus, 37 HCV, 30 and CMV. 38 Whereas missing or apparently late appearing IgM responses may have caused misdiagnosis of acute infections as past infections, the opposite problem was arising through the detection of persistently positive IgM responses for time periods of many months or even few years, as found for borrelia infection, 39 rubella virus, 40,41 EBV, 29 Puumala virus, 42 parvovirus B 19, 43 HCV, 30 and TBE virus.…”

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confidence: 99%

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The variability of the serological response to SARS‐corona virus‐2: Potential resolution of ambiguity through determination of avidity (functional affinity)

Bauer

2020

Journal of Medical Virology

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Data on the serological response towards SARS CoV‐2 in 16 recent reports were analyzed and a high degree of variability was shown. IgM responses were either found earlier than IgG, or together with IgG, later than IgG or were missing. Therefore, clear distinctions between early, intermediate and past infections are obviously not possible merely on the basis of IgM and IgG determinations. The review of publications on the serology of other virus groups shows that variable IgM responses can be found as well and therefore are not specific for SARS CoV‐2 infections. A model to explain this variability is proposed. The inclusion of avidity determination into regular diagnostic procedures has allowed to resolve such “atypical” serological constellations. The potential use of avidity determination for the diagnosis of Covid‐19, for risk assessment, epidemiological studies, analysis of cross reactions, as well as for the control of vaccination programs is suggested and discussed. This article is protected by copyright. All rights reserved.

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confidence: 99%

“…IgM missing in a certain percentage of cases during acute infections has been reported for parvovirus B 19, 31 HAV, 32 EBV, 29 Poliomyelitis virus, 33 measles virus, 34 Japanese encephalitis virus, 35 tick‐borne encephalitis virus, 36 rubella virus, 37 HCV, 30 and CMV 38 …”

Section: Introductionmentioning

confidence: 99%

The variability of the serological response to SARS‐corona virus‐2: Potential resolution of ambiguity through determination of avidity (functional affinity)

Bauer

2020

Journal of Medical Virology

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“…The so obtained MV permitted us to prepare different types of antigen, and in particular a deoxycholate-based antigen showed to be suitable for enzyme immunoassays such as IgG ELISA and IgG dot-ELISA, as well as IgM dot-ELISA (7).…”

Section: Introductionmentioning

confidence: 99%

Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent

Hoshino‐Shimizu

Vaz-de-Lima

Oliveira

et al. 2001

Braz. J. Microbiol.

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Recent measles outbreaks in different countries led to an increase of laboratory measles diagnosis. Thus, we developed the IgM-Measles ELISA IAL , using measles virus antigens obtained from cell cultured in microcarriers in order to supply reagent kits to Brazilian public health laboratories. A batch of antigenic reagent was produced and evaluated in the enzyme immunoassay in comparison with clinical diagnosis and with as reference assay (IgM Capture ELISA CDC ) data. This study was performed in a positive panel with 70 serum samples from patients with measles, and a negative panel with 132 samples from patients with unrelated diseases and without recent measles or vaccination history. In relation to other diagnostic methods, the IgM ELISA IAL presented sensitivity higher than 97.1%, specificity and precision of 97%, and agreement kappa (k) index higher than 0.94 (P < 0.05). Moreover, the IgM antibody profile from measles acute phase revealed by the assay was similar to the reference assay. A practical analysis system for checking the quality of new reagent batches was proposed based on the diagnostic features and agreement kappa index. Our findings suggest that measles antigenic reagents can be produced with reliable quality control system, and supplied to public health laboratories for routine serodiagnosis or population surveys.

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“…Current IgG and IgM assays are based on whole MV or virus-infected cells as antigens (8,10,11,33,35,36,40). These antigens are possibly more costly and difficult to produce and preserve under standardized conditions than are recombinant proteins.…”

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confidence: 99%

Evaluation of Hemagglutinin Protein-Specific Immunoglobulin M for Diagnosis of Measles by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Protein Produced in a High-Efficiency Mammalian Expression System

et al. 1998

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Recombinant hemagglutinin (H) of the measles virus (MV) expressed in a mammalian high-expression system based on the Semliki Forest virus replicon was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG in patients with acute-phase measles. One hundred twelve serum specimens from 70 patients with measles were analyzed. Case definition was based on a commercial IgM ELISA that utilizes MV-infected cells (MV-ELISA) (Enzygnost; Behring Diagnostics); the clinical criteria of the Centers for Disease Control and Prevention (Atlanta, Ga.); and/or the increase in hemagglutinin test titers, neutralization test titers, and levels of MV-specific IgG whenever paired sera were available. The initial time courses of the IgM signal after the onset of rash are similar in the H- and MV-ELISAs. On days 0 to 19, both ELISAs detected IgM in 67 of 68 (98.5%) sera. Average maximal levels of IgM seem to persist, however, about 10 days longer in the MV-ELISA (up to day 25) than in the H-ELISA (day 15). From days 20 to 29 and 30 to 59, the H-ELISA detected only 64.3 (9 of 14) and 19.2% (5 of 26), respectively, of sera that were IgM positive by MV-ELISA. At least up to day 30, the performance of the H-ELISA seemed to be similar to that reported for commercial ELISAs based on whole MV. Our results demonstrate that MV H-specific IgM can be used to diagnose most measles cases from a single serum specimen collected within 19 days after the onset of rash and that the recombinant protein used in this study is suitable for this purpose.

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Measles serodiagnosis: standardization and evaluation of a dot-ELISA

Cited by 5 publications

References 16 publications

The variability of the serological response to SARS‐corona virus‐2: Potential resolution of ambiguity through determination of avidity (functional affinity)

The variability of the serological response to SARS‐corona virus‐2: Potential resolution of ambiguity through determination of avidity (functional affinity)

Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent

Evaluation of Hemagglutinin Protein-Specific Immunoglobulin M for Diagnosis of Measles by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Protein Produced in a High-Efficiency Mammalian Expression System

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