Desenvolvimento do Schistosoma mansoni na cavidade peritoneal de camundongos da linhagem AKR/J

Abstract

Cercárias de Schistosoma mansoni, inoculadas na cavidade peritoneal de camundongos da linhagem AKR/J, conseguiram sobreviver in situ e chegar à maturidade sexual. Ao contrário da linhagem convencional (SWISS), onde as fêmeas que se desenvolveram no peritônio não produziram ovos, 7,7% das fêmeas retiradas da cavidade peritoneal de camundongos AKR/J apresentavam ovo normal no útero. Os parasitos recuperados da cavidade peritoneal de ambas as linhagens não apresentaram pigmento hemoglobínico, indicando que os mesmos sobrevivem na cavidade peritoneal de camundongos sem a necessidade de ingestão de hemácias. O desenvolvimento do parasito na cavidade peritoneal de camundongos AKR/J, com produção de ovos normais, reforça os dados, já existentes na literatura, que mostram que o ciclo evolutivo do parasito pode ser completado sem a necessidade da fase pulmonar.

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

“…In fact, up to now, this strain of rodents had not been utilized either in the evaluation of immunomodulatory drugs in the course of this parasitic infection, or in oogram analysis. AKR/J mice present deficiency in the complement system, a high incidence of leukaemia (Ben-Efraim & Cinader, 1964; Wheat et al , 1987), and in the peritoneal cavity of these rodents the complete development of the trematode occurs, reaching sexual maturity and laying eggs in situ (Bicalho et al , 1993; Bicalho, 2003; Mati, 2009). Therefore, AKR/J mice characterize a permissive and interesting model for research on S. mansoni biology.…”

“…Worms were then recovered from the portal system through perfusion (Pellegrino & Siqueira, 1956). Also, in order to obtain parasitic forms from the peritoneal cavity, a methodology previously described was employed (Pereira et al , 1974; Bicalho et al , 1993; Bicalho, 2003). All worms and free granulomas were inspected, counted and fixed in 10% formalin.…”

Phenotypic plasticity of maleSchistosoma mansonifrom the peritoneal cavity and hepatic portal system of laboratory mice and hamsters

“…Although it is not the usual site of development in natural infections, the intraperitoneal development of S. mansoni has been reported. When cercariae of S. mansoni are inoculated into the peritoneal cavity of rodents, different results are obtained in relation to the worm burden that remains intraperitoneally, as well as the total body length of the worms and their maturation (Moore & Meleney, 1955; Pereira et al , 1972; Bicalho et al , 1993; Fan & Pao, 2006). As an advantage, peritoneal infection allows a simple and rapid method for mass screening of prophylactic agents to combat schistosomiasis (Pereira et al , 1974).…”

EXPERIMENTAL INFECTION OF SWISS AND AKR/J MICE WITH Centrocestus formosanus (TREMATODA: HETEROPHYIDAE)

“…However, studies on trematodes using the AKR strain remain scarce and address experimental infection using the species Brachylaima cribbi 6 , Schistosoma mansoni 4,15,27 and Zygocotyle lunata 2 . The permissiveness of AKR/J mice towards some of these helminths has been correlated to their immunological peculiarities such as deficiency in the complement pathway 3,4 , polarization of the T helper cell (Th)-1 response 12 , and a less substantial increase in the number of goblet cells associated with mucin deficiency in mucosa during experimental intestinal infection 17 .…”

“…It is possible that the immunological characteristics of the AKR/J strain are related to this finding. In fact, the fecundity of helminths seems to be directly linked to the host immune system and may be influenced by immunological variations correlated with strains of mice 4,22 , immunomodulatory drugs 20,26 and experimental co-infection 5,15 .…”