Evaluación del método de ELISA para detección de antígenos y complejos inmunes circulantes de Trypanosoma cruzi a través de un estudio de campo en una zona endémica de Argentina

Petray, Patricia; Bonardello, Nelsa; Clark, Roberto; Agranatti, Mercedes; Corral, Ricardo S.; Grinstein, S

doi:10.1590/s0036-46651992000200010

Cited by 7 publications

(2 citation statements)

References 11 publications

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“…It is possible that EVs released from infected host cells may in part account for T. cruzi antigens previously detected in the circulatory system or urine of infected patients (72)(73)(74)(75), making EVs an attractive and tractable biological tool for facilitating direct antigen detection in fluids of Chagas disease patients. Indeed, EVs are emerging as a unique mechanism for enriching low-level antigens for cancer diagnosis (76), and recent studies using T. cruzi-specific RNA aptamers detected antigens in TESA preparations or serum from mice with acute or chronic T. cruzi infection (77).…”

Section: Discussionmentioning

confidence: 99%

Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

et al. 2017

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Chagas disease, caused by Trypanosoma cruzi, although endemic in many parts of Central and South America, is emerging as a global health threat through the potential contamination of blood supplies. Consequently, in the absence of a gold standard assay for the diagnosis of Chagas disease, additional antigens or strategies are needed. A proteomic analysis of the trypomastigote excreted-secreted antigens (TESA) associated with exosomal vesicles shed by T. cruzi identified ϳ80 parasite proteins, with the majority being trans-sialidases. Mass spectrometry analysis of immunoprecipitation products performed using Chagas immune sera showed a marked enrichment in a subset of TESA proteins. Of particular relevance for diagnostic applications were the retrotransposon hot spot (RHS) proteins, which are absent in Leishmania spp., parasites that often confound diagnosis of Chagas disease. Interestingly, serological screens using recombinant RHS showed a robust immunoreactivity with sera from patients with clinical stages of Chagas ranging from asymptomatic to advance cardiomyopathy and this immunoreactivity was comparable to that of crude TESA. More importantly, no cross-reactivity with RHS was detected with sera from patients with malaria, leishmaniasis, toxoplasmosis, or African sleeping sickness, making this protein an attractive reagent for diagnosis of Chagas disease.

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Section: Discussionmentioning

confidence: 99%

Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

et al. 2017

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“…The major disadvantage of serological tests is cross-reactions ( Jiang et al., 2021 ). In order to reduce the possibility of cross-reaction, antigenic fractions of the parasite in the host circulation should be used in serological methods ( Petray et al., 1992 ; Chen et al., 2008 ). No cross-reactivity against polyclonal anti-rBoSA1 antibodies in the sera of different animal species which were positive for the apicomplexan parasites, including B. bovis , B. bigemina , B. caballi, B. canis, B. gibsoni, T. equi , and T. annulata was detected in this study.…”

Section: Discussionmentioning

confidence: 99%

Babesia ovis secreted antigen-1 is a diagnostic marker during the active Babesia ovis infections in sheep

Sevinc,

Zhou,

Cao

et al. 2023

Front. Cell. Infect. Microbiol.

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Ovine babesiosis caused by Babesia ovis is an economically significant disease. Recently, a few B. ovis-specific proteins, including recombinant B. ovis secreted antigen-1 (rBoSA1), have been identified. Immunological analyses revealed that rBoSA1 resides within the cytoplasm of infected erythrocytes and exhibits robust antigenic properties for detecting anti-B. ovis antibodies. This protein is released into the bloodstream during the parasite’s development. It would be possible to diagnose active infections by detecting this secretory protein. For this purpose, a rBoSA1-specific polyclonal antibody-based sandwich ELISA was optimized in this study. Blood samples taken from the naturally (n: 100) and experimentally (n: 15) infected sheep were analyzed for the presence of native BoSA1. The results showed that native BoSA1 was detectable in 98% of naturally infected animals. There was a positive correlation between parasitemia level in microscopy and protein density in sandwich ELISA. Experimentally infected animals showed positive reactions from the first or second day of inoculations. However, experimental infections carried out by Rhipicephalus bursa ticks revealed the native BoSA1 was detectable from the 7th day of tick attachment when the parasite began to be seen microscopically. Sandwich ELISA was sensitive enough to detect rBoSA1 protein at a 1.52 ng/ml concentration. Additionally, no serological cross-reactivity was observed between animals infected with various piroplasm species, including Babesia bovis, B. bigemina, B. caballi, B. canis, B. gibsoni, Theileria equi, and T. annulata. Taken collectively, the findings show that the rBoSA1-specific polyclonal antibody-based sandwich ELISA can be successfully used to diagnose clinical B. ovis infections in sheep at the early stage.

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A novel Trypanosoma cruzi secreted antigen as a potential biomarker of Chagas disease

Nagarkatti

Acosta

Acharyya

et al. 2020

Sci Rep

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Chagas drug discovery has been hampered by a lack of validated assays to establish treatment efficacy in pre-clinical animal models and in patients infected with T. cruzi. Reduced levels of parasite secreted antigens in the blood of infected hosts could be used to demonstrate treatment efficacy. A published proteomic study of parasite secreted antigens identified the hypothetical protein Tc_5171 as a secreted antigen. In this report, we developed Tc_5171 specific antibodies and showed that the native protein was expressed by the three life cycle stages of the parasite. Anti-peptide antibodies were able to detect the parasite antigen in blood of infected mice during the acute and the chronic phase of infection. Benznidazole treatment of infected mice significantly reduced their blood antigen levels. Of clinical significance, patients diagnosed with Chagas disease, either asymptomatic or with cardiac clinical symptoms had significantly higher Tc_5171 antigen levels compared to endemic controls. Pair-wise analysis, before and after Benznidazole treatment, of patients with asymptomatic Chagas disease showed a significant reduction in antigen levels post treatment. Taken together, our results indicate that Tc_5171 could be used as a novel biomarker of Chagas disease for diagnosis and to assess treatment efficacy.

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Evaluación del método de ELISA para detección de antígenos y complejos inmunes circulantes de Trypanosoma cruzi a través de un estudio de campo en una zona endémica de Argentina

Cited by 7 publications

References 11 publications

Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

Babesia ovis secreted antigen-1 is a diagnostic marker during the active Babesia ovis infections in sheep

A novel Trypanosoma cruzi secreted antigen as a potential biomarker of Chagas disease

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