Plasmodium vinckei petteri: algunos aspectos de su esporogonia y esquizogonia exoeritrocitaria

Abstract

Por medio de la infección experimental de Anopheles stephensi, a partir de ratones portadores de gametocitos de Plasmodium vinckei petteri, se realizó el estudio morfológico de la esporogonia, que comprende: la descripción del ooquineto, la evolución completa de los ooquistes y su transformación final en esporozoitos. Estos fueron empleados más tarde para infectar por via intravenosa nuevos ratones, a los que se realizó biopsias sucesivas, para el estudio de los esquizontes exoeritrocíticos, cuya morfología no difere de la de otras especies del grupo. Se determinó la duración mínima del ciclo hepático, que resultó ser de 61 horas. Estos datos, junto a otros que refieren las características del ciclo hemático, ayudan a completar la información que se tiene sobre la especie, que la hacen recomendable para su utilización como modelo experimental en el estudio de la malaria humana.

Blood Feeding and Plasmodium Infection Alters the miRNome of Anopheles stephensi

“…We noticed distinct variation in expression pattern of miRNAs in the following corresponding conditions, namely 42 hrs and 5 days post Plasmodium infection. In order to understand Plasmodium stage-specific regulation, the miRNAs were profiled at two specific conditions coinciding with effect of parasite first invasion in Anopheles midgut at 42 hrs and late phase of Plasmodium development in midgut [37] . It is interesting to note that distinct subsets of miRNAs showed regulation in the sets analysed namely iBF 42 h vs BF 42 h and iBF 5d vs iBF 42 h. In iBF 42 h, four miRNAs (miR-124, miR-137, miR-1000, miR-932) were significantly up-regulated compared to BF 42 h. However, none of the identified miRNAs were down-regulated in iBF 42 h compared to BF 42 h. In iBF 5d, four miRNAs (miR-1175-3p, miR-1174, miR-281-3p and miR-281-5p) were significantly up-regulated, whereas 10 miRNAs (miR-285, miR-2944a-5p, miR-309, miR-210-3p, miR-1891, miR-981, miR-315-5p, miR-932, miR-124 and miR-7) were significantly down-regulated when compared with iBF 42 h showing parasite stage specific expression in mosquito ( Table 2 , Figure 5 ).…”

Inference of the Oxidative Stress Network in Anopheles stephensi upon Plasmodium Infection

“…Analysis of such a cellular state is likely to reveal metabolic homeostasis facilitating parasite maturation and most importantly minimal metabolic penalty on vector. We dissected the midguts at day 5 post infection and processed those midguts that had maximum oocysts [40] . Total RNA was isolated from the midgut of mosquitoes at different time points and at different conditions over several feeding experiments (minimum of three times).…”