Método de Faust et al.: rendimento de colheita por alça metálica

Pereira, Dante D.; Ferreira, Cláudio Santos

doi:10.1590/s0036-46651991000200011

Cited by 2 publications

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“…Subsequently the sediment was re-suspended with 7 mL of zinc sulfate solution (sp gravity 1.200). The flotation was performed with centrifugation for 2 min at 640 g. Five loops of the convex meniscus were collected with a bacteriological loop, as recommended by Pereira and Ferreira 26 . They were placed on a microscope slide, stained with a drop of Lugol’s iodine and covered with a coverslip.…”

Section: Methodsmentioning

confidence: 99%

The influence of serial fecal sampling on the diagnosis of giardiasis in humans, dogs, and cats

Uchôa

Sudré

Macieira

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Giardia infection is a common clinical problem in humans and pets. The diagnosis of giardiasis is challenging as hosts intermittently excrete protozoan cysts in their feces. In the present study, we comparatively evaluated two methods of serial fecal sampling in humans, dogs, and cats from Rio de Janeiro, Brazil. The Faust et al. technique was used to examine fecal specimens collected in triplicate from 133 patients (52 humans, 60 dogs, and 21 cats). Specimens from 74 patients were received from the group assigned to carry out sampling on consecutive days - 34 humans, 35 dogs, and 5 cats, and specimens from 59 patients were received from the group assigned to carry out sampling on non-consecutive, separate days - 18 human beings, 25 dogs, and 16 cats. G. duodenalis cysts were found in stools of 30 individuals. Multiple stool sampling resulted in an increase in the number of samples that were positive for Giardia in both groups. The authors therefore conclude that multiple stool sampling increases the sensitivity of the Faust et al . technique to detect G. duodenalis cysts in samples from humans, cats and dogs.

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Section: Methodsmentioning

confidence: 99%

The influence of serial fecal sampling on the diagnosis of giardiasis in humans, dogs, and cats

Uchôa

Sudré

Macieira

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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“…In 1938, the original concentration by flotation with ZnSO 4 solution method was described [19]. Since then, several modifications have been introduced to it [19][20][21][22]. In the present study, we applied the recommendations from CLSI (2005) [6].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Three Parasitological Stool Examination Methods with the Formalin-Ethyl Acetate Procedure for the Diagnosis of Intestinal Parasites in Humans

Madriz-Elisondo

Galván-Ramírez

O-Carrasco

et al. 2020

IJTDH

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Aims: The objective was to compare the sedimentation spontaneous in tube technique (SSTT), the direct smear, the zinc sulfhate (surface film and sediment analyzed) methods with the formalin-ethyl acetate method as the reference standard. Study Design: This a cross-sectional study performed in two populations around Chapala lake, Jalisco, Mexico. Place and Duration of Study: Sample Department of Medical Sciences and Life, University Center of the Ciénega, University of Guadalajara, Ocotlán, Jalisco, Mexico. Department of Physiology, Health Sciences University Center. University of Guadalajara, Jalisco. México, between June 2018 and July 2019. Methodology: Sample: We included a total of 297 samples and were analyzed by the direct smear, the zinc sulfhate (surface film and sediment analyzed), the SSTT and formalin-ethyl acetate methods. Results: The SSTT was able to detect 40% of intestinal parasites, very good agreement for Entamoeba complex, Entamoeba coli, and Ascaris lumbricoides kappa index=0.697, 0.791, 0.696, respectively and excellent agreement for Giardia lamblia kappa index = 0.843. Regarding the isolation of Blastocystis spp, only a poor agreement was found among all techniques. The SSTT was able to detect multiple parasites with a sensitivity of 82.90% and specificity 83.80 kappa index= 0.649. Conclusion: The SSTT showed a very good agreement for the diagnosis intestinal polyparasitism which could represent another alternative for the concentration and identification protozoans and intestinal helminthes in low-resource settings.

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Método de Faust et al.: rendimento de colheita por alça metálica

Cited by 2 publications

References 0 publications

The influence of serial fecal sampling on the diagnosis of giardiasis in humans, dogs, and cats

The influence of serial fecal sampling on the diagnosis of giardiasis in humans, dogs, and cats

Comparison of Three Parasitological Stool Examination Methods with the Formalin-Ethyl Acetate Procedure for the Diagnosis of Intestinal Parasites in Humans

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