Eosinophil levels in the acute phase of experimental chagas' disease

Abstract

Eosinophil dynamics, in bone marrow, blood and peritoneal exudate, of resistant C57B1/6 (C57) and susceptible A/Snell (A/Sn) mice was comparatively studied during the acute phase of infection by Trypanosoma cruzi Y strain. A decline was observed in bone marrow eosinophil levels in A/Sn, but not in C57 mice, soon after infection, those of the former remaining significantly below those of the latter up to the 4th day of infection. Bone marrow eosinophil levels of C57 mice declined subsequently to levels comparable to those of A/Sn mice, the number of these cells in this compartment remaining 50% those of non infected controls, in both strains, up to the end of the experiment on the 14th day of infection. The fluctuations in eosinophil levels in blood and peritoneal space were similar in both mice strains studied. Concomitantly with depletion of eosinophils in the marrow, depletion in blood and a marked rise of these cells in the peritoneal space, initial site of infection, occurred in both strains. The difference in eosinophil bone marrow levels, between C57 and A/Sn mice, observed in the first four days of infection, suggests a higher eosinopoiesis capacity of the former in this period, which might contribute to their higher resistance to T. cruzi infection.

Different Infective Forms Trigger Distinct Immune Response in Experimental Chagas Disease

“…Nakhle et al (1989) [22] infected mice, A/Sn (susceptible) and C57BL/6 (resistant) with the Y strain of T. cruzi , and noted a decrease in eosinophils in bone marrow and the peripheral blood in A/Sn animals, which did not occur in C57BL/6 mice, suggesting a greater capacity for eosinophilopoiesis after infection in C57BL/6 mice that might contribute to its greater resistance to infection. Moreover, Rowland & Sibley-Phillips (1984) [23] found that the largest number of eosinophils in the bone marrow of C57BL/6 coincided with the parasitemia peak at 28 days after infection.…”

Congenital Transmission of Trypanosoma cruzi: A Review About the Interactions Between the Parasite, the Placenta, the Maternal and the Fetal/Neonatal Immune Responses

“…On the other hand, PRG2 a gene that encodes the precursor form of the eosinophil major basic protein (proMBP) is an upregulated DEG in T. cruzi -infected placentas. MBP can participate in the extracellular killing of parasites in the absence of antibodies (Nakhle et al, 2018). Another important function of MBP in the placenta is the inhibition of the pregnancy-associated plasma protein A (PAPPA) (Weyer and Glerup, 2011), whose low levels are associated with intrauterine growth restriction (Albu et al, 2014).…”

<i>Trypanosoma congolense</i>: Prophylactic Potentials Of Antiserum And Adjuvant In Experimental Mice

“…Similarly, Eosinophils are components of the immune system that fight multicellular parasites and are most often regarded as indicators of parasitic infections. Gleich and Adolph (1986) demonstrated, the participation of eosinophils in host defense mechanisms to helminth 27 infection and the in-vitro killing of various parasites by eosinophils from different species while Nakhle et al, (1989) In this study, we did not monitor antibodies that induce immunosuppression in the course of the infection. However, the differential WBC counts can give an idea with regard the immune responses observed in the groups of mice immunized with the antiserum.…”