Susceptibilidad "in vitro" de cepas de Cryptococcus a 5 drogas antifungicas

Abstract

Se estudió la susceptibilidad "in vitro" de 24 cepas de 3 especies del género Cryptococcus a 5 drogas antifúngicas (anfotericina B, 5 fluorocitosina, ketoconazol, itraconazol y miconazol). Las mismas se agruparon según su especie, variedad y origen de aislamiento. Para determinar la concentración inhibitoria mínima (C.I.M.) de cada droga se empleó el método de dilución en agar con el medio básico nitrogenado para levaduras, adicionado de glucosa. Se obtuvo además la media geométrica de estos valores para cada grupo y se comparó cada uno de ellos. Los resultados obtenidos fueron homogéneos con la sola excepción de las cepas de Cryptococcus sp (no neoformans), en las cuales se detectaron elevados valores de C.I.M. para la 5 fluorocitosina.

Theoretical and experimental characterization of a novel pyridine benzimidazole: suitability for fluorescence staining in cells and antimicrobial properties

“…During the course of this study, we realized that 2,4-di-tertbutyl-6-(3H-imidazo [4,5-c]pyridine-2-yl)phenol (B2) remained somehow ''attached'' to cells, retaining its luminescence. When Salmonella enterica were mixed with B2, we observed that bacteria remained fluorescent even after 4 washes with phosphate buffer solution (PBS) 83 (Fig. 11A).…”

Spectral, theoretical characterization and antifungal properties of two phenol derivative Schiff bases with an intramolecular hydrogen bond

“…strains usually analyzed. [71][72][73][74][75][76] Interestingly, L2 presented a noteworthy antifungal activity against the same strain of Cryptococcus spp. (MIC: 4.468 mg ml À1 ), strongly suggesting that L2 exerts its antifungal effect through a different mechanism compared with K1.…”

In vitro susceptibilities of clinical and environmental isolates of Cryptococcus neoformans to five antifungal drugs

“…Few studies on the antifungal susceptibilities of clinical and environmental strains of C. neoformans have been performed (3,9), especially in Brazil, where little is known about the occurrence and characterization of the yeast (15,22). The purpose of the current investigation was to assess the in vitro susceptibilities of clinical and environmental isolates of C. neoformans to five antifungal drugs by using the reference macrodilution method proposed by the National Committee for Clinical Laboratory Standards (NCCLS) (18).…”