Glicolipoproteína de Leptospira interrogans sorogrupo icterohaemorrhagiae: distribuição em fígado e rim de cobaias experimentalmente infectadas

Santos, R. T. M.; Sakata, Elena Emiko; Yasuda, Paulo Hideki; Wakamatsu, Alda; Kanamura, Cristina Takami; Candelori, Ignez; Pestana, C B; Alves, Venâncio Avancini Ferreira

doi:10.1590/s0036-46651989000400005

Cited by 4 publications

(4 citation statements)

References 20 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other toxic effects exerted by GLP may be observed in cultures of Vero and L929 cells, leading to cell membrane perforations followed by cell death (43). The presence of GLP in damaged tissues of patients and experimental animals further supports the role of this molecule in leptospirosis (25,33).…”

Section: Discussionmentioning

confidence: 74%

“…This may explain some electrolytic alterations observed in leptospirosis patients with acute renal failure (1,26,36). In experimental models with guinea pigs injected with pathogenic L. interrogans, GLP can be detected in the damaged tissues adhering to endothelial cells and to epithelial membranes, accompanying other antigen deposits resulting from bacterial destruction by the immune system (3,25). Therefore, the GLP complex may contain toxins potentially involved in the pathogenesis of leptospirosis.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Peripheral Blood Mononuclear Cell Activation Induced byLeptospira interrogansGlycolipoprotein

et al. 2002

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 74%

mentioning

confidence: 99%

Peripheral Blood Mononuclear Cell Activation Induced byLeptospira interrogansGlycolipoprotein

et al. 2002

View full text Add to dashboard Cite

show abstract

“…The purpose of this study was to develop a test with a candidate antigen, namely Glycolipoprotein (GLP), for a new serodiagnostic strategy. The GLP is an antigenic complex which contains toxins involved in the pathogenesis of leptospirosis (Macedo‐Santos et al. 1989; Diament et al.…”

Section: Introductionmentioning

confidence: 99%

Leptospiral glycolipoprotein as a candidate antigen for serodiagnosis of human leptospirosis

Blanco

Takei

Romero

2009

Letters in Applied Microbiology

View full text Add to dashboard Cite

Aims: Development of a simple, specific, rapid and inexpensive Dot‐ELISA test for early diagnosis of human leptospirosis. Methods and Results: Serum samples from 90 patients diagnosed with leptospirosis were analysed by Dot‐ELISA test incorporating Glycolipoprotein (GLP) antigen from serovars Copenhageni and Patoc. Results were compared with those obtained with microscopic agglutination test, currently, the gold standard reference serological method. Serum samples from healthy blood bank donors and patients diagnosed with diseases other than leptospirosis were used as negative controls. The specificities of both GLP‐based assays were 97·1% and 100% with serum samples from patients with other diseases and with serum samples from healthy control group, respectively. With serum samples from patients with acute leptospirosis, sensitivity was 76·6% with Dot‐ELISA Copenhageni and 90·0% with Dot‐ELISA Patoc. With serum samples from patients in convalescence, sensitivity was 100% with both GLP‐based assays. Conclusions: This Dot‐ELISA provides a candidate antigen for serodiagnosis of leptospirosis during all phases of illness and could be a good alternative method for the early diagnosis of leptospirosis. Significance and Impact of the Study: The Dot‐ELISA test is simple, specific, rapid and inexpensive. It is suitable for identifying a large number of samples and, hence, reducing the death rate of patients with leptospirosis.

show abstract

“…GLP is likely to be involved in the pathogenesis of the disease as suggested by the presence of GLP in damaged tissues of patients and experimental animals (19). Moreover, it was demonstrated that this component might block the tubular epithelial cell sodium pump in the kidneys (10).…”

Section: Introductionmentioning

confidence: 99%

Leptospira interrogans activation of peripheral blood monocyte glycolipoprotein demonstrated in whole blood by the release of IL-6

Dorigatti

Brunialti

Romero

et al. 2005

Braz J Med Biol Res

View full text Add to dashboard Cite

Glycolipoprotein (GLP) from pathogenic serovars of Leptospira has been implicated in the pathogenesis of leptospirosis by its presence in tissues of experimental animals with leptospirosis, the inhibition of the Na,K-ATPase pump activity, and induced production of cytokines. The aims of the present study were to investigate the induction of IL-6 by GLP in peripheral blood mononuclear cells (PBMC) and to demonstrate monocyte stimulation at the cellular level in whole blood from healthy volunteers. PBMC were stimulated with increasing concentrations (5 to 2500 ng/ml) of GLP extracted from the pathogenic L. interrogans serovar Copenhageni, lipopolysaccharide (positive control) or medium (negative control), and supernatants were collected after 6, 20/24, and 48 h, and kept at -80ºC until use. Whole blood was diluted 1:1 in RPMI medium and cultivated for 6 h, with medium, GLP and lipopolysaccharide as described above. Monensin was added after the first hour of culture. Supernatant cytokine levels from PBMC were measured by ELISA and intracellular IL-6 was detected in monocytes in whole blood cultures by flow-cytometry. Monocytes were identified in whole blood on the basis of forward versus side scatter parameters and positive reactions with CD45 and CD14 antibodies. GLP (≥50 ng/ml)-induced IL-6 levels in supernatants were detected after 6-h incubation, reaching a peak after 20/24 h. The percentage of monocytes staining for IL-6 increased with increasing GLP concentration. Thus, our findings show a GLPinduced cellular activation by demonstrating the ability of GLP to induce IL-6 and the occurrence of monocyte activation in whole blood at the cellular level.

show abstract

Glicolipoproteína de Leptospira interrogans sorogrupo icterohaemorrhagiae: distribuição em fígado e rim de cobaias experimentalmente infectadas

Cited by 4 publications

References 20 publications

Peripheral Blood Mononuclear Cell Activation Induced byLeptospira interrogansGlycolipoprotein

Peripheral Blood Mononuclear Cell Activation Induced byLeptospira interrogansGlycolipoprotein

Leptospiral glycolipoprotein as a candidate antigen for serodiagnosis of human leptospirosis

Leptospira interrogans activation of peripheral blood monocyte glycolipoprotein demonstrated in whole blood by the release of IL-6

Contact Info

Product

Resources

About