Utilidad de las muestras de sangre total desecada en papel de filtro en la detección de anticuerpos antitoxoplasma

Se ha estudiado la prevalência de anticuerpos antitoxoplasma en dos comunidades rurales rwandesas, utilizando sangre total desecada en papel de filtro que se procesó por la técnica de Aglutinación Directa. En ambas comunidades están afectados el 50% de los adultos. La adquisición de los anticuerpos se hace tardiamente en NGD (a los 14 años sólo un 12% de la problación muestra anticuerpos antitoxoplasma) y más pronto en NVU (31% de la población estudiada tiene anticuerpos antitoxoplasma a los 14 años). Se destaca el posible papel que juega esta enfermedad en la patología materno-fetal, y la necesidad de nuevos studios que aumenten el conocimiento de la epidemiología de la toxoplasmosis y sus mecanismos de transmisión en Rwanda.

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Sero-epidemiologia de la toxoplasmosis en dos comunidades de Rwanda (Africa Central)

Gascón¹,

Torres-Rodríguez

Soldevila³

et al. 1989

Rev. Inst. Med. trop. S. Paulo

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Detection by Enzyme Immunosorbent Assay of Toxoplasma gondii IgG Antibodies in Dried Blood Spots on PKU-filter Paper from Newborns

Lebech

Petersen

1995

Scandinavian Journal of Infectious Diseases

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Different ways of eluting IgG antibodies against Toxoplasma gondii from blood spots on PKU filter papers from newborns were examined, and the eluate was analysed by Enzyme Immunosorbent Assay (EIA). 10-mm diameter discs (method A), and 3-mm discs (method B) were eluted overnight, and 3-mm discs were eluted directly for 2 hours in the EIA wells (method C). Two groups of paired samples were tested: blood spots on the PKU card from the newborn, cord blood, and a venous blood sample from the mother collected shortly after delivery (Group 1, 191 samples); or PKU cards from the newborns and serum collected from the mother during the first trimester (Group 2, 426 samples). The best results were found for Group 1; method A, a sensitivity of 100% (63/63), and a specificity of 100% (128/128) (r = 0.934, P < 0.0001), and for Group 2; method C, a sensitivity of 100% (125/125), and a specificity of 100% (301/301) (r = 0.822, P < 0.0001). When method C, was used on 188 samples stored for 16-18 month at -20 degrees C, a sensitivity of 100% (62/62) and a specificity of 99.2% (125/126) (r = 0.913, P < 0.0001) were found. The PKU filter paper method is reliable and reproducible, and can be used in screening projects based on analysis of neonatal samples, and in epidemiological studies on toxoplasmosis in pregnant women.

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Utilidad de las muestras de sangre total desecada en papel de filtro en la detección de anticuerpos antitoxoplasma

Cited by 2 publications

References 3 publications

Sero-epidemiologia de la toxoplasmosis en dos comunidades de Rwanda (Africa Central)

Sero-epidemiologia de la toxoplasmosis en dos comunidades de Rwanda (Africa Central)

Detection by Enzyme Immunosorbent Assay of Toxoplasma gondii IgG Antibodies in Dried Blood Spots on PKU-filter Paper from Newborns

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