Medical Laboratory Manual for Tropical Countries. v. II: Microbiology

Re, McCall; Hb, Lessenberry

doi:10.1590/s0036-46651985000400013

Cited by 4 publications

(5 citation statements)

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“…In developing countries, intestinal parasitic infections remain a significant cause of mortality and morbidity 1,2 . Parasitic infections can cause growth delay, iron deficiency anaemia, especially in children, and other psychological and physical health conditions 3 .…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of intestinal parasites among food handlers attending public health laboratories in Khartoum State, Sudan

et al. 2018

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Background: Infections by intestinal pathogens especially protozoans and helminths are considered to pose a real health problem, particularly in the tropics. They cause considerable morbidity and mortality rates in developing countries. The high prevalence of these infections is closely correlated with poverty, poor environmental hygiene, and impoverished health services. This study aimed to detect prevalence and frequency of parasitic infections among food handlers in Khartoum Sudan. Methods: Three hundred and fifty Food-handlers, attending public health laboratories in Khartoum, Sudan, for an annual medical check-up, were screened for intestinal parasites by four laboratory techniques viz. direct faecal examination, formal-ether concentration, Baermann technique and agar culture method. Results: The infection rate was 23.7% by Formol-Ether Concentration technique, followed by direct saline stool preparation (7.1%). Out of 83 positive samples the infection rate among different nationalities was as follows: Sudanese 68 (81.9%), Ethiopians 13 (15.7%), Syrians 2 (2.4%) and Egyptians 0 (0%). Intestinal parasites were more prevalent among males (73; 25.1%) than female food handlers (10; 16.9%). Three protozoans, nematodes, two tap worms and one trematode worm were detected among infected population: their frequency were as follows: Entamoeba histolytica (7.4%), Entamoeba coli (6.86%), Giardia lamblia (6%), Schistosoma mansoni (1.40%), Necator americanus (1.43%), Hymenolepis nana (0.68%), Strongyloides stercoralis (0.68%), Taenia saginata (0.57%), Ascaris lumbricoides (0.57%) and Trichostrongylus species (0.29%). Conclusion: The overall prevalence of protozoan infections among food handler in Khartoum state, Sudan was 20.26% while the helminthic infections was 5.97%. Formol-ether concentration technique is better for detection of intestinal parasites than the direct faecal smear technique. Likewise, Barmann’s technique confirms detection of nematodes worms especially hookworms.

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Section: Introductionmentioning

confidence: 99%

“…Soil-transmitted helminth (STH) and other helminth parasites like Taenia saginata and Hymenolepis nana, represent important causative agents of gastrointestinal infections. Intestinal protozoan dwellers, mainly Giardia lamblia and Entamoeba histolytica, also, contribute to intestinal disorders 1 .…”

Section: Introductionmentioning

confidence: 99%

Prevalence of intestinal parasites among food handlers attending public health laboratories in Khartoum State, Sudan

et al. 2018

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“…It was reported by Radwan et al (2021) that E. coli was isolated. The gathered specimens were introduced into MacConkey's broth and subjected to an aerobic 24-hour incubation period at 37 C. After that, MacConkey's agar was streaked with a loopful of broth cultures, and the mixture was aerobically incubated for 24 to 48 hours at 37 0 C. After being inoculated onto eosin methylene blue (EMB) agar medium, the lactose fermenter (pink) colonies were cultured for 18 to 24 hours at 37 0 C. According to Edward and Ewing (1972), suspected colonies of E. coli were inspected for morphological and cultural traits before being picked up and streaked into nutrient agar slopes for biochemical identi cation (Cheesbrough 1985). This biochemical identi cation was done using Oxidase, Catalase, Indole, Citrate, Methyl red, Voges Proskauer, Haemolysis on Blood Agar, and Urea utilization.…”

Section: Bacteriological Isolation and Identi Cation Of Ecolimentioning

confidence: 99%

Improving growth performance, immunity, and health status of rabbits by using cinnamon Nanoparticles

Tahoon,

Ali,

Naena

et al. 2024

Preprint

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The goal of this study was to find out how supplementing rabbits with cinnamon Nanoparticles (CNP) affected their immunity, growth performance, serum biochemistry, blood haematology, and the histology of their liver, lung, and kidney in addition to their protective impact against a fake infection with Escherichia coli O25. After being acclimated for two weeks and separated into six equal groups, sixty weaned white New Zealand rabbits (35 days old) that appeared healthy were acquired from a private farm in Kafr EL-Sheikh Governorate. According to our findings, the rabbits that got CNP exhibited better growth performance, P.M. lesions, bacterial re-isolation outcomes, and clinical symptoms. The hepatic and renoprotective effects of CNP may be supported by the mean values of serum ALT and AST levels decreasing and mean values of total protein, albumin, globulin, total white blood cell count, lymphocyte count, and antioxidant enzymes (SOD and CAT) increasing. The histological analysis showed that the positive control group had apparent pathological changes, such as interstitial nephritis, hepatic coagulative necrosis, and interstitial pneumonia with mononuclear cell infiltrations. The CNP and streptomycin-treated group, on the other hand, showed milder to moderately changed tissue conditions. It is possible to conclude that CNP can be used as an alternative to antibiotics because the combined treated group showed high efficacy (growth performance, antibacterial, and favourable effects on liver, kidney, and antioxidant enzyme functions in addition to reducing the histological alteration changes in liver, kidney, and lung tissues).

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“…Plates were incubated at 37 0 C for 24-48 hours under aerobic condition. From the pure culture bacterial agents were identified by studying colony characteristics, Gram staining reaction, hemolysis pattern and biochemical test as described by Merchant and Packer (1967) and Cheesbrough (1984).…”

Section: Isolation and Identification Of Bacterial Agentsmentioning

confidence: 99%

Antibiotic resistance and residue in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh

Matubber

Rume

Kayesh

et al. 2021

Asian Australas. J. Food. Saf. Secur

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The presence of antibiotic residue in chicken and animal meats is a serious threat to human health due to its harmful effects. This study aimed at identifying the antibiotic resistance patterns of the isolates as well as antibiotic residues in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh. A total of 205 meat samples, including 70 chicken meat, 60 cattle meat, 50 buffalo meat and 25 goat meat were aseptically collected and analysed for the detection of antibiotic residues by thin layer chromatography and the isolates obtained from these samples were subjected to antibiogram study against 16 commonly used antibiotics. The isolates found in this study were Staphylococcus spp., Streptococcus spp., Escherichia coli, and Salmonella spp. and their prevalence were 37.5% (77/205), 22.1% (48/205), 29.7% (61/205), 8.7% (19/205), respectively. The isolates showed different degrees of sensitivity to the antibiotics used in the study. The most resistant phenotype was against cefradine, amoxicillin, penicillin, oxytetracycline, erythromycin, and enrofloxacin. 18.5% (38/205) meat samples were found to be positive for antibiotic residues and the highest prevalence was observed in chicken meat compared to other meat types. Overall, the findings of the study suggest that it is important to take controlling measures for the emergence of antibiotic resistance and also for ensuring healthy meats for human consumption. Asian Australas. J. Food Saf. Secur. 2021, 5 (1), 19-26

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Medical Laboratory Manual for Tropical Countries. v. II: Microbiology

Cited by 4 publications

References 0 publications

Prevalence of intestinal parasites among food handlers attending public health laboratories in Khartoum State, Sudan

Prevalence of intestinal parasites among food handlers attending public health laboratories in Khartoum State, Sudan

Improving growth performance, immunity, and health status of rabbits by using cinnamon Nanoparticles

Antibiotic resistance and residue in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh

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