Uma tentativa de cultivo de rotavírus humano em cultura de leucócitos humano (Nota Prévia)

Nozawa, Carlos; Fonseca, M. E. F.

doi:10.1590/s0036-46651984000400008

Rev. Inst. Med. trop. S. Paulo

1984

DOI: 10.1590/s0036-46651984000400008

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Uma tentativa de cultivo de rotavírus humano em cultura de leucócitos humano (Nota Prévia)

Carlos Nozawa

M. E. F. Fonseca

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1987

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Cited by 4 publications

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Cultivation of avian rotaviruses in chicken lymphocytes and lymphoblastoid cell lines

Schat

Myers

1987

Archives of Virology

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Avian rotavirus isolates were used to infect normal chicken spleen cells, lymphoblastoid T cell lines transformed by Marek's disease virus, an avian leukosis virus-transformed B cell line, and a reticuloendotheliosis virus-transformed line, which is a pre-B, pre-T cell line. All five isolates tested were able to infect spleen cells and the three types of lymphoblastoid cell lines, suggesting that avian rotaviruses can infect both B and T cells. Splenic lymphocytes were considerably less susceptible to infection than chick kidney cells. Lymphoblastoid cell lines remained virus-positive during a 10-day culture period. Virus was neutralized by the addition of low dilutions of normal chicken serum and high dilutions of chicken anti-rotavirus serum.

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Cultivation of avian rotaviruses in chicken lymphocytes and lymphoblastoid cell lines

Schat

Myers

1987

Archives of Virology

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Standardization and kinetics of in vitro bovine blood lymphocyte stimulation with bovine rotavirus

Archambault

Morin

Elazhary

et al. 1988

Comparative Immunology, Microbiology and Infectious Diseases

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Two groups of 3-month old calves were immunized intramuscularly with attenuated bovine rotavirus and boosted 21 and 42 days later. The first group of three calves were vaccinated with live virus emulsified with incomplete Freund's adjuvant (IFA) and the second group was immunized with live virus suspended in phosphate buffered saline (PBS). Three other calves, serving as controls, were inoculated with PBS emulsified with IFA. The specific cell-mediated and antibody responses of the animals were studied. Preliminary analysis of in vitro peripheral blood lymphocyte transformation to bovine rotavirus determined optimal conditions as: 96 h culture period, 5 X 10(5) cells per culture in RPMI 1640 medium containing 10% heat-inactivated bovine fetal serum and the use of inactivated virus in the cell culture at a concentration of 5 X 10(6) median tissue culture infective dose before inactivation. Specific blastic stimulation was observed on calves immunized with the rotavirus emulsified with IFA after the second and third vaccine inoculation with stimulation index values varying from 2.00 to 5.73. Serum neutralizing antibody titers of 1/25,600 were also induced in the same calves. Calves immunized with rotavirus-PBS suspension developed a mean antibody titer of 1/1,600, but showed no specific lymphocyte stimulation. No increase in specific immune responses was detected in the control animals.

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Extraintestinal rotavirus infections in children with immunodeficiency

Gilger

Matson

Conner

et al. 1992

The Journal of Pediatrics

157

102

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Uma tentativa de cultivo de rotavírus humano em cultura de leucócitos humano (Nota Prévia)

Cited by 4 publications

References 3 publications

Cultivation of avian rotaviruses in chicken lymphocytes and lymphoblastoid cell lines

Cultivation of avian rotaviruses in chicken lymphocytes and lymphoblastoid cell lines

Standardization and kinetics of in vitro bovine blood lymphocyte stimulation with bovine rotavirus

Extraintestinal rotavirus infections in children with immunodeficiency

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