Inquérito soroepidemiológico sobre malária em escolares de Marabá, Pará

Boulos, Marcos; Ceneviva, Antonio Carlos; Shiroma, M; Camargo, M. E.; Castilho, Euclides Ayres de

doi:10.1590/s0036-46651984000100009

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…is very evident. Animal Plasmodium antigens are commonly used in making serodiagnosis of human malaria because of cross-reactions between homologous species of Plasmodium (BOULOS et al, 1984). Even though some authors have stated that serodiagnosis can be used as a screening test to confirm exposure to current or former malaria infection (NARDIN et al, 1989;VOLNEY et al, 2002), the responses are often less expressive, even in endemic areas, and thus the degree of cross-reaction is also low.…”

Section: Discussionmentioning

confidence: 99%

Serological and molecular techniques applied for identification of Plasmodium spp. in blood samples from nonhuman primates

Figueiredo

Santi

Manrique

et al. 2018

Rev. Bras. Parasitol. Vet.

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The aim of this study was to identify Plasmodium spp. in blood samples from nonhuman primates (NHPs) in the state of Maranhão, using classical and alternative techniques for examination of human malaria. A total of 161 blood samples from NHPs were analyzed: 141 from captive animals at a Wildlife Screening Center (CETAS) and 20 from free-living animals in a private reserve. The techniques used were microscopy, rapid diagnostic test (RDT), Indirect fluorescent antibody test (IFAT) and molecular techniques (semi-nested PCR, quantitative real-time PCR and LAMP). Two serological methods (dot-ELISA and indirect ELISA) were also standardized with rhoptry protein-soluble antigen of P. falciparum and P. berghei. Trophozoite forms of Plasmodium sp. were identified on slides from five different animals. No samples were positive through RDT and LAMP. Four samples were seropositive for P. malariae through IFAT. The samples showed low reactivity to ELISA. Plasmodium sp. was detected in 34.16% (55/161) of the samples using qPCR based on the 18S rRNA gene. After sequencing, two samples showed 100% identityl to P. malariae, one showed 97% identity to Plasmodium sp. ZOOBH and one showed 99% identity to P. falciparum . PCR was shown to be the most sensitive technique for diagnosing Plasmodium in NHP samples.

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Section: Discussionmentioning

confidence: 99%

Serological and molecular techniques applied for identification of Plasmodium spp. in blood samples from nonhuman primates

Figueiredo

Santi

Manrique

et al. 2018

Rev. Bras. Parasitol. Vet.

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“…falciparum and P . berghei antigens are justified, since cross-reactivity is expected, given that it is known that Plasmodium antigens produced in laboratories (especially from animals) are usually used in making the serodiagnosis of human malaria [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil

Figueiredo

Santi²,

Manrique

et al. 2017

PLoS ONE

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In the Brazilian Amazon region, malaria caused by Plasmodium malariae is considered to be a zoonosis because of cross-transfer of the parasite between humans and Neotropical primates. To contribute information on this issue, we investigated occurrences of natural infection with Plasmodium sp. among Neotropical primates in the Maranhense Amazon (Amazon region of the state of Maranhão), in the northeastern region of Brazil. Blood samples were collected from 161 Neotropical primates of six species that were caught in an environmental reserve (Sítio Aguahy) and from captive primates (CETAS—Wildlife Screening Center, municipality of São Luís), in Maranhão. Plasmodium sp. was diagnosed based on light microscopy, PCR, qPCR and LAMP for amplification of the 18S rRNA gene. Serum samples were also assayed by means of indirect immunofluorescence for IgG antibodies against P. malariae/P. brasilianum, P. falciparum and P. berghei. Parasites were detected through light microscopy on five slides from captive primates (four Sapajus spp. and one Callithrix jacchus). In the molecular tests, 34.16% (55/161) and 29.81% (48/161) of the animals sampled were positive in the qPCR and PCR assays, respectively. In the PCR, 47/48 animals were positive for P. malariae/P. brasilianum; of these, eight were free-living primates and 39 from CETAS, São Luís. One sample showed a band in the genus-specific reaction, but not in the second PCR reaction. Anti-P. malariae/P. brasilianum IgG antibodies were detected in four serum samples from Sapajus spp. in captivity. In this study, circulation of P. malariae/P. brasilianum in Neotropical primates was confirmed, with low levels of parasitemia and low levels of antibodies. The importance of these animals as reservoirs of human malaria in the region studied is still unknown. This scenario has an impact on control and elimination of malaria in this region.

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Inquérito soroepidemiológico sobre malária em escolares de Marabá, Pará

Cited by 2 publications

References 8 publications

Serological and molecular techniques applied for identification of Plasmodium spp. in blood samples from nonhuman primates

Serological and molecular techniques applied for identification of Plasmodium spp. in blood samples from nonhuman primates

Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil

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