Paridade de Ochlerotatus scapularis em condições de laboratório e campo

Deus, Juliana Telles de; Kakitani, Iná

doi:10.1590/s0034-89102006000300020

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…The epidemiological importance of this mosquito vector is reinforced by its gonotrophic discordance: as females might need more than one blood repast during a single gonotrophic cycle, this increases their contact with possible hosts [14]. …”

Section: Introductionmentioning

confidence: 99%

High morphological and genetic variabilities of Ochlerotatus scapularis, a potential vector of filarias and arboviruses

Petersen

Devicari

Suesdek

2015

Parasites & Vectors

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BackgroundOchlerotatus scapularis is a potential vector of filarias and arboviruses in the Neotropics. This species was once typically associated with sylvatic environments; however, cases of synanthropy and urbanization of this species have been increasingly reported in southeast Brazil. Despite the medical relevance of Oc. scapularis, its populational variability is not yet known. To our knowledge, this is the first report describing the morphological and genetic variabilities of this species.MethodsPopulation samples were characterized using the cytochrome oxidase subunit I (COI) mitochondrial gene and wing geometrics. Adult mosquitoes were collected from five sampling sites from remnants of the Atlantic forest embedded in the urban or rural areas of southeast Brazil.ResultsIn the 130 individuals analyzed, 46 COI haplotypes were detected. Haplotype diversity was high and ranged from 0.66 to 0.97. Six haplotypes were present in 61% of the individuals, whereas the remaining haplotypes were less frequent (39%). Wing shape was also highly polymorphic. Differentiation of populations across sampling sites according to genetic distances (Fst = −0.009 to 0.060) and morphological distances (Qst = 0.47) indicated that populations were not identical. No correlations were noted for phenetic and genetic diversities (p = 0.19) or for genetic or phenetic distances with geographical distances (p = 0.2 and p = 0.18, respectively).ConclusionsOur study results suggest that Oc. scapularis has a rich genetic patrimony, even though its habitat is fragmented. Implications of such genetic richness with respect to vectorial competence, plasticity, and ability to exploit urbanized areas need to be further investigated.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-0740-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

High morphological and genetic variabilities of Ochlerotatus scapularis, a potential vector of filarias and arboviruses

Petersen

Devicari

Suesdek

2015

Parasites & Vectors

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Parity and gonotrophic discordance of females ofAedes aegyptiandAedes albopictus(Diptera: Culicidae) in the city of São Paulo, SP, Brazil

Andrade

Urbinatti

Coelho

et al. 2019

Journal of Vector Ecology

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The objective of this study was to assess the parity, presence of blood in the stomach, and the gonotrophic discordance of females of Aedes aegypti and Aedes albopictus captured in two areas of the city of São Paulo. The captures were undertaken monthly, by aspiration, in the period from January, 2015 to August, 2017. All the females of the two species had their midguts and ovaries dissected to determine the presence of blood and the parity/stage of maturation. With regard to parity, 27% and 34% of the females of Ae. aegypti and Ae. albopictus, respectively, were parous or were in advanced stages of the development of their ovaries (33% and 27%, respectively). The larger part of the females of Ae. aegypti and Ae. albopictus contained blood in their stomachs (77% and 60%, respectively), beyond which 36% and 27% of the females of Ae. aegypti and Ae. albopictus, respectively, were in gonotrophic discordance. Our results indicate favorable conditions in the study areas because of the presence of parous females. Moreover, this frequent and multiple contact of Ae. aegypti and Ae. albopictus females with vertebrate hosts, such as humans, increases the possibility of transmitting the viruses they may be carrying.

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Ilheus Virus Isolation in the Pantanal, West-Central Brazil

et al. 2013

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The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolândia sub-region of Pantanal reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009. To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region. Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of 2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The present data confirm the circulation of ILHV in the Brazilian Pantanal.

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Paridade de Ochlerotatus scapularis em condições de laboratório e campo

Cited by 4 publications

References 13 publications

High morphological and genetic variabilities of Ochlerotatus scapularis, a potential vector of filarias and arboviruses

High morphological and genetic variabilities of Ochlerotatus scapularis, a potential vector of filarias and arboviruses

Parity and gonotrophic discordance of females ofAedes aegyptiandAedes albopictus(Diptera: Culicidae) in the city of São Paulo, SP, Brazil

Ilheus Virus Isolation in the Pantanal, West-Central Brazil

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