Detection of pathogens from periodontal lesions

Malheiros, Veruska de João; Ávila-Campos, Mário Júlio

doi:10.1590/s0034-89102004000500016

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…In this study, by culture, A. actinomycetemcomitans was isolated from 18% of the periodontal patients and 2% of the healthy subjects, in accordance with Asikainen et al (1) and Malheiros and AvilaCampos (22). Moreover, studies have shown that the occurrence of A. actinomycetemcomitans varied from 20% to 80% in adult periodontitis patients (3,22,33). On the other hand, by using a PCR method, this microorganism was detected in 44% of the periodontal patients, and in 16% of the healthy subjects.…”

Section: Discussionsupporting

confidence: 87%

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Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis

Gaetti‐Jardim¹,

Wahasugui²,

Tomazinho³

et al. 2008

Braz. J. Microbiol.

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Aggregatibacter actinomycetemcomitans is an important etiologic agent of the periodontitis and is associated with extra-oral infections. In this study, the detection of the ltxA gene as well as the ltx promoter region from leukotoxic A. actinomycetemcomitans isolated from 50 Brazilian patients with periodontitis and 50 healthy subjects was performed. The leukotoxic activity on HL-60 cells was also evaluated. Leukotoxic activity was determined using a trypan blue exclusion method. The 530 bp deletion in the promoter region was evaluated by PCR using a PRO primer pair. A. actinomycetemcomitans was detected by culture and directly from crude subgingival biofilm by PCR using specific primers. By culture, A. actinomycetemcomitans was detected in nine (18%) of the periodontal patients and one (2%) healthy subject. However, by PCR, this organism was detected in 44% of the periodontal patients and in 16% of the healthy subjects. It was verified a great discrepancy between PCR detection of the ltx operon promoter directly from crude subgingival biofilm and from bacterial DNA. Only one periodontal sample harbored highly leukotoxic A. actinomycetemcomitans. Moreover, biotype II was the most prevalent and no correlation between biotypes and leukotoxic activity was observed. The diversity of leukotoxin expression by A. actinomycetemcomitans suggests a role of this toxin in the pathogenesis of periodontal disease and other infectious diseases.

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Section: Discussionsupporting

confidence: 87%

“…In healthy subjects, biotypes II, VI and X were observed. Few studies have shown the presence of biotypes in A. actinomycetemcomitans and the biotype II appears to be the most prevalent in Brazilian periodontal patients (3,22).…”

Section: Discussionmentioning

confidence: 99%

Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis

Gaetti‐Jardim¹,

Wahasugui²,

Tomazinho³

et al. 2008

Braz. J. Microbiol.

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“…Those pathogens found in Brazilian patients with periodontal diseases include A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia, C. rectus, Eikenella corrodens, Treponema denticola, Streptococcus spp., and some others (3,4,6,7,8,9,24,26,32,33,34). In particular, these previous studies focused on the prevalence of periodontal pathogens in diseased subjects representing four different stages of clinical treatment, as follows: (i) before periodontal treatment, (ii) after nonsurgical mechanical therapy (scaling and root planing), (iii) after antibiotic therapy, and (iv) after surgical treatment.…”

Section: Discussionmentioning

confidence: 99%

Etiological Analysis of Initial Colonization of Periodontal Pathogens in Oral Cavity

et al. 2008

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It is unclear when the initial colonization by periodontal pathogens occurs in the oral cavity. Therefore, we report here the association between specific age groups and the time when the initial colonization by periodontal pathogens occurs in the oral cavity in such groups. Findings are based on an epidemiological analysis of the prevalence of five periodontal pathogens in the oral cavities of a wide range of age populations, from newborn to elderly, who were randomly selected in a geographic region of Brazil. These periodontal pathogens include Campylobacter rectus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Tannerella forsythia and were analyzed in the bacterial samples isolated from gingival sulcus, the dorsum of the tongue, and cheek mucosa of diverse age groups, using a bacterial DNA-specific PCR method. Results indicated that there are distinct age-related groups where initial colonization by the five periodontal pathogens examined in this study can be detected and that the presence of teeth is a permissive factor for colonization by P. gingivalis, P. intermedia, and T. forsythia. Although it remains unclear exactly how or when target pathogens colonize healthy subjects, an understanding of age-related groups does provide a potentially useful tool in the early detection and prevention of periodontitis in healthy individuals.

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“…The prevalence of A. actinomycetemcomitans in aggressive periodontitis was found to range between 67% and 80%, with the majority of isolates belonging to biotype X . In chronic periodontitis, the prevalence of A. actinomycetemcomitans periodontitis was 18% with a predominance of biotype II . Leukotoxic strains of A. actinomycetemcomitans (JP2 clone, serotype b) were a common finding in aggressive periodontitis in South America, with a particularly high prevalence in Brazil .…”

Section: Periodontal Microbiology In Latin Americamentioning

confidence: 96%

Periodontal microbiology in Latin America

Contreras¹,

Moreno²,

Jaramillo³

et al. 2014

Periodontology 2000

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This review article describes the microbiota associated with periodontal disease in Latin America. This vast territory includes 22 nations, which show great ethnic diversity, with large groups of White people, Black people, Mestizo people and Native people. Widespread poverty and limited access to education and health-care services, including periodontal care, are prominent predisposing factors for destructive periodontal disease in Latin America. Black people and Mestizo people seem to have particularly severe periodontal disease and are frequently colonized by the major periodontal pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. The 'red complex' bacterial pathogens and A. actinomycetemcomitans predominate in chronic and aggressive periodontitis, but gram-negative enteric rods and herpesviruses can also play important periodontopathic roles in Latin America. The key to minimizing the risk of periodontal disease is control of the pathogens, and new low-cost periodontal treatments deserve serious consideration in Latin America.

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Detection of pathogens from periodontal lesions

Cited by 6 publications

References 16 publications

Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis

Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis

Etiological Analysis of Initial Colonization of Periodontal Pathogens in Oral Cavity

Periodontal microbiology in Latin America

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