Influência de extratos de formas evolutivas sobre atividades de oviposição de fêmeas de Aedes (s) albopictus (Skuse)

Marques, Cristiano C.A.; Miranda, C. O.

doi:10.1590/s0034-89101992000400009

Cited by 6 publications

(9 citation statements)

References 6 publications

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“…These findings are somewhat consistent with earlier preliminary experiments showing that extracts from Ae. albopictus larvae and pupae (but not eggs) were active to conspecific gravid females 10 . We next tested extracts from Cx.…”

Section: Resultsmentioning

confidence: 97%

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Conspecific and allospecific larval extracts entice mosquitoes to lay eggs and may be used in attract-and-kill control strategy

Faierstein

Sena

et al. 2019

Sci Rep

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One of the strategies of integrated vector management is to lure gravid mosquitoes for surveillance purposes or to entice them to lay eggs in water containing toxins that kill the offspring (attract-and-kill or trap-and-kill). Typically, the major challenge of this approach is the development of a lure that stimulates oviposition plus a toxin with no deterrent effect. Bacillus thuringiensis var. israelensis (Bti) satisfies the latter criterion, but lures for these autocidal gravid traps are sorely needed. We observed that gravid Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus laid significantly more eggs in cups with extracts from 4th-stage larvae (4 L) of the same or different species. No activity was found when 4 L were extracted with hexane, diethyl ether, methanol, or butanol, but activity was observed with dimethyl sulfoxide extracts. Larval extracts contained both oviposition stimulant(s)/attractant(s) and deterrent(s), which partitioned in the water and hexane phases, respectively. Lyophilized larval extracts were active after a month, but activity was reduced by keeping the sample at 4 °C. In the tested range of 0.1 to 1 larvae-equivalent per milliliter, oviposition activity increased in a dose-dependent manner. In field experiments, Ae. aegpti laid significantly more eggs in traps loaded with larval extracts plus Bti than in control traps with water plus Bti.

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Section: Resultsmentioning

confidence: 97%

“…Inspired by preliminary and promising experiments with Ae. albopictus 10 , we tested extracts at 1 larva-equivalent or 1 pupa-equivalent per 3 ml of water, or 0.33 equivalent per ml. First, we obtained aqueous extracts from L4 larvae of Ae.…”

Section: Resultsmentioning

confidence: 99%

Conspecific and allospecific larval extracts entice mosquitoes to lay eggs and may be used in attract-and-kill control strategy

Faierstein

Sena

et al. 2019

Sci Rep

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“…In this study, gravid Ae. aegypti were preferentially attracted to oviposit in response to the VOCs emanating from water conditioned with late-stage larvae [ 11 , 20 , 22 ], which likely signals a productive breeding site and reduced competition for resources between the existing, soon to pupate, larvae and the new generation [ 6 , 20 , 41 , 42 ]. Furthermore, the density of the conspecific aquatic stages affected oviposition site choice, with gravid mosquitoes laying fewer eggs, and even avoiding ovipositing, on the treated sites when the odours of these sites indicated high densities of conspecific competitors [this study, 12 , 15 , 17 , 18 , 24 , 25 , 41 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…This is, therefore, dependent on the ability of gravid females to evaluate cues emanating from distinct conspecific aquatic stages, as these provide reliable signals of breeding site conditions [ 11 , 12 , 15 , 18 , 19 ]. Limitation in nutrient resources, regulated by, e.g., competition and the dynamic nature of mosquito-associated microbial communities, differentially affects oviposition site choice in a taxon-dependent manner [ 5 , 6 , 19 , 21 , 22 ]. In addition, the persistence of individual breeding sites affects mosquitoes species-specifically, often depending on the drought tolerance of the aquatic stages, with conspecific stage-associated cues providing reliable temporal information concerning, e.g., ephemeral and cyclically flooded sites [ 2 , 6 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the persistence of individual breeding sites affects mosquitoes species-specifically, often depending on the drought tolerance of the aquatic stages, with conspecific stage-associated cues providing reliable temporal information concerning, e.g., ephemeral and cyclically flooded sites [ 2 , 6 , 21 ]. For example, gravid yellow fever and Asian tiger mosquitoes Aedes aegypti and Aedes albopictus , respectively, preferentially select breeding sites that contain or have previously contained, late aquatic stages, which is believed to indicate the availability of larval food resources [ 20 , 22 ]. The density of the conspecific aquatic stages in the breeding sites also modulates mosquito oviposition site selection, as overcrowding leads to competition while low conspecific densities increase the risk of predation, with optimal densities being species-dependent [ 12 , 15 , 17 , 18 , 23 – 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Odour-mediated oviposition site selection in Aedes aegypti depends on aquatic stage and density

2023

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Background Olfaction plays an important role in the selection and assessment of oviposition sites by mosquitoes. Volatile organic compounds (VOCs) associated with potential breeding sites affect the behaviour of gravid mosquitoes, with VOCs from aquatic stages of conspecific mosquitoes influencing and regulating oviposition. The purpose of this study was to conduct a systematic analysis of the behavioural response of gravid Aedes aegypti to conspecific aquatic stage-conditioned water, to identify the associated bioactive VOCs and to determine how blends of these VOCs regulate oviposition site selection and stimulate egg-laying. Methods Using a multi-choice olfactory oviposition assay, controlling for other sensory modalities, the responses of individual females to water conditioned with different densities of conspecific aquatic stages were assessed. The conditioned water samples from the most preferred density of each aquatic stage were subsequently compared to each other using the same oviposition assay and analysed using an analysis of variance (ANOVA) followed by a Tukey post-hoc test. Using combined gas chromatography and electroantennographic detection or mass spectrometry, bioactive VOCs from the preferred density of each aquatic stage were identified. Synthetic blends were prepared based on the identified ratios of bioactive VOCs in the aquatic stages, and then tested to determine the oviposition choice of Ae. aegypti in a dose-dependent manner, against a solvent control, using a dual-choice assay. This dataset was analysed using nominal logistic regression followed by an odds ratio comparison. Results Gravid Ae. aegypti responded stage- and density-dependently to water conditioned with eggs, second- and fourth-instar larvae, and pupal exuviae, but not to water conditioned with pupae alone. Multi-choice assays demonstrated that gravid mosquitoes preferred to oviposit in water conditioned with fourth-instar larvae, over the other aquatic stage-conditioned water. Gravid Ae. aegypti were attracted, and generally stimulated, to oviposit in a dose-dependent manner to the individual identified synthetic odour blends for the different aquatic stages. Conclusions Intraspecific VOCs regulate oviposition site selection in Ae. aegypti in a stage- and density-dependent manner. We discuss the need for further studies to evaluate the identified synthetic blends to modulate the odour-mediated oviposition of Ae. aegypti under field conditions. Graphical Abstract

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Reservatórios domiciliares de água e controle do Aedes aegypti

Forattini

Brito

2003

Rev. Saúde Pública

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Os reservatórios domiciliares de água, comumente conhecidos como caixas d'água, constituem fonte de desenvolvimento do Aedes aegypti. Em áreas com edificações precárias existe tendência para situar essas caixas sobre a laje das casas. Todavia, observa-se na arquitetura moderna a mesma situação em relação a esses recipientes, mesmo em condomínios de luxo. Assim sendo, chama-se a atenção para a necessidade de, na vigilância entomológica, ter cuidado especial para tais reservatórios domiciliares de água.

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Influência de extratos de formas evolutivas sobre atividades de oviposição de fêmeas de Aedes (s) albopictus (Skuse)

Cited by 6 publications

References 6 publications

Conspecific and allospecific larval extracts entice mosquitoes to lay eggs and may be used in attract-and-kill control strategy

Conspecific and allospecific larval extracts entice mosquitoes to lay eggs and may be used in attract-and-kill control strategy

Odour-mediated oviposition site selection in Aedes aegypti depends on aquatic stage and density

Reservatórios domiciliares de água e controle do Aedes aegypti

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