“…Studies on physid species in the Neotropics and particularly in Brazil, are mostly concerned to the invasive P. acuta, and the native S. marmorata (Coimbra-Junior & Santos 1986, Paraense 1986, Teles et al 1991, Vaz et al 1992, Souza et al 1998, Thiengo et al 1998, 2001, 2002a, 2002b, 2005, Carvalho et al 2001, Fernandez et al 2010, Maldonado et al 2001, Medeiros et al 2002, Queiroz et al 2002, Callisto et al 2005 Consequently, the operational criteria used to delimit these species remain largely based on shell morphology, impairing the estimation of species diversity and distribution, as the shells of South American physids, with few exceptions, are very similar (Fig. 4).…”