Estudo sobre a utilização de Rhodnius neglectus para xenodiagnósticos realizados em marsupiais (Didelphis)

Forattini, Oswaldo Paulo; Cotrim, Maria das Dores; Galati, Eunice Aparecida Bianchi; Sarzana, Silvia Branco; Cruz, Cristina Ferez; Dinteren, Nadja Helena Sabbag Van; Gotlieb, Sabina Léa Davidson

doi:10.1590/s0034-89101976000400007

Cited by 4 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The captured sylvatic animals were anaesthetised using 2.5% sodium thiopental and submitted to xenodiagnoses. Ten 3rd or 4th-stage Rhodnius neglectus nymphs were used for each animal ( Forattini et al 1976 ). A sample of each species of captured animal was culled (authorised by the Brazilian Institute of Environment and Renewable Natural Resources IBAMA - license number 11019-1, registration number 1908597).…”

Section: Methodsmentioning

confidence: 99%

Chagas disease ecoepidemiology and environmental changes in northern Minas Gerais state, Brazil

Vianna

Guimarães

Souza³

et al. 2017

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

BACKGROUND Triatoma sordida and Triatoma pseudomaculata are frequently captured triatomine species in the Brazilian savannah and caatinga biomes, respectively, and in Brazilian domiciles.OBJECTIVESThis study identified eco-epidemiological changes in Chagas disease in northern Minas Gerais state, Brazil, and considered the influence of environmental shifts and both natural and anthropogenic effects.METHODSDomicile infestation and Trypanosoma cruzi infection rates were obtained from triatomines and sylvatic reservoirs during the following two time periods: the 1980s and 2007/2008. Entomological and climatic data with land cover classification derived from satellite imagery were integrated into a geographic information system (GIS), which was applied for atmospheric correction, segmentation, image classification, and mapping and to analyse data obtained in the field. Climatic data were analysed and compared to land cover classifications.RESULTSA comparison of current data with data obtained in the 1980's showed that T. sordida colonised domiciliary areas in both periods, and that T. pseudomaculata did not colonise these areas. There was a tendency toward a reduction in T. cruzi infection rates in sylvatic reservoirs, and of triatomines captured in both households and in the sylvatic environment. T. sordida populations have reduced in the sylvatic environment, while T. pseudomaculata showed an expanding trend in the region compared to counts observed in the 1980's in the sylvatic environment. This may be related to high deforestation rates as well as gradual increases in land surface temperature (LST) and temperatures along the years.MAIN CONCLUSIONSOur results suggest a geographical expansion of species into new biomes as a result of anthropogenic and climatic changes that directly interfere with the reproductive and infection processes of vectors.

show abstract

Section: Methodsmentioning

confidence: 99%

Chagas disease ecoepidemiology and environmental changes in northern Minas Gerais state, Brazil

Vianna

Guimarães

Souza³

et al. 2017

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…In microscopy of the rectal contents obtained by abdominal compression of 2,016 larvae and adults of T. infestans from the field an infection rate of 46.7% was found; further investigation of 625 previously negative individuals showed an infection in an additional 100 bugs (Guedes 1952). In another analysis of the rectal contents obtained by abdominal compression 3.5 days after infection, one out of seven groups of R. neglectus was classified as positive, but after dissection of the posterior intestine six out of seven groups were positive (Forattini et al 1976). Examination of pooled rectal contents of third instars of T. infestans instead of an individual examination of rectal contents required two or three times more bugs to obtain similar or superior results .…”

Section: Disadvantages Of Xenodiagnosis Of Chagas Disease: Sensitivitymentioning

confidence: 99%

“…If the rectum was well colonized about 3 months after infection and monthly feeding, starvation reduced the population density, but even after starvation periods of 4 or 11 months some parasites remained attached to the rectal cuticle (Schaub and L€ osch 1989;Kollien and Schaub 1998b). One week and 17 days after xenodiagnosis, the rectal content of five and six groups out of seven groups of Rhodnius neglectus, respectively, contained trypanosomes, but the highest infection rates were detectable 3 weeks after xenodiagnosis (Forattini et al 1976). Also in seven out of 16 groups of P. megistus, T. infestans, R. neglectus, and R. prolixus fed on guinea pigs in the chronic phase, the percentages of positive bugs increased between 15 and 30 days after feeding (Perlowagora-Szumlewicz and Muller 1987).…”

Section: Disadvantages Of Xenodiagnosis Of Chagas Disease: Time Involmentioning

confidence: 99%

“…A comparison of three of the major domestic vectors of Chagas disease -R. prolixus, T. infestans, and P. megistus -using a monkey in a stage of infection comparable to the human chronic stage and a Peruvian strain of T. cruzi resulted in infection rates of 56.8% for R. prolixus and about 75% for the two other species (Miles et al 1975). However, another Rhodnius species, the sylvatic R. neglectus, was more susceptible than T. infestans after feeding on a naturally infected opossum (Forattini et al 1976). Using a fresh T. cruzi isolate originating from Triatoma vitticeps and acute phase mice, all R. neglectus but only 39% of T. infestans and 83% of P. megistus became infected (Kollien et al 1998).…”

Section: Disadvantages Of Xenodiagnosis Of Chagas Disease: Sensitivitymentioning

confidence: 99%

See 1 more Smart Citation

Xenodiagnosis

Meiser¹,

Schaub²

2011

Nature Helps...

View full text Add to dashboard Cite

In xenodiagnosis, the vector of a pathogenic agent is infected to make identification of the parasite easier after a period of multiplications. This enables a diagnosis if the strain of the pathogen possesses a low virulence or if very low numbers of parasites occur in the blood, for example Trypanosoma cruzi in the chronic phase of Chagas disease. In 1914, 5 years after the discovery of T. cruzi in the triatomines, its blood-sucking vectors, these were used for the first time as a diagnostic tool of this disease. For many decades thereafter xenodiagnosis represented the golden standard. Meanwhile serological and molecular biological methods are preferably used for diagnosis today, but xenodiagnosis and in vitro cultivation are still the best methods for a proof of the living parasites or for the isolation of the flagellate. In this context, the different methodological variations and the disadvantages and advantages of xenodiagnosis are discussed in our review.

show abstract

Susceptibilidade de Rhodnius neglectus, Rhodnius robustus e Triatoma infestans (Hemiptera, Reduviidae, Triatominae) à infecção por duas cepas de Trypanosoma cruzi (Kinetoplastidae, Trypanosomatidae) utilizando xenodiagnóstico artificial

Martins

Rosa

Castanho

et al. 2000

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Resumo A susceptibilidade de ninfas de 3º estádio de Rhodnius neglectus, R. robustus e Triatoma infestans às cepas Y e AMJM de Trypanosoma cruzi foi verificada utilizando xenodiagnóstico artificial. Para a leitura do xenodiagnóstico, as fezes dos triatomíneos foram examinadas a cada dois dias, a partir do 5º até o 31º dia pós infecção, pela técnica de compressão abdominal. Os resultados mostraram diferenças na susceptibilidade dos triatomíneos para as duas cepas estudadas e o período ótimo de leitura variou do 11º ao 19º dias para a cepa Y e do 11º ao 15º dias para a cepa AMJM. Também, pôde-se concluir que para a cepa Y, as três espécies de triatomíneos demonstraram boa susceptibilidade, enquanto para a cepa AMJM, a melhor susceptibilidade foi observada com R. neglectus, seguida pelo T. infestans e R. robustus. Palavras-chaves: Rhodnius neglectus. Rhodnius robustus. Triatoma infestans. Trypanosoma cruzi. Xenodiagnóstico artificial.Abstract The susceptibility of 3 rd instar nymph of Triatominae Rhodnius neglectus, R. robustus and Triatoma infestans to Trypanosoma cruzi Y and AMJM strains was verified using artificial xenodiagnosis. After the accomplishment of the xenodiagnosis, the faeces of the Triatominae were analyzed on two-day intervals from day 5 until day 31 post infection, using the abdominal compression technique. The results showed differences in the susceptibility of the Triatominae for the two strains studied, and the optimal period reading differed from day 11 to day 19 for the Y strain and from day 11 to day 15 for the AMJM strain. For the Y strain, all three Triatominae species showed good susceptibility, whereas in the AMJM strain, the highest susceptibility was observed with R. neglectus, followed by T. infestans and R. robustus.

show abstract

Estudo sobre a utilização de Rhodnius neglectus para xenodiagnósticos realizados em marsupiais (Didelphis)

Cited by 4 publications

References 4 publications

Chagas disease ecoepidemiology and environmental changes in northern Minas Gerais state, Brazil

Chagas disease ecoepidemiology and environmental changes in northern Minas Gerais state, Brazil

Xenodiagnosis

Susceptibilidade de Rhodnius neglectus, Rhodnius robustus e Triatoma infestans (Hemiptera, Reduviidae, Triatominae) à infecção por duas cepas de Trypanosoma cruzi (Kinetoplastidae, Trypanosomatidae) utilizando xenodiagnóstico artificial

Contact Info

Product

Resources

About