Estudo sorológico de infecções ocasionadas por citomegalovírus

Candeias, J. A. N.; Stewien, Klaus Eberhard; Barbosa, Victório

doi:10.1590/s0034-89101974000300002

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…It also allows the molecular and epidemiological profiling of virus isolates from individuals with risk factors for infection, such as immunosuppression and living in an underdeveloped country where the HCMV seroprevalence is elevated [9][10][11][12][13] . Molecular analyses by restriction fragment length polymorphism (RFLP) and sequencing of the glycoprotein B (gB)-encoding region of the HCMV viral envelop are used as basic tools for genotypic classification of the virus and the association between genotypes and immunodeficient patient categories as assessed by clinicians [14][15][16][17][18][19][20][21][22][23][24] . Rasmussen associated this genotypic variability with graft rejection in liver transplant patients and with the level of neutropenia in organ recipients, which can result in death.…”

Section: Introductionmentioning

confidence: 99%

Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil

Silva

Medeiros

Moraes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction:Human cytomegalovirus is an opportunistic betaherpesvirus that causes persistent and serious infections in immunodeficient patients. Recurrent infections occur due to the presence of the virus in a latent state in some cell types. It is possible to examine the virus using molecular methods to aid in the immunological diagnosis and to generate a molecular viral profile in immunodeficient patients. The objective of this study was to characterize cytomegalovirus genotypes and to generate the epidemiological and molecular viral profile in immunodeficient patients. Methods: A total of 105 samples were collected from immunodeficient patients from the City of Belém, including newborns, hemodialysis patients, transplant recipients and HIV+ patients. An IgG and IgM antibody study was completed using ELISA, and enzymatic analysis by restriction fragment length polymorphism (RFLP) was performed to characterize viral genotypes. Results: It was observed that 100% of the patients had IgG antibodies, 87% of which were IgG+/IgM-, consistent with a prior infection profile, 13% were IgG+/IgM+, suggestive of recent infection. The newborn group had the highest frequency (27%) of the IgG+/IgM+ profile. By RFLP analysis, only one genotype was observed, gB2, which corresponded to the standard AD169 strain. Conclusions: The presence of IgM antibodies in newborns indicates that HCMV continues to be an important cause of congenital infection. The low observed genotypic diversity could be attributed to the small sample size because newborns were excluded from the RFLP analysis. This study will be continued including samples from newborns to extend the knowledge of the general and molecular epidemiology of HCMV in immunodeficient patients.

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Section: Introductionmentioning

confidence: 99%

Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil

Silva

Medeiros

Moraes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Prevalence of Cytomegalovirus Antibodies in Brazilian and Japanese Populations in the North‐East of Brazil

Linhares

Andrade

Tateno

et al. 1989

Microbiology and Immunology

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The serological status to cytomegalovirus (CMV) was examined for 616 Brazilians and 399 Japanese immigrants living in the North-East of Brazil. The sera were screened for IgG antibodies to CMV by enzyme-linked immunosorbent assay (ELISA). The overall prevalence of CMV antibodies was higher in the Japanese population (83.7%) than in the Brazilian population (69.8%). The seropositivity was analyzed by factors of age, sex, ethnic background, and socioeconomic status. Mother-baby contact seems to be a factor of significance in the seroepidemiology of CMV infection.Human cytomegalovirus (CMV) is a ubiquitous herpesvirus which exclusively infects humans. CMV can spread vertically as well as horizontally. Because CMV is unstable in the environment, close or intimate contact is required for its spread. Sources of CMV include saliva, urine, tears, breast milk, cervical excretions, semen, and blood. Thus, seroepidemiological features of CMV infection reflect the hygienic practice or the way of life of the population. Generally, socioeconomic status is a major determinant of the prevalence of CMV infection. In fact, the age at which primary CMV infection occurs is much younger in developing countries than in developed countries (12, 13). Race, sex, sexual activity, and blood group are also suggested to be factors of significance in the prevalence of CMV infection (6, 7,15,16,18,19).CMV infection is usually asymptomatic in normal hosts. However, CMV may produce severe diseases in fetuses and immunocompromised hosts (2,9,14,20). Since symptomatic CMV infections mostly occur in seronegative individuals, serological status of a population is of great importance. In Brazil, a serological survey revealed that 47% of Brazilians of 0 to 60 years of age in Sao Paulo in the South of Brazil were positive for CMV antibodies (3). However, this survey was done by the relatively insensitive complement fixation (CF) test. In the present study, we determined the serological status to CMV of healthy individuals in the North-East of Brazil by sensitive enzyme-linked immunosorbent assay (ELISA). Subjects included Brazilians and Japanese immigrants living in Recife and other

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Detección de citomegalovirus en plasma de pacientes con VIH mediante PCR dúplex en tiempo real

Valdez-Salazar

Sánchez-Nájera

Ribas‐Aparicio

et al. 2021

RMIMSS

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Estudo sorológico de infecções ocasionadas por citomegalovírus

Cited by 3 publications

References 22 publications

Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil

Restriction enzyme analysis of the human cytomegalovirus genome in specimens collected from immunodeficient patients in Belém, State of Pará, Brazil

Prevalence of Cytomegalovirus Antibodies in Brazilian and Japanese Populations in the North‐East of Brazil

Detección de citomegalovirus en plasma de pacientes con VIH mediante PCR dúplex en tiempo real

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