New data on the natural history and description of the immatures of Fulgeochlizus bruchi, a bioluminescent beetle from Central Brazil (Elateridae, Pyrophorini)

Rosa, Simone Policena; Costa, Cleide; Higashi, Neide

doi:10.1590/s0031-10492010004100001

Cited by 8 publications

(6 citation statements)

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“…As previously shown, 30 the F. bruchi click-beetle is unique by displaying only a functional abdominal lantern (Fig. 1), which emits green bioluminescence.…”

Section: Bioluminescence Anatomical Location and Functionsupporting

confidence: 55%

“…During the spring evenings, adult males fly emitting an intense greenish light from the abdominal lantern, whereas females in the grass answer with their abdominal lanterns. 30 Here we report the cloning, sequence analysis and characterization of the luciferase cDNA from the abdominal lantern of this click-beetle and the expression and characterization of the recombinant enzyme.…”

Section: Introductionmentioning

confidence: 99%

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Luciferase from Fulgeochlizus bruchi (Coleoptera:Elateridae), a Brazilian click-beetle with a single abdominal lantern: molecular evolution, biological function and comparison with other click-beetle luciferases

Amaral

Prado

Viviani

2012

Photochem Photobiol Sci

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Bioluminescent click-beetles emit a wide range of bioluminescence colors (λ(Max) = 534-594 nm) from thoracic and abdominal lanterns, which are used for courtship. Only the luciferases from Pyrophorus and Pyrearinus species were cloned and sequenced. The Brazilian Fulgeochlizus bruchi click-beetle, which inhabits the Central-west Cerrado (Savannas), is noteworthy because, differently from other click-beetles, the adult stage displays only a functional abdominal lantern, which produces a bright green bioluminescence for sexual attraction purposes, and lacks functional thoracic lanterns. We cloned the cDNA for the abdominal lantern luciferase of this species. Notably, the primary sequence of this luciferase showed slightly higher identity with the green emitting dorsal lantern luciferases of the Pyrophorus genus instead of the abdominal lanterns luciferases. This luciferase displays a blue-shifted spectrum (λ(Max) = 540 nm), which is pH-insensitive from pH 7.5 to 9.5 and undergoes a slight red shift and broadening above this pH; the lowest K(M) for luciferin among studied click-beetle luciferases, and the highest optimum pH (9.0) ever reported for a beetle luciferase. At pH 9.0, the K(M) for luciferin increases, showing a decrease of affinity for this substrate, despite the higher activity. The slow luminescence decay rate of F. bruchi luciferase in vitro reaction could be an adaptation of this luciferase for the long and sustained in vivo luminescence display of the click-beetle during the courtship, and could be useful for in vivo intracellular imaging.

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“…As previously shown, 30 the F. bruchi click-beetle is unique by displaying only a functional abdominal lantern (Fig. 1), which emits green bioluminescence.…”

Section: Bioluminescence Anatomical Location and Functionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Luciferase from Fulgeochlizus bruchi (Coleoptera:Elateridae), a Brazilian click-beetle with a single abdominal lantern: molecular evolution, biological function and comparison with other click-beetle luciferases

Amaral

Prado

Viviani

2012

Photochem Photobiol Sci

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“…Our analyses also showed a closer relationship between the species Pyrophorus divergens and Fulgeochlizus bruchi . A similar result was also shown by luciferases sequences analysis, with high support value , and by morphologic studies . However, only a few genera were used in this analysis, and other taxa are necessary for more detailed analysis.…”

Section: Resultssupporting

confidence: 82%

Molecular phylogeny of Neotropical bioluminescent beetles (Coleoptera: Elateroidea) in southern and central Brazil

et al. 2013

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Bioluminescence in beetles is found mainly in the Elateroidea superfamily (Elateridae, Lampyridae and Phengodidae). The Neotropical region accounts for the richest diversity of bioluminescent species in the world with about 500 described species, most occurring in the Amazon, Atlantic rainforest and Cerrado (savanna) ecosystems in Brazil. The origin and evolution of bioluminescence, as well as the taxonomic status of several Neotropical taxa in these families remains unclear. In order to contribute to a better understanding of the phylogeny and evolution of bioluminescent Elateroidea we sequenced and analyzed sequences of mitochondrial NADH2 and the nuclear 28S genes and of the cloned luciferase sequences of Brazilian species belonging to the following genera: (Lampyridae) Macrolampis, Photuris, Amydetes, Bicellonycha, Aspisoma, Lucidota, Cratomorphus; (Elateridae) Conoderus, Pyrophorus, Hapsodrilus, Pyrearinus, Fulgeochlizus; and (Phengodidae) Pseudophengodes, Phrixothrix, Euryopa and Brasilocerus. Our study supports a closer phylogenetic relationship between Elateridae and Phengodidae as other molecular studies, in contrast with previous morphologic and molecular studies that clustered Lampyridae/Phengodidae. Molecular data also supported division of the Phengodinae subfamily into the tribes Phengodini and Mastinocerini. The position of the genus Amydetes supports the status of the Amydetinae as a subfamily. The genus Euryopa is included in the Mastinocerini tribe within the Phengodinae/Phengodidae.

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“…The larval bioluminescent pattern of Alampoides alychnus (Kirsh) differs from the luminescent patterns presented by the Pyrophorini species (Costa, 1970;Casari-Chen & Costa, 1986, Rosa et al 2010, especially in the absence of luminescent spots on the prothorax (Fig. 2A).…”

Section: Discussionmentioning

confidence: 79%

Description of the larva of Alampoides alychnus (Kirsch, 1873), the first known species with bioluminescent immatures in Euplinthini (Elateridae, Agrypninae)

Rosa¹,

Costa²

2013

Pap. Avulsos Zool. (São Paulo)

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New data on the natural history and description of the immatures of Fulgeochlizus bruchi, a bioluminescent beetle from Central Brazil (Elateridae, Pyrophorini)

Cited by 8 publications

References 0 publications

Luciferase from Fulgeochlizus bruchi (Coleoptera:Elateridae), a Brazilian click-beetle with a single abdominal lantern: molecular evolution, biological function and comparison with other click-beetle luciferases

Luciferase from Fulgeochlizus bruchi (Coleoptera:Elateridae), a Brazilian click-beetle with a single abdominal lantern: molecular evolution, biological function and comparison with other click-beetle luciferases

Molecular phylogeny of Neotropical bioluminescent beetles (Coleoptera: Elateroidea) in southern and central Brazil

Description of the larva of Alampoides alychnus (Kirsch, 1873), the first known species with bioluminescent immatures in Euplinthini (Elateridae, Agrypninae)

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