Abstract.—During a recent expedition to five protected areas in Panama (Parque Nacional Volcán Barú andReserva Forestal Fortuna, Chiriquí Province; Parque Nacional Santa Fe, Veraguas Province; Parque Nacional Darien,Rancho Frío, Darien Province; and Mamoni Valley Private Reserve, Panama Province), we observed 95 species ofamphibians and reptiles (51 amphibians and 44 reptiles). We made opportunistic observations of many of them,including noteworthy updates on the conservation status of critically endangered or endangered taxa (Atelopus glyphus,A. limosus, A. varius, Isthmohyla angustilineata, I. tica, Oedipina grandis), the third and fourth distribution records forPanama of Trilepida macrolepis, the second distribution report of Leptophis cupreus for the country, the fourth countryrecord of Bachia blairi, new behavior and maximum size for Oophaga vicentei, and the second known specimen ofNorops triumphalis.