Análise sensorial do leite humano ordenhado e sua carga microbiana

Abstract

Objective: To verify the existence of a relationship between presence of off-flavor and microorganism load in quality control rejected samples of expressed human milk from a donor milk bank. Methods:A total of 30 samples of expressed human milk with off-flavor were tested for the occurrence of the following microorganisms: aerobic mesophilic, psycrotrophic, proteolytic, psycrotrophic proteolytic, thermoduric, psycrotrophic thermoduric, lactate and lipolytic bacteria, molds and yeasts and Staphylococcus aureus, total coliforms and thermophilic coliforms, in accordance with official methods.Results: Percentage occurrence of microorganisms was as follows: aerobic mesophilic = 80%; psycrotrophic = 36.7%; proteolytic = 46.7%; psycrotrophic proteolytic = 16.7%; thermoduric = 6.7%; psycrotrophic thermoduric = 0%; lactate bacteria = 50%; lipolytic = 10%; molds and yeasts = 6.7%; S. aureus = 30%; total coliforms = 53.3%; and thermophilic coliforms = 16.7%. Conclusion:A consistent relationship between presence of off-flavor and elevated microorganism counting was observed in the analyzed samples. This correlation highlights the importance of off-flavor research during selection and quality control processes in human milk banks.J Pediatr (Rio J). 2008;84(2):181-184: Human milk, milk banks, quality control, food microbiology.

Hygienic-sanitary quality and effect of freezing time and temperature on total antioxidant capacity of human milk

“…The evaluated milks of four mothers had thermotolerant coliform counts <0.3 MPN/mL; however, the milk of the mother M3 presented contamination of 1 MPN/mL. The results of the present study differ from those reported by Novak et al (2008), whose total coliform counts ranged from 10 4 to 10 6 CFU/ mL, and thermotolerant coliforms in the order of 10 3 and 10 4 CFU/ mL, indicating worse hygienic-sanitary conditions.…”

“…According Serra et al (2013), yeast and fungi growth would indicate inadequate hygienic and sanitary conditions, especially due to the incorrect hand washing. These values are close to those found in the study by Novak et al (2008), who evaluated the microbiological quality of 30 raw HM samples obtained from HMB donors of the Fernandes Figueira Institute. Their counts were around 10 3 CFU/ mL, which may indicate possible flaws in the mothers or utensils hygiene used for extraction.…”

“…Despite having antimicrobial properties that delay the decomposition caused by the primary microbiota, the HM has little efficacy against secondary contaminants from the environment, utensils and mothers (Grazziotin et al, 2010). Thus, it must be handled under hygienic-sanitary conditions suitable to avoid contamination by pathogens (Novak et al, 2008).…”

Impact of perinatal different intrauterine environments on child growth and development in the first six months of life - IVAPSA birth cohort: rationale, design, and methods

“…The women will be advised to wash their hands with soap and water before milking and washing their breasts with water [96,97]. The collection procedure will be performed by the mothers, under appropriate supervision provided by the researcher.…”