Capacidade de germinação e quebra de dormência em sementes de cultivares de videira

Pommer, Celso Valdevino; Maeda, Jocely Andreuccetti; Ribeiro, Ivan José Antunes

doi:10.1590/s0006-87051988000200001

Cited by 2 publications

(8 citation statements)

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“…Segundo Ellis et al (1983), métodos atualmente empregados para germinação de sementes de videiras raramente proporcionam porcentagens superiores a 50%. Pommer et al (1988) obtiveram no máximo 53,2% de germinação de sementes de videira niágara rosada, combinando estratificação por 60 dias e 2.000 mg L -1 de GA 3 . Segundo os mesmos autores, existe tendência natural Figura 2.…”

Section: Resultsunclassified

“…No presente trabalho, a porcentagem máxima de germinação foi de 77%, considerada alta se comparada aos resultados obtidos por outros autores (Scott & Ink, 1950;Ottenwaelter et al,1974;Pommer et al, 1988;Ergenoglu et al, 1997). Esta porcentagem de germinação foi alcançada, basicamente, pela combinação dos tratamentos com 4000 mg L -1 de GA 3 , corte da semente na região da micrópila e ambiente de germinação in vitro .…”

Section: Resultsunclassified

“…cardinal, tarsus bevari e itália durante dois anos, obtiveram no tratamento controle a percentagem de germinação média de 5,9, 2,0 e 4,4, respectivamente. Pommer et al (1988), estudando o efeito da estratificação na germinação de sementes de Vitis labruscana cv. niagara rosada, observaram que as sementes do tratamento testemunha não germinaram.…”

Section: Introductionunclassified

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Quebra de dormência de sementes da videira cv. niágara rosada sem estratificação

et al. 2010

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Breaking the dormancy of niagara rosada seeds without stratificationThe objective of this study was to asses the effect of three factors on the germination of grape seeds, cv. Niagara Rosada. The three studied factors were: physical treatments, chemical treatments and germination environment. The physical treatments consisted of whole seeds, seeds with a cross cut in the median region and seeds with a cut in the micropyle. The chemical treatments were applied by immersing whole seeds in aqueous solution of gibberellic acid (GA 3 ) with concentrations of 0 and 4000 mg L -1 . The environments evaluated were in vitro and ex vitro. The experiment was arranged in a factorial 3 x 2x 2, randomized blocks design, with 4 repetitions, each one consisting of a Gerbox® containing 25 seeds or a rack with 25 test tubes. The parameters evaluated were the percentage and speed of seed germination. The results showed that cutting is essential to the germination of non-stratified seeds. The combination micropyle cut, 4000 mg L -1 of GA 3 and in vitro environment resulted in 77% of seed germination. The in vitro environment was significantly superior to the ex vitro environment.

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Section: Resultsunclassified

Section: Introductionunclassified

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Quebra de dormência de sementes da videira cv. niágara rosada sem estratificação

et al. 2010

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“…The seed propagation is commonly adopted in breeding programs to obtain new hybrids (Ritschel et al, 2014;Santos et al, 2019). However, grape seeds have dormancy resulting in low germination rates which hinders and delays grapevines breeding programs (Conner, 2008;Ellis;Hong;Roberts, 1983;Generoso et al, 2019;Pereira;Terra, 1985;Pommer;Ribeiro, 1988;Val;Alvarenga;Cecon, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…To break dormancy in grape seeds, the Rules for Seed Testing (RST) (Brasil, 2009) recommend stratification in moist substrate at temperatures between 3 and 5 °C for 90 days, before germination tests. However, this strategy requires a significant period of time and results in germination that hardly exceed 50% (Conner, 2008;Ellis;Hong;Roberts, 1983;Pommer;Ribeiro, 1988).…”

Section: Introductionmentioning

confidence: 99%

Overcoming germination barriers through in vitro culture of mature zygotic embryos of grapevine cultivars

Júnior

Carvalho

Generoso

et al. 2022

PCC&M

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Grape seeds have physical and physiological dormancy. Conventional germination methods are not very efficient. The in vitro culture of seeds and of mature zygotic embryos can be an alternative for overcoming germination barriers. The objective was to develop methodologies that increase seed germination and reduce the time to obtain seedlings. The following assays were developed: (1) conventional ex vitro germination, in a 2 × 3 factorial arrangement, of seeds with or without stratification for 90 days, from cultivars 'Niágara Rosada', 'Itália' and 'Red Globe'; (2) in vitro seed germination, in a 3 × 3 factorial arrangement, of the three cultivars and three explants (intact seeds, seeds sectioned in the micropyle region and seeds with a cross-section); and (3) in vitro germination of mature zygotic embryos, in a 4 × 2 factorial arrangement represented by four concentrations of mineral salts (MS, ½MS, WPM and ½WPM) and two cultivars ('Niágara Rosada' and 'Itália'). The conventional ex vitro germination assay resulted in a low seed germination for the three cultivars. The two types of section associated with in vitro culture provided an increase in seed germination. However, the highest germination rate (over 90%) in the shortest time (17 days) was achieved by the embryos grown in vitro. This study defines an efficient methodology to obtain high germination in grape seeds-over 85%-with uniformity and in a short time, using mature zygotic embryos grown in ½WPM culture medium. This methodology can accelerate grapevine breeding programs.

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Capacidade de germinação e quebra de dormência em sementes de cultivares de videira

Cited by 2 publications

References 1 publication

Quebra de dormência de sementes da videira cv. niágara rosada sem estratificação

Quebra de dormência de sementes da videira cv. niágara rosada sem estratificação

Overcoming germination barriers through in vitro culture of mature zygotic embryos of grapevine cultivars

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