Especificidade hospedeira e pré-seleção de estirpes de Rhlzobium japonicum para as variedades Santa-Rosa, viçoja e IAC-2 de soja

Lopes, Eli Sidney; Giardini, A.; Kiihl, Romeu A. S.; Igue, Toshio

doi:10.1590/s0006-87051976000100001

Cited by 7 publications

(5 citation statements)

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“…Brazilian soils are usually very poor in N and are originally free of soybean bradyrhizobia [8,13,15,16,29,30]. Therefore, with the introduction of the soybean crop to the country, inoculants containing foreign strains were used.…”

Section: Discussionmentioning

confidence: 99%

“…As Brazilian soils are originally free from soybean bradyrhizobia [8,13,15,16,29,30], during the establishment of the crop, the seeds had to be inoculated with strains. The inoculant strains were initially brought from the USA, but a search for strains adapted to Brazilian conditions started concomitantly with the expansion of commercial soybean crop production [6,8,15,16,18,19]. The selection of adapted strains usually consisted of inoculation of an area, followed, after a short period of time, by reisolation of bacteria from nodules of soybean trap plants.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of soybean Bradyrhizobium strains adapted to the Brazilian savannas

Santos¹

1999

FEMS Microbiology Ecology

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Brazilian soils are originally free from soybean bradyrhizobia and the first inoculants were brought to the country in this century, but a search for adapted strains started immediately and still continues. A strain selection program was established at Embrapa based on the reisolation of strains after a long period of adaptation to the soils followed by a search for variant genotypes with higher N 2 fixation capacity and competitiveness. A second approach of this program consists of searching for variant colonies of a single strain with higher N 2 fixation rates and competitiveness, following a short period of adaptation to the soil. In this study, using both approaches, strains belonging to three serogroups, CB 1809, 532C and SEMIA 5020, were obtained. In general, the variant strains showed differences in colony morphology (mucoidy) but produced similar protein and lipopolysaccharide profiles. Within serogroup CB 1809, containing variants obtained via the second approach, a low level of DNA polymorphism was detected relative to the parental genotype by ERIC and REP-PCR. However, within the two other serogroups, containing variant strains obtained via the first approach, a high level of polymorphism in ERIC and REP-PCR fingerprints was observed relative to the putative serologically related parental genotypes. These results show that a great variability can be detected following adaptation of Bradyrhizobium strains to the soil, although other potential explanations for the DNA polymorphisms observed are discussed. Some of the variant strains obtained by both methodologies were found to have higher rates of N 2 fixation and almost all were more competitive than the parental genotypes, suggesting that it is possible to select variant strains which can contribute to an improved plant N nutrition status. ß

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of soybean Bradyrhizobium strains adapted to the Brazilian savannas

Santos¹

1999

FEMS Microbiology Ecology

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show abstract

Section: Discussionmentioning

confidence: 99%

“…Soybean was introduced into Brazil in the last century, but large‐scale commercial crop production started in the early 1960s. As Brazilian soils are originally free from soybean bradyrhizobia [8,13,15,16,29,30], during the establishment of the crop, the seeds had to be inoculated with strains. The inoculant strains were initially brought from the USA, but a search for strains adapted to Brazilian conditions started concomitantly with the expansion of commercial soybean crop production [6,8,15,16,18,19].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of soybean Bradyrhizobium strains adapted to the Brazilian savannas

Santos

Vargas

Hungría

1999

FEMS Microbiology Ecology

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Brazilian soils are originally free from soybean bradyrhizobia and the first inoculants were brought to the country in this century, but a search for adapted strains started immediately and still continues. A strain selection program was established at Embrapa based on the reisolation of strains after a long period of adaptation to the soils followed by a search for variant genotypes with higher N(2) fixation capacity and competitiveness. A second approach of this program consists of searching for variant colonies of a single strain with higher N(2) fixation rates and competitiveness, following a short period of adaptation to the soil. In this study, using both approaches, strains belonging to three serogroups, CB 1809, 532C and SEMIA 5020, were obtained. In general, the variant strains showed differences in colony morphology (mucoidy) but produced similar protein and lipopolysaccharide profiles. Within serogroup CB 1809, containing variants obtained via the second approach, a low level of DNA polymorphism was detected relative to the parental genotype by ERIC and REP-PCR. However, within the two other serogroups, containing variant strains obtained via the first approach, a high level of polymorphism in ERIC and REP-PCR fingerprints was observed relative to the putative serologically related parental genotypes. These results show that a great variability can be detected following adaptation of Bradyrhizobium strains to the soil, although other potential explanations for the DNA polymorphisms observed are discussed. Some of the variant strains obtained by both methodologies were found to have higher rates of N(2) fixation and almost all were more competitive than the parental genotypes, suggesting that it is possible to select variant strains which can contribute to an improved plant N nutrition status.

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Presença e eficiência de Rhizobium Japonicum em solos cultivados ou não com soja, no estado deSão Paulo

Lopes¹,

Giardini²,

Kiihl

1976

Bragantia

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ELI SIDNEY LOPES e ANTÔNIO ROBERTO GIARDINI, Seção de Microbiologia doSolo, e ROMEU A. S. KIIHL ( 2 ), Seção de Leguminosas, Instituto Agronô-mico SINOPSEFoi conduzido um ensaio em solução nutritiva sem nitrogênio, com condições assépticas, usando-se como inoculantes para as plantas amostras de solos LR e LB coletadas em locais já cultivados e não com soja, com o objetivo de verificar a presença de Rhizobium capaz de nodular o cultivar santa-rosa.Verificou-se que solos ainda não cultivados com a leguminosa são desprovidos de Rhizobium japonicum, e também que essa bactéria é encontrada em solos previamente cultivados, fixando nitrogênio em quantidades equivalentes à de uma estirpe selecionada.

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Especificidade hospedeira e pré-seleção de estirpes de Rhlzobium japonicum para as variedades Santa-Rosa, viçoja e IAC-2 de soja

Cited by 7 publications

References 0 publications

Characterization of soybean Bradyrhizobium strains adapted to the Brazilian savannas

Characterization of soybean Bradyrhizobium strains adapted to the Brazilian savannas

Characterization of soybean Bradyrhizobium strains adapted to the Brazilian savannas

Presença e eficiência de Rhizobium Japonicum em solos cultivados ou não com soja, no estado deSão Paulo

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