Partículas esferoidais associadas ao vírus do mosaico das nervuras da mandioca

Kitajima, E. W.; Costa, Á. S.

doi:10.1590/s0006-87051966000100018

Cited by 15 publications

(4 citation statements)

References 14 publications

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“…Infected plants develop chlorosis along the veins which evolves later into a mosaic pattern. The circular double stranded DNA genome of the CVMV is encapsidated in 50-60 nm isometric particles which accumulate in inclusion bodies, in the cytoplasm of infected cells [28]. Based upon this information CVMV was listed as a putative pararetrovirus and a candidate member of the caulimovirus subgroup [23].…”

Section: Introductionmentioning

confidence: 99%

Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter

et al. 1996

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The cassava vein mosaic virus (CVMV) is a double stranded DNA virus which infects cassava plants (Manihot esculenta Crantz) and has been characterized as a plant pararetrovirus belonging to the caulimovirus subgroup. Two DNA fragments, CVP1 of 388 nucleotides from position -368 to +20 and CVP2 of 511 nucleotides from position -443 to +72, were isolated from the viral genome and fused to the uidA reporter gene to test promoter expression. The transcription start site of the viral promoter was determined using RNA isolated from transgenic plants containing the CVMV promoter:uidA fusion gene. Both promoter fragments were able to cause high levels of gene expression in protoplasts isolated from cassava and tobacco cell suspensions. The expression pattern of the CVMV promoters was analyzed in transgenic tobacco and rice plants, and revealed that the GUS staining pattern was similar for each construct and in both plants. The two promoter fragments were active in all plant organs tested and in a variety of cell types, suggesting a near constitutive pattern of expression. In both tobacco and rice plants, GUS activity was highest in vascular elements, in leaf mesophyll cells, and in root tips.

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Section: Introductionmentioning

confidence: 99%

Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter

et al. 1996

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“…Carnation etched ring virus is serologically related to DMV and ClMV (Hollings & Stone, 1969), and both cassava vein mosaic (Kitajima & Costa, 1966) and a virus from Mirabilis nyctaginea (Mich.) Macmill. (Kitajima & Brunt, unpublished) have particles and properties similar to those of DMV and ClMV; the three are probably members of this group.…”

Section: Discussionmentioning

confidence: 99%

Some hosts and properties of dahlia mosaic virus

Brunt¹

1971

Annals of Applied Biology

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S U M M A R YDahlia mosaic virus (DMV) infected twenty-five of the eighty-five plant species from four of eighteen families inoculated, but only dahlias were found naturally infected. DMV infected fourteen members of the Solanaceae, Amaranthaceae and Chenopodiaceae, and eleven of twenty-nine Compositae. Verbesina encelioides was the best plant for diagnosis, assay and source of virus. Systemically infected hosts contained ovoid intracellular inclusions 2.5-10 pm in diameter which were shown by electron microscopy to consist of a finely granular, vacuolated matrix containing numerous virus particles. V. encelioides sap was sometimes infective after dilution to 1/2000 but not 1/3000, after heating for 10 min to 75 "C but not 80 "C, and after 4 days at 18 "C or 32 days at 2 "C. Sap from infected dahlia, Zinnia elegans or Ageratum houstonianum rapidly became non-infective, but extracts made with 0.05 M sodium thioglycollate or 0.03 M sodium diethyldithiocarbamate remained infective for R E S U L T S Detection, field occurrence and identity of the virus Detection.Dahlia leaves contain much phenolic material; this, and the activity of dahlia leaf phenoloxidase, are both increased by virus infection (Martin, 1954;Lawson & Taconis, 1965). Early failures to transmit DMV from infected to healthy dahlias by mechanical inoculation of sap (Brierley, 1933), and transmission rates of 10% or less obtained later (Brierley & Smith, 1950), probably resulted from the rapid inactivation of virus by o-quinones or other complex phenolic materials. Inactivation can be de-

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“…This has isometric particles, c. 50 nm in diameter (Kitajima and Costa, 1966) and the genome consists of ds-DNA, c. 8200 bases long. Initially CVMV was regarded as a tentative member of the caulimovirus group.…”

Section: Cvmd Is Caused By Cassava Vein Mosaic Virus (Cvmv)mentioning

confidence: 99%