Caspase-3 and Bcl-2 expression in glioblastoma: an immunohistochemical study

Tirapelli, Luís Fernando; Bolini, Paulo Henrique Nasser Andre; Tirapelli, Daniela Pretti da Cunha; Peria, Fernanda Maris; Becker, Aline Nazareth Paixão; Saggioro, Fabiano Pinto; Júnior, Carlos Gilberto Carlotti

doi:10.1590/s0004-282x2010000400023

Cited by 35 publications

(34 citation statements)

References 17 publications

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“…In some studies, the percentage of caspase 3+ cells ranges from 7.36 to 59.52% (mean 17.67%) [44], whereas in other cases it ranges from 0.70 to 71.60% (mean 32.60%) [45]. In our study, the immunohistochemical staining and densitometric analysis of electropherograms of Western blot have shown that the expression of the active form of caspase 3 in control and placebo groups were lower than in the AgNPs group.…”

Section: Discussionmentioning

confidence: 99%

The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells

Urbańska¹,

Pająk²,

Orzechowski³

et al. 2016

Nano Online

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Recently, it has been shown that silver nanoparticles (AgNPs) provide a unique approach to the treatment of tumors, especially those of neuroepithelial origin. Thus, the aim of this study was to evaluate the impact of AgNPs on proliferation and activation of the intrinsic apoptotic pathway of glioblastoma multiforme (GBM) cells cultured in an in ovo model. Human GBM cells, line U-87, were placed on chicken embryo chorioallantoic membrane. After 8 days, the tumors were divided into three groups: control (non-treated), treated with colloidal AgNPs (40 μg/ml), and placebo (tumors supplemented with vehicle only). At the end of the experiment, all tumors were isolated.Assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and Western blot analyses. The results show that AgNPs can influence GBM growth. AgNPs inhibit proliferation of GBM cells and seem to have proapoptotic properties. Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced. Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones. Further research focused on the cytotoxic effect of AgNPs on tumor and normal cells should be conducted.

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Section: Discussionmentioning

confidence: 99%

The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells

Urbańska¹,

Pająk²,

Orzechowski³

et al. 2016

Nano Online

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“…The inhibitor of apoptosis XIAP that inhibits its downstream targets CASP3 and CASP7 are also part of the subgraph. XIAP is widely expressed in glioblastoma and might be implicated in radio resistance of glioblastoma (49), while expression of CASP3 is generally low in glioblastoma suggesting a low apoptotic activity in these tumors (50). CASP7 is thought to be relevant for the apoptosis/necrosis balance in glioma, with knockdown of CASP7 resulting in an anti-apoptotic and pro-necrotic response that is often seen in glioblastoma (51).…”

Section: Discussionmentioning

confidence: 99%

An integer linear programming approach for finding deregulated subgraphs in regulatory networks

Backes

Rurainski

Klau

et al. 2011

Nucleic Acids Research

143

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Deregulation of cell signaling pathways plays a crucial role in the development of tumors. The identification of such pathways requires effective analysis tools that facilitate the interpretation of expression differences. Here, we present a novel and highly efficient method for identifying deregulated subnetworks in a regulatory network. Given a score for each node that measures the degree of deregulation of the corresponding gene or protein, the algorithm computes the heaviest connected subnetwork of a specified size reachable from a designated root node. This root node can be interpreted as a molecular key player responsible for the observed deregulation. To demonstrate the potential of our approach, we analyzed three gene expression data sets. In one scenario, we compared expression profiles of non-malignant primary mammary epithelial cells derived from BRCA1 mutation carriers and of epithelial cells without BRCA1 mutation. Our results suggest that oxidative stress plays an important role in epithelial cells of BRCA1 mutation carriers and that the activation of stress proteins may result in avoidance of apoptosis leading to an increased overall survival of cells with genetic alterations. In summary, our approach opens new avenues for the elucidation of pathogenic mechanisms and for the detection of molecular key players.

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“…(continued) Increased (Ray et al 2002;Bodey et al 2004;Tirapelli et al 2010); higher expression of cleaved caspase-3 correlated with longer patient survival (Kobayashi et al 2007); basal caspase-3 activity promoted migration and invasiveness of glioblastoma cells (Gdynia et al 2007). …”

Section: Cathepsin-smentioning

confidence: 99%

Glioma Cell Biology

Šedo¹,

Mentlein²

2014

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Caspase-3 and Bcl-2 expression in glioblastoma: an immunohistochemical study

Cited by 35 publications

References 17 publications

The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells

The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells

An integer linear programming approach for finding deregulated subgraphs in regulatory networks

Glioma Cell Biology

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