Evaluation of white matter in patients with multiple sclerosis through diffusion tensor magnetic resonance imaging

Andrade, Rachel E. Maia de; Gasparetto, Emerson L.; Cruz, Luiz Celso Hygino da; Ferreira, Fabiana Brito; Domingues, R. C.; Marchiori, Edson; Domingues, Romeu Côrtes

doi:10.1590/s0004-282x2007000400002

Cited by 16 publications

(14 citation statements)

References 19 publications

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“…Many authors have investigated the NAWM of patients with MS using DTI. These studies have shown decrease of the FA values in the NAWM, probably secondary to myelin sheath and axonal destruction 4,[9][10][11] . These abnormalities tend to expand the extracellular space, resulting in increased diffusivity and reduced directionality (FA) 3 .…”

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confidence: 89%

Diffusion tensor MR imaging evaluation of the corpus callosum of patients with multiple sclerosis

Rueda

Hygino

Domingues

et al. 2008

Arq. Neuro-Psiquiatr.

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-Objective: To evaluate the fractional anisotropy (FA) values of the normal-appearing white matter of the corpus callosum (CC) in patients with relapsing-remitting multiple sclerosis (MS). Method: Fifty-seven patients with diagnosis of relapsing-remitting MS and 47 age-and gender-matched controls were studied. A conventional MR imaging protocol and a DTI sequence were performed. One neuroradiologist placed the regions of interest (ROIs) in the FA maps in five different portions of the normal-apearing CC (rostrum, genu, anterior and posterior portion of the body and splenium) in all cases. The statistical analysis was performed with the Mann-Whitney U test and p<0.05 was considered statistically significant. Results: The FA values were lower in the MS patients compared with the controls (p<0.05) in the following CC regions: rostrum (0.720 vs 0.819), anterior body (0.698 vs 0.752), posterior body (0.711 vs 0.759) and splenium (0.720 vs 0.880). Conclusion: In this series, there was a robust decrease in the FA in all regions of the normal-appearing CC, being significant in the rostrum, body and splenium. This finding suggests that there is a subtle and diffuse abnormality in the CC, which could be probably related to myelin content loss, axonal damage and gliosis.Key WORDS: multiple sclerosis, diffusion tensor imaging, fractional anisotropy. avaliação do corpo caloso em pacientes com esclerose múltipla através de imagens de rM por tensor de difusãoResumo -Objetivo: Avaliar os valores da anisotropia fracionada (FA) da substância branca aparentemente normal do corpo caloso (CC) em pacientes com esclerose múltipla (eM) remitente recorrente. Método: 57 pacientes com diagnóstico de eM remitente recorrente e 47 controles pareados por sexo e idade foram estudados. O protocolo convencional de RM e imagens de tensor de difusão foram adquiridas. Um neurorradiologista posicionou as regiões de interesse nos mapas de FA em seis porções do CC aparentemente normal (rostro, joelho, anterior e posterior porções do corpo e esplênio) em todos os casos. A análise estatística foi realizada com o teste Mann-Whitney U e p<0,05 foi considerado estatisticamente significativo. Resultados: Os valores de FA foram menores nos pacientes com eM comparados com os controles (p<0,05) nas seguintes porções do CC: rostro (0,720 vs 0,819), corpo anterior (0,698 vs 0,752), corpo posterior (0,711 vs 0,759) e esplênio (0,720 vs 0,880). Conclusão: Na presente série houve redução robusta na FA em todas as regiões aparentemente normais do CC, sendo significativa no rostro, corpo e esplênio. este achado sugere que há alteração difusa no corpo caloso de pacientes com eM, provavelmente relacionada a perda da mielina, lesão axonal e gliose.PAlAvRAS-ChAve: esclerose múltipla, imagens de tensor de difusão, anisotropia fracionada.

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confidence: 89%

Diffusion tensor MR imaging evaluation of the corpus callosum of patients with multiple sclerosis

Rueda

Hygino

Domingues

et al. 2008

Arq. Neuro-Psiquiatr.

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“…Diffusion tensor imaging is particularly sensitive to microstructural changes in white matter, including axonal and myelin damage (162). SLE patients with normal findings on conventional MRI exhibit decreased fractional anisotropy (a measure of the portion of the diffusion tensor that is due to anisotropy) in the frontal white matter and the corpus callosum (17); patients with amyotrophic lateral sclerosis (163), primary lateral sclerosis (164), or MS (165–168) have also been studied with this technique. Magnetization transfer imaging quantifies the exchange of magnetization between macromolecule‐bound proteins in myelin and water protons in biologic tissue (169).…”

Section: Cognitive Assessment Of Patients With Slementioning

confidence: 99%

Mammary serine protease inhibitor inhibits epithelial growth factor‐induced epithelial‐mesenchymal transition of esophageal carcinoma cells

Zhang¹,

Zhou²,

Lei³

et al. 2008

Cancer

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BACKGROUND:By using proteomic technology, the authors previously observed the substantial down‐regulation of mammary serine protease inhibitor (maspin) in esophageal squamous cell carcinoma and metastases. In the current study, they examined the effects of maspin re‐expression in a maspin‐null esophageal cancer cell line EC109 and also investigated the underlying mechanism.METHODS:A cell line with stable maspin expression was established. An epithelial growth factor (EGF)‐induced epithelial‐mesenchymal transition (EMT) model was used to mimic some aspects of the metastatic process in vitro. The effects of maspin reintroduction on EGF‐induced EMT and cell growth characteristics were evaluated. Comparative proteomic analysis of transfected cells versus parental cells was then performed to explore the potential mechanism.RESULTS:The introduction of maspin into EC109 cells was able to inhibit EGF‐induced EMT and altered cell growth characteristics, including the serum dependence, proliferative response to EGF stimulation, and colony formation ability in soft agar, indicating a conversion from a malignant phenotype to a benign phenotype. Proteomic analysis revealed a significant down‐regulation of a group of glycolytic enzymes in maspin‐transfected cells. In addition, maspin‐transfected cells expressed much lower levels of hypoxia‐inducible factor 1α than parental cells or empty vector transfected cells.CONCLUSIONS:Maspin exhibited a metastasis‐suppressive effect, which may be a consequence of the reversal of the malignant phenotype of EC109 cells. The switch of cellular metabolic phenotype to low glycolysis by the gain of maspin function may play a key role in the process. This finding provides additional evidence of the tumor metastasis‐suppressive activity of maspin and may indicate a new direction for future studies of the mechanism of maspin. Cancer 2009. © 2008 American Cancer Society.

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“…Previous studies with DTI have demonstrated reduced fractional anisotropy in the demyelinating plaques as well as in the normal appearing white matter in the brain of MS patients, despite any abnormality in the conventional MR imaging sequences, including T2-weighted image and FlAIR [6][7][8] .…”

Section: Discussionmentioning

confidence: 89%

“…Advanced MR imaging techniques, such as proton spectroscopy and diffusion tensor imaging, have also been studied with some success when aiming to demonstrate a correlation between the imaging findings and the clinical status 20 . Also, the DTI have demonstrated fractional anisotropy abnormalities in the brain white matter 7,8 and in the spinal cord 11,12 , even in regions with normal signal on T2-weighted images, defining the well-known terms called "normal appearing white matter" and "normal appearing spinal cord".…”

Section: Discussionmentioning

confidence: 99%

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Diffusion tensor imaging of the cervical spinal cord of patients with relapsing-remising multiple sclerosis: a study of 41 cases

Cruz

Domingues

Gasparetto

2009

Arq. Neuro-Psiquiatr.

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-Objective: To evaluate the fractional anisotropy (FA) values of the multiple sclerosis (MS) plaques and normal-appearing cervical spinal cord (NASC) by diffusion tensor MRI imaging (DTI). Method: Forty-one patients with relapsing-remising MS and 37 controls were evaluated. All MRI exams were performed using a conventional protocol, as well as diffusion tensor MR imaging. Regions of interest were placed within the spinal cord lesions and in the normal appearing spinal cord adjacent to the plaque. Results: The FA values were statistically reduced in the plaques compared to the surrounding NASC and to equivalent location in controls. A reduction in FA values was also observed in the spinal cord of MS patients without visible lesions on T2WI. Conclusion: We observed reduced fractional anisotropy in the demyelinating plaques and in the NASC of MS patients, corroborating the hypothesis that the histological extension of the MS lesions is more severe than the abnormalities seen in the conventional MRI sequences.Key WoRDS: multiple sclerosis, magnetic resonance imaging, diffusion tensor imaging. imagens por tensor de difusão da medula cervical de pacientes com esclerose múltipla remitenterecorrente: estudo de 41 casos resumo -Objetivo: Avaliar os valores da anisotropia fracionada (FA) em pacientes com esclerose múltipla (eM) nas placas e na medula espinhal aparentemente normal (MeAN). Método: Quarenta e um pacientes com eM remitente-recorrente e 37 controles foram examinados. Todos os exames foram realizados com protocolo convencional, assim como imagens por tensor de difusão. Regiões de interesse foram definidas nas placas da medula espinhal e na MeAN ao redor das placas. Resultados: os valores de FA estavam significativamente reduzidos nas placas, comparados à MeAN ao redor e às regiões equivalentes dos controles. Redução dos valores de FA também foi demonstrada na medula espinhal de pacientes com eM sem lesões visíveis nas imagens de RM pesadas em T2. Conclusão: observamos redução dos valores de anisotropia fracionada nas placas de desmielinização e na MeAN, corroborando a hipótese de que a extensão histológica das lesões na eM é maior que as alterações de sinal vistas nas seqüências convencionais de ressonância magnética.PAlAvRAS-ChAve: esclerose múltipla, ressonância magnética, imagens por tensor de difusão.

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Evaluation of white matter in patients with multiple sclerosis through diffusion tensor magnetic resonance imaging

Cited by 16 publications

References 19 publications

Diffusion tensor MR imaging evaluation of the corpus callosum of patients with multiple sclerosis

Diffusion tensor MR imaging evaluation of the corpus callosum of patients with multiple sclerosis

Mammary serine protease inhibitor inhibits epithelial growth factor‐induced epithelial‐mesenchymal transition of esophageal carcinoma cells

Diffusion tensor imaging of the cervical spinal cord of patients with relapsing-remising multiple sclerosis: a study of 41 cases

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