Inibição da formação de abscesso abdominal em rato: mortalidade por sepse

Rodrigues, Fernando Henrique Oliveira Carmo; Carneiro, Bruno Gustavo Muzzi Carvalho e; Rocha, Renata Figueiredo; Petroianu, Andy

doi:10.1590/s0004-28032005000100011

Cited by 4 publications

(2 citation statements)

References 20 publications

(29 reference statements)

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“…The experimental model is one of the main means to study sepsis of abdominal origin. The study of the sepsis in experimental models can be driven with administration lipopolissacárides (LPS) intravascular, bacterial peritonitis induced by introduction of feces or bacteria in the peritonial cavity, opening of an intestinal segment or cecal ligation and puncture 26,27 . The CLP model was adopted in this work by presenting some advantages, as it is easy reproducible, simple, it is not necessary the standardization of an inocule.…”

Section: Discussionmentioning

confidence: 99%

Effects of simvastatin in abdominal sepsis in rats<A NAME="volta1"></A>

et al. 2006

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Purpose: Statins are widely recognized as hypolipemic drugs, but some studies have observed anti-inflammatory and immunomodulatory effects, known as pleiotropic. The aims of this work was to study possible anti-inflammatory effects of simvastatin in abdominal sepsis. Serum pro-inflammatory cytokines and leukocytes count were determined in an experimental model of abdominal sepsis, using cecal ligation and puncture (CLP) in rats. Methods: Twenty eigth Wistar rats weighing 285±12g were randomly divided in: CLP/Sinvastatin rats (n=7), treated with 10 mg/Kg of oral simvastatin 18 and 2 hs berofe CLP; CLP/Saline group rats (n=7), treated with oral saline; group Sham/Simvastatin (n=7), treated with simvastatin, and group Sham/Saline (n=7), treated with saline. Serum TNF-α, IL-1β and IL-6 by ELISA and total leukocytes, neutrophils, lymphocytes, and eosinophils were determined 24 hs after CLP. ANOVA and Tukey test were used considering significant p<0.05. Results: It was demonstrated that serum TNF-α, IL-1β and IL-6 were respectively 364,8±42pg/mL; 46,3±18pg/mL and 28,4±13pg/mL in CLP/Sinvastatin rats, significantly lower (p<0.05) than in group CLP/Saline (778,5±86pg/ml; 176,9±46pg/ ml; 133,6±21 pg/ml, respectively). The same results were observed in total leukocytes and neutrophils counts. Conclusion: These results clearly demonstrate that simvastatin is an effective agent that reduces cytokines levels and leukocyte count in sepsis, independently of its well-known lipid-lowering effects. Thus, HMG-CoA reductase inhibitors like simvastatin have important anti-inflammatory effects in abdominal sepsis in rats. Key words: Statin. Inflammation. Abdominal sepsis. Wistar rat. Cytokine. Leukocyte. RESUMO Objetivo:As estatinas são agentes reconhecidamente hipolipemiantes. Vários estudos têm revelado que eles têm ações pleiotrópicas, como antiinflamatória e imunomoduladora. Tentando-se entender o papel antiinflamatório da sinvastatina na sepse, foram analisados os níveis de citocinas pró-inflamatórias e contagem de leucócitos em modelo de sepse abdominal por ligadura e punção do ceco (LPC) em ratos. Métodos: Foram utilizados 28 ratos Wistar pesando 285±12g, assim divididos: grupo sepse (n=14), submetidos a LPC e grupo sham (n=14), submetidos a laparotomia e manipulação suave do ceco. No grupo LPC/sinvastatina (n=7) os ratos receberam 10mg/kg de sinvastatina via oral 18 e 2 horas antes da LPC e no grupo LPC/salina (n=7) os ratos receberam injeção oral de solução salina 0,9 %. Os animais dos grupos sham/sinvastatina (n=7) e sham/salina (n=7) receberam o mesmo tratamento. Dosagem de TNF-α, IL-1β e IL-6 por ELISA e contagem de leucócitos totais, neutrófilos, linfócitos e eosinófilos foram realizadas em todos os animais. Análise estatística foi feita pelo ANOVA e teste de Tukey, com significância p<0,05. Resultados: Ficou demonstrado que as dosagens de TNF-α, IL-1β e IL-6 atingiram valores de 364,8±42pg/ml; 46,3±18pg/ml e 28,4±13pg/ml no grupo submetido à sepse e tratados com sinvastatina, significantemente mais baixos do que...

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Section: Discussionmentioning

confidence: 99%

Effects of simvastatin in abdominal sepsis in rats<A NAME="volta1"></A>

et al. 2006

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“…In our study, a model of subcutaneous abscess was chosen because of its circumscribed nature and easy delineation on PET images, and because of its high accessibility for ex‐vivo analysis. This model is very simply obtained while injecting caecal contents subcutaneously in the flank from rodents . Two to 10 days later, it may be easily recognized as a tight nodule with signs of intense local inflammatory response and with neutrophil recruitment, interstitial edema, and disruption of epithelial integrity.…”

Section: Resultsmentioning

confidence: 99%

Development of 6‐[¹⁸F]fluoro‐carbohydrate‐based prosthetic groups and their conjugation to peptides via click chemistry

Collet

Maskali

Clément

et al. 2015

Labelled Comp Radiopharmac

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This work describes the development of new 6-[(18) F]fluoro-carbohydrate-based prosthetic groups equipped with an azido arm that are able to participate in copper(I)-catalyzed cycloadditions for (18) F labeling of biomolecules under mild conditions. The radiolabeling in high radiochemical yields (up to 68 ± 6%) of these different prosthetic groups is presented. The flexibility of the azido arm introduced on the carbohydrate moieties allows efficient click reactions with different alkyne functionalized peptides such as gluthation or Arg-Gly-Asp derivatives in order to prepare glycopeptides. The radiosyntheses of (18) F-labeled glycopeptides proceed in high radiochemical yields (up to 76%) in an automated process with excellent radiochemical purity. The addition of a sugar moiety on peptides should enhance the bioavailability, pharmacokinetic, and in vivo clearance properties of these glycopeptides, compared with the unlabeled native peptide, and these properties are highly favorable for positron emission tomography imaging. A high uptake of (18) F-β-gluco-c(RGDfC) is shown by positron emission tomography imaging in a subcutaneous abscess model in the rat, revealing the potential of this tracer to monitor integrin expression as a part of inflammation and/or angiogenesis processes.

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Effects of Topical Treatment With Euphorbia Tirucalli Latex on the Survival and Intestinal Adhesions in Rats With Experimental Peritonitis

Araújo

Mrué

Neves

et al. 2015

ABCD, arq. bras. cir. dig.

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Background: The use of plants of the family Euphorbiaceae, particularly Euphorbia tirucalli (avelós) has been popularly widespread for treating a variety of diseases of infectious, tumoral, and inflammatory. Aim: To demonstrated antimicrobial and immunomodulatory effects of these extracts, evaluating the effect of a topical treatment with an aqueous solution of avelós latex on the survival and on intestinal adhesions in rats with experimental peritonitis. Methods: Peritonitis was induced in 24 Wistar rats, that were randomized into four groups of six as follows: (1) Control group (n=6), no treatment; (2) Antibiotic group (n=6), treatment with a single intramuscular dose of antibiotic Unasyn; (3) Saline group (n=6), the abdominal cavity was washed with 0.9% saline; and (4) E.tirucalli group (n=6), the abdominal cavity was washed with E. tirucalli at a concentration of 12 mg/ml. The animals that died were necropsied, and the time of death was recorded. The survivors were killed on postoperative day 11, and necropsy was subsequently performed for evaluation of the intestinal adhesions. Results: Significant differences were observed in the control and antibiotic groups (p<0.01) with respect to the survival hours when compared with the saline and E. tirucalli groups. There was no significant difference (p>0.05) in the survival of animals in the saline andE. tirucalli groups; however, one animal died in the saline group. Necropsy of the animals in the saline and E. tirucalligroups showed strong adhesions resistant to manipulation, between the intestinal loops and abdominal wall. The remaining groups did not show any adhesions. Conclusions: Topical treatment with E. tirucalli latex stimulated an increased formation of intestinal adhesions and prevented the death of all animals with peritonitis.

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Inibição da formação de abscesso abdominal em rato: mortalidade por sepse

Cited by 4 publications

References 20 publications

Effects of simvastatin in abdominal sepsis in rats<A NAME="volta1"></A>

Effects of simvastatin in abdominal sepsis in rats<A NAME="volta1"></A>

Development of 6‐[¹⁸F]fluoro‐carbohydrate‐based prosthetic groups and their conjugation to peptides via click chemistry

Effects of Topical Treatment With Euphorbia Tirucalli Latex on the Survival and Intestinal Adhesions in Rats With Experimental Peritonitis

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Inibição da formação de abscesso abdominal em rato: mortalidade por sepse

Cited by 4 publications

References 20 publications

Effects of simvastatin in abdominal sepsis in rats<A NAME="volta1"></A>

Effects of simvastatin in abdominal sepsis in rats<A NAME="volta1"></A>

Development of 6‐[18F]fluoro‐carbohydrate‐based prosthetic groups and their conjugation to peptides via click chemistry

Effects of Topical Treatment With Euphorbia Tirucalli Latex on the Survival and Intestinal Adhesions in Rats With Experimental Peritonitis

Contact Info

Product

Resources

About

Development of 6‐[¹⁸F]fluoro‐carbohydrate‐based prosthetic groups and their conjugation to peptides via click chemistry