Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia

Forones, Nora Manoukian; Carvalho, Ana Paula Souza; Giannotti-Filho, Oswaldo; Lourenço, Laércio Gomes; Oshima, Celina Tizuko Fujiyama

doi:10.1590/s0004-28032005000100008

Cited by 29 publications

(21 citation statements)

References 17 publications

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“…According to our fi ndings, increased proliferative activity in gastric cancer is associated with increased level of apoptosis. These fi ndings are agreeing with the literature data [8,9].…”

Section: Resultssupporting

confidence: 94%

“…According to N. M. Fonores et al [8] the indices of cell proliferation and apoptosis progressively increase with increasing the depth of the intestinal-type gastric adenocarcinoma invasion. L. Xiao et al [9] indicated that the intestinal-type gastric adenocarcinoma is characterized by the higher expression level of caspase 3 and p53 (in comparison with the diffuse-type gastric adenocarcinoma), but the correlation between the expression levels of Ki-67, p53, caspase 3 and the depth of the tumor invasion was not identifi ed.…”

Section: материалы и методыmentioning

confidence: 99%

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Comparative immunohistochemical characteristic of expression levels of Ki-67, p53, caspase 3 in non-invasive and invasive intestinal-type gastric adenocarcinoma

Tumanskiy

Khrystenko

2016

Patologìâ

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Aim.Comparison analysis of expression levels of p53, and caspase 3 was performed between the tumor cells and the stromal cells of non-invasive intestinal-type gastric adenocarcinoma on the one hand and the tumor cells and the stromal cells of invasive intestinal-type gastric adenocarcinoma on the other hand.Materials and methods. For this purpose, pathohistological and immunohistochemical studies were used. The surgical material of 10 patients with non-invasive (pTis, pT1) intestinal-type gastric adenocarcinoma and 10 patients with invasive (pT2, pT3, pT4) intestinaltype gastric adenocarcinoma were analyzed.Results. It was established that intestinal-type gastric adenocarcinoma is characterized by the medium Ki-67 labeling index, the low expression level of p53, the low expression level of caspase 3 in the tumor cells, and also the low Ki-67 labeling index and the extremely low expression level of caspase 3 in the stromal cells. The tumor cells of invasive intestinal-type gastric adenocarcinoma are characterized by the 2 times higher expression level of p53, than the tumor cells of non-invasive intestinal-type gastric adenocarcinoma. Moreover, direct correlation between the medium Ki-67 labeling index of the tumor cells and the low Ki-67 labeling index of the stromal cells, and also direct correlation between the medium Ki-67 labeling index in the tumor cells of intestinal-type gastric adenocarcinoma and the low expression level of p53 in these cells were identifi ed.Conclusions. These data demonstrate a signifi cantly more activity of proliferation and apoptosis in the tumor cells of intestinal-type gastric adenocarcinoma, in comparison with the stromal cells of this tumor. Invasive intestinal-type gastric adenocarcinoma differs by the 2 times higher expression level of p53, in comparison with the same index for non-invasive carcinoma. Furthermore, a tight association between proliferative processes in the tumor cells and in the stromal cells of intestinal-type gastric adenocarcinoma, and also an association between proliferative processes and accumulation of mutant protein p53 in the tumor cells of intestinal-type gastric adenocarcinoma were identifi ed.Порівняльна імуногістохімічна характеристика експресії Ki-67, p53, каспази-3 в неінвазивній та інвазивній аденокарциномі шлунка кишкового типу В. О. Туманський, Т. О. Христенко Мета роботи -порівняти рівні експресії маркерів Ki-67, p53, каспази-3 в пухлинних клітинах і клітинах строми неінвазив-ної та інвазивної аденокарциноми шлунка кишкового типу, а також дослідити наявність кореляційних зв'язків між рівнями експресії відзначених маркерів.Матеріали та методи. Здійснили патогістологічне та імуногістохімічне дослідження операційного матеріалу 10 хворих на неінвазивну (pTis, pT1) аденокарциному шлунка кишкового типу, а також 10 хворих на інвазивну (pT2, pT3, pT4) аденокарци-ному шлунка кишкового типу.Результати. Встановлено, що аденокарцинома шлунка кишкового типу характеризується середнім індексом проліферації, низьким рівнем експресії р53, низьким рівнем експ...

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Section: Resultssupporting

confidence: 94%

Section: материалы и методыmentioning

confidence: 99%

Comparative immunohistochemical characteristic of expression levels of Ki-67, p53, caspase 3 in non-invasive and invasive intestinal-type gastric adenocarcinoma

Tumanskiy

Khrystenko

2016

Patologìâ

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“…This alteration is involved in the carcinogenesis of gastric cancer, although this is not an independent factor (44) . Some authors suggest its relation to late events of gastric cancer (8,15) . Conversely, Ki-67 is a marker of cell proliferation and related to the prognosis of gastric cancer (70) .…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Mucin Phenotype Can Predict Gastric Cancer Recurrence After Endoscopic Mucosal Resection

Hondo

Kishi

Safatle‐Ribeiro

et al. 2017

Arq. Gastroenterol.

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-Background -Endoscopic mucosal resection is still considered an accepted treatment for early gastric cancer for selected cases. Histopathologic criteria for curative endoscopic resection are intramucosal well-differentiated adenocarcinoma, lateral and deep margins free of tumor, no histological ulceration, and no venous or lymphatic embolism. A 5% local recurrence rate has been described even when all the above-mentioned criteria are met. On the other hand, antigen expression by tumoral cells has been related to the biological behavior of several tumors. Objective -To evaluate whether early gastric cancer mucin immunoexpression, p53 and Ki-67, can predict recurrence after endoscopic mucosal resection, even when standard histopathologic criteria for curative measures have been attempted. Methods -Twenty-two patients with early gastric cancer were considered to have been completely resected by endoscopic mucosal resection. Local recurrence occurred in 5/22 (22.7%). Immunohistochemical study was possible in 18 (81.8%) resected specimens. Patients were divided in two groups: those with and those without local recurrence. They were compared across demographic, endoscopic, histologic data, and immunohistochemical factors for MUC2, MUC5a, CD10, p53, and Ki-67. Results -Mucin immunoexpression allowed a reclassification of gastric adenocarcinoma in intestinal (10), gastric (2), mixed (4), and null phenotypes (2). Mixed phenotype (positive for both MUC2 and MUC5a) was found in 80% of cases in the local recurrence group, while the intestinal type (positive MUC2 and negative MUC5a) was found in 76.9% of cases without local recurrence (P=0.004). Other observed features did not correlate with neoplastic recurrence. Conclusion -The mixed phenotype of early gastric adenocarcinoma is associated with a higher probability of local recurrence after endoscopic mucosal resection.HEADINGS -Stomach neoplasms. Endoscopic mucosal resection. Gastric mucins.

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“…Gastric cancer is not only a disease with abnormal cell proliferation and differentiation, but also a disease with abnormal apoptosis [16,17] . Increased apoptosis in human gastric cancer cells could be observed after treatment with 5-fluorouracil, cisplatin, etc.…”

Section: Figure 1 Propidium Iodide (Pi) Staining For Flow Cytometric mentioning

confidence: 99%

Effect of 2-(8-hydroxy-6-methoxy-1-oxo-1H-2-benzopyran-3-yl) propionic acid in combination with carboplatin on gastric carcinoma growthin vivo

Chen

Zhu²,

Hong³

et al. 2007

WJG

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Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia

Abstract: The positivity of bcl-2 was higher in metaplasia and probably is involved in the progression of carcinogenesis. p53 was negative in metaplasia and positive in more than half of the gastric cancer, mostly in stage IV, suggesting a late event in gastric cancer.

Cited by 29 publications

References 17 publications

Comparative immunohistochemical characteristic of expression levels of Ki-67, p53, caspase 3 in non-invasive and invasive intestinal-type gastric adenocarcinoma

Comparative immunohistochemical characteristic of expression levels of Ki-67, p53, caspase 3 in non-invasive and invasive intestinal-type gastric adenocarcinoma

Characterization of the Mucin Phenotype Can Predict Gastric Cancer Recurrence After Endoscopic Mucosal Resection

Effect of 2-(8-hydroxy-6-methoxy-1-oxo-1H-2-benzopyran-3-yl) propionic acid in combination with carboplatin on gastric carcinoma growthin vivo

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