Participação da apoptose na rejeição aguda do transplante intestinal em ratos

Abstract

Isogenic rats Brown-Norway (BN) and Lewis (LEW) were submitted to intestinal heterotopic allotransplantation and divided in two groups: LEW donor to LEW recipient isograft group C and BN donor to LEW recipient allograft group (Tx). According to the day of sacrifice, Tx group were subdivided in three subgroups with eight animals each as follow: Tx3-- sacrificed at third postoperative day (POD), Tx5 -- sacrificed at fifth POD and Tx7 -- sacrificed at seventh POD. Eight animals from control group were subdivided in three moments according to the time of biopsy from the graft as follow: C3 -- biopsy at third POD; C5 -- biopsy at fifth POD and C7 -- biopsy at seventh POD. All animals from control group were sacrificed at seventh POD. Rejection parameters were compared between the control groups (C3 vs C5, C3 vs C7 and C5 vs C7, and allograft group (Tx3 vs Tx5, Tx3 vs Tx7 and Tx5 vs Tx7). The same parameters were analyzed between the control group and allograft groups ( C3 vs Tx3, C5 vs Tx5 and C7 vs Tx7). In C group no statistical significant difference regarding the expression of the apoptotic cells were detected, while in Tx group, the presence of apoptotic cells were remarkable since the third postoperative day.

Transplante experimental, subcutâneo e intraperitoneal, de ovário em suínos: estudo histomorfométrico e imunoistoquímico

“…Na necrose, há alterações precoces da membrana plasmática em resposta rápida a agressões do meio, com aumento da permeabilidade da camada de fosfolípides e ruptura física da membrana plasmática (20) . Em contraste, a apoptose ou morte celular programada caracteriza-se pela condensação nuclear e citoplasmática, modificações no citoesqueleto com formação de bolhas e de corpos apoptóticos envoltos por membrana plasmática, sem desintegração de organelas e sem infiltrado leucocitário (20,21) . O uso de suíno em pesquisas científicas é prática antiga; na literatura, já estão estabelecidas as semelhanças entre anatomia, fisiologia e fisiopatologia do suíno e do homem (22) .…”